Metabolic profiling of probiotic strain Lactobacillus delbrueckii subsp. bulgaricus L14 cultivated in presence of prebiotic oligosaccharides and polysaccharides in simulating in vitro gastrointestinal tract system
AbstractThis study examined the effect of lactulose, galactooligosaccharide, fructooligosaccharide, inulin, and β-glucan on the probiotic strain Lactobacillus delbrueckii subsp. bulgaricus L14, cultivated in an in vitro gastrointestinal system model. We analyzed the degree of hydrolysis of the studi...
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| Format: | Article |
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Taylor & Francis Group
2023-12-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2023.2178825 |
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| author | Ivica Dimov Daniela Mollova Tonka Vasileva Veselin Bivolarski Mariana Nikolova Anelia Bivolarska Ilia Iliev |
| author_facet | Ivica Dimov Daniela Mollova Tonka Vasileva Veselin Bivolarski Mariana Nikolova Anelia Bivolarska Ilia Iliev |
| author_sort | Ivica Dimov |
| collection | DOAJ |
| description | AbstractThis study examined the effect of lactulose, galactooligosaccharide, fructooligosaccharide, inulin, and β-glucan on the probiotic strain Lactobacillus delbrueckii subsp. bulgaricus L14, cultivated in an in vitro gastrointestinal system model. We analyzed the degree of hydrolysis of the studied prebiotic oligosaccharides in condition of simulated gastric fluid. The results showed that lactulose had the highest resistance, galactooligosaccharide underwent hydrolysis, and fructooligosaccharide was the most sensitive. Among the polysaccharides, fructose was released from inulin and glucose from β-glucan. Short-chain oligosaccharides and metabolites derived from studied prebiotic oligosaccharides and polysaccharides, supported the growth of probiotic strain L14, which showed the highest growth with fructooligosaccharides and β-glucan as carbohydrate sources. The profile of the activated enzymes secreted by the probiotic strain L14, indicated their inducible character. Beta-galactosidase was activated in the presence of lactulose and GalOS, inulinase was activated in the presence of inulin and fructooligosaccharides, and β-glucosidase was activated in the presence of β-glucan fragments. Analysis of the produced organic and short-chain fatty acids showed that the typical representative of the homofermentative lactobacilli Lb. delbrueckii subsp. bulgaricus L14 changes its metabolism from a homofermentative to a heterofermentative type, best expressed in the presence of lactulose, galactooligosaccharide, and β-glucan. |
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| id | doaj.art-1f8a4b7d77b142e5a99925793080ceb2 |
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| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-03-08T17:18:33Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-1f8a4b7d77b142e5a99925793080ceb22024-01-03T09:44:11ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302023-12-0137126027210.1080/13102818.2023.2178825Metabolic profiling of probiotic strain Lactobacillus delbrueckii subsp. bulgaricus L14 cultivated in presence of prebiotic oligosaccharides and polysaccharides in simulating in vitro gastrointestinal tract systemIvica Dimov0Daniela Mollova1Tonka Vasileva2Veselin Bivolarski3Mariana Nikolova4Anelia Bivolarska5Ilia Iliev6Department of Medical Biochemistry, Faculty of Pharmacy, Medical University of Plovdiv, Plovdiv, BulgariaDepartment of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University “Paisii Hilendarski”, Plovdiv, BulgariaDepartment of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University “Paisii Hilendarski”, Plovdiv, BulgariaDepartment of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University “Paisii Hilendarski”, Plovdiv, BulgariaDepartment of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University “Paisii Hilendarski”, Plovdiv, BulgariaDepartment of Medical Biochemistry, Faculty of Pharmacy, Medical University of Plovdiv, Plovdiv, BulgariaDepartment of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University “Paisii Hilendarski”, Plovdiv, BulgariaAbstractThis study examined the effect of lactulose, galactooligosaccharide, fructooligosaccharide, inulin, and β-glucan on the probiotic strain Lactobacillus delbrueckii subsp. bulgaricus L14, cultivated in an in vitro gastrointestinal system model. We analyzed the degree of hydrolysis of the studied prebiotic oligosaccharides in condition of simulated gastric fluid. The results showed that lactulose had the highest resistance, galactooligosaccharide underwent hydrolysis, and fructooligosaccharide was the most sensitive. Among the polysaccharides, fructose was released from inulin and glucose from β-glucan. Short-chain oligosaccharides and metabolites derived from studied prebiotic oligosaccharides and polysaccharides, supported the growth of probiotic strain L14, which showed the highest growth with fructooligosaccharides and β-glucan as carbohydrate sources. The profile of the activated enzymes secreted by the probiotic strain L14, indicated their inducible character. Beta-galactosidase was activated in the presence of lactulose and GalOS, inulinase was activated in the presence of inulin and fructooligosaccharides, and β-glucosidase was activated in the presence of β-glucan fragments. Analysis of the produced organic and short-chain fatty acids showed that the typical representative of the homofermentative lactobacilli Lb. delbrueckii subsp. bulgaricus L14 changes its metabolism from a homofermentative to a heterofermentative type, best expressed in the presence of lactulose, galactooligosaccharide, and β-glucan.https://www.tandfonline.com/doi/10.1080/13102818.2023.2178825Lactobacillus bulgaricusprobioticsprebioticsβ-glucanβ-galactosidaseinulinase |
| spellingShingle | Ivica Dimov Daniela Mollova Tonka Vasileva Veselin Bivolarski Mariana Nikolova Anelia Bivolarska Ilia Iliev Metabolic profiling of probiotic strain Lactobacillus delbrueckii subsp. bulgaricus L14 cultivated in presence of prebiotic oligosaccharides and polysaccharides in simulating in vitro gastrointestinal tract system Biotechnology & Biotechnological Equipment Lactobacillus bulgaricus probiotics prebiotics β-glucan β-galactosidase inulinase |
| title | Metabolic profiling of probiotic strain Lactobacillus delbrueckii subsp. bulgaricus L14 cultivated in presence of prebiotic oligosaccharides and polysaccharides in simulating in vitro gastrointestinal tract system |
| title_full | Metabolic profiling of probiotic strain Lactobacillus delbrueckii subsp. bulgaricus L14 cultivated in presence of prebiotic oligosaccharides and polysaccharides in simulating in vitro gastrointestinal tract system |
| title_fullStr | Metabolic profiling of probiotic strain Lactobacillus delbrueckii subsp. bulgaricus L14 cultivated in presence of prebiotic oligosaccharides and polysaccharides in simulating in vitro gastrointestinal tract system |
| title_full_unstemmed | Metabolic profiling of probiotic strain Lactobacillus delbrueckii subsp. bulgaricus L14 cultivated in presence of prebiotic oligosaccharides and polysaccharides in simulating in vitro gastrointestinal tract system |
| title_short | Metabolic profiling of probiotic strain Lactobacillus delbrueckii subsp. bulgaricus L14 cultivated in presence of prebiotic oligosaccharides and polysaccharides in simulating in vitro gastrointestinal tract system |
| title_sort | metabolic profiling of probiotic strain lactobacillus delbrueckii subsp bulgaricus l14 cultivated in presence of prebiotic oligosaccharides and polysaccharides in simulating in vitro gastrointestinal tract system |
| topic | Lactobacillus bulgaricus probiotics prebiotics β-glucan β-galactosidase inulinase |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2023.2178825 |
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