A comparative karyological study of Helianthemum (Cistaceae): karyotype size, karyotype symmetry and evolution of chromosome number
In this study we assessed karyotype size and symmetry for a comprehensive taxonomic and geographic representation of Helianthemum and reconstructed chromosome number evolution in the genus. Using root tips, we photographed mitotic metaphase spreads to obtain chromosome number, total haploid (monoplo...
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| Format: | Article |
| Language: | English |
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Consejo Superior de Investigaciones Científicas
2023-06-01
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| Series: | Anales del Jardín Botánico de Madrid |
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| Online Access: | https://rjb.revistas.csic.es/index.php/rjb/article/view/576 |
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| author | Sara Martín Hernanz Rafael González Albaladejo Encarnación Rubio Pérez Polina Volkova Mohamed Djamel Miara Deniz Ulukuș Mehmet Sezgin Abelardo Aparicio Martínez |
| author_facet | Sara Martín Hernanz Rafael González Albaladejo Encarnación Rubio Pérez Polina Volkova Mohamed Djamel Miara Deniz Ulukuș Mehmet Sezgin Abelardo Aparicio Martínez |
| author_sort | Sara Martín Hernanz |
| collection | DOAJ |
| description | In this study we assessed karyotype size and symmetry for a comprehensive taxonomic and geographic representation of Helianthemum and reconstructed chromosome number evolution in the genus. Using root tips, we photographed mitotic metaphase spreads to obtain chromosome number, total haploid (monoploid) length of the chromosome set (THL), karyotype formula, Stebbins’ classification of karyotype asymmetry, interchromosomal coefficient of variation of chromosome length (CVCL) and intrachromosomal mean centromeric asymmetry (MCA) using MATO (Measurement and Analysis Tools). We found that shifts in chromosome number are not a major driver in the evolution of Helianthemum, whose chromosome number evolved at a constant rate of single chromosome gain or loss. Karyotype asymmetry is very low and little variable in all taxonomic categories studied, with a predominance of metacentric and submetacentric small to medium-sized chromosomes about 3 μm at the genus level. However, total karyotype length varies from 16.91 μm to 47.84 μm at the species level, with a cytogenetic signature that is not conserved within subgenera and most sections. Overall, H. subg. Plectolobum shows both the longest and the most symmetrical karyotypes. We hypothesize that the variation in karyotype size in Helianthemum is likely a consequence of chromosome rearrangements that have occurred under selective pressures.
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| first_indexed | 2024-03-12T14:25:01Z |
| format | Article |
| id | doaj.art-1f8c740868074052bc490982b1189611 |
| institution | Directory Open Access Journal |
| issn | 0211-1322 1988-3196 |
| language | English |
| last_indexed | 2024-03-12T14:25:01Z |
| publishDate | 2023-06-01 |
| publisher | Consejo Superior de Investigaciones Científicas |
| record_format | Article |
| series | Anales del Jardín Botánico de Madrid |
| spelling | doaj.art-1f8c740868074052bc490982b11896112023-08-18T10:01:35ZengConsejo Superior de Investigaciones CientíficasAnales del Jardín Botánico de Madrid0211-13221988-31962023-06-0180110.3989/ajbm.576A comparative karyological study of Helianthemum (Cistaceae): karyotype size, karyotype symmetry and evolution of chromosome numberSara Martín Hernanz0Rafael González Albaladejo1Encarnación Rubio Pérez2Polina Volkova3Mohamed Djamel Miara4Deniz Ulukuș5Mehmet Sezgin6Abelardo Aparicio Martínez7Departamento de Biología Vegetal y Ecología, Universidad de Sevilla - Royal Botanic GardensDepartamento de Biología Vegetal y Ecología, Universidad de SevillaDepartamento de Biología Vegetal y Ecología, Universidad de SevillaPapanin Institute for Biology of Inland Waters, Russian Academy of SciencesFaculty of Nature and Life Sciences, Laboratory of Agro-Biotechnology and Nutrition in Semi-arid Areas, Ibn Khaldoun University of TiaretDepartment of Biotechnology, Faculty of Science, Selçuk UniversityBiology Department, Faculty of Science, Çankırı Karatekin UniversityDepartamento de Biología Vegetal y Ecología, Universidad de SevillaIn this study we assessed karyotype size and symmetry for a comprehensive taxonomic and geographic representation of Helianthemum and reconstructed chromosome number evolution in the genus. Using root tips, we photographed mitotic metaphase spreads to obtain chromosome number, total haploid (monoploid) length of the chromosome set (THL), karyotype formula, Stebbins’ classification of karyotype asymmetry, interchromosomal coefficient of variation of chromosome length (CVCL) and intrachromosomal mean centromeric asymmetry (MCA) using MATO (Measurement and Analysis Tools). We found that shifts in chromosome number are not a major driver in the evolution of Helianthemum, whose chromosome number evolved at a constant rate of single chromosome gain or loss. Karyotype asymmetry is very low and little variable in all taxonomic categories studied, with a predominance of metacentric and submetacentric small to medium-sized chromosomes about 3 μm at the genus level. However, total karyotype length varies from 16.91 μm to 47.84 μm at the species level, with a cytogenetic signature that is not conserved within subgenera and most sections. Overall, H. subg. Plectolobum shows both the longest and the most symmetrical karyotypes. We hypothesize that the variation in karyotype size in Helianthemum is likely a consequence of chromosome rearrangements that have occurred under selective pressures. https://rjb.revistas.csic.es/index.php/rjb/article/view/576ChromEvolcomparative cytogeneticskaryotypeplant cytotaxonomy |
| spellingShingle | Sara Martín Hernanz Rafael González Albaladejo Encarnación Rubio Pérez Polina Volkova Mohamed Djamel Miara Deniz Ulukuș Mehmet Sezgin Abelardo Aparicio Martínez A comparative karyological study of Helianthemum (Cistaceae): karyotype size, karyotype symmetry and evolution of chromosome number Anales del Jardín Botánico de Madrid ChromEvol comparative cytogenetics karyotype plant cytotaxonomy |
| title | A comparative karyological study of Helianthemum (Cistaceae): karyotype size, karyotype symmetry and evolution of chromosome number |
| title_full | A comparative karyological study of Helianthemum (Cistaceae): karyotype size, karyotype symmetry and evolution of chromosome number |
| title_fullStr | A comparative karyological study of Helianthemum (Cistaceae): karyotype size, karyotype symmetry and evolution of chromosome number |
| title_full_unstemmed | A comparative karyological study of Helianthemum (Cistaceae): karyotype size, karyotype symmetry and evolution of chromosome number |
| title_short | A comparative karyological study of Helianthemum (Cistaceae): karyotype size, karyotype symmetry and evolution of chromosome number |
| title_sort | comparative karyological study of helianthemum cistaceae karyotype size karyotype symmetry and evolution of chromosome number |
| topic | ChromEvol comparative cytogenetics karyotype plant cytotaxonomy |
| url | https://rjb.revistas.csic.es/index.php/rjb/article/view/576 |
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