Molecular genetic methods of analysis to identify the species affiliation of the raw material composition in food products
Methods based on the analysis of proteins and DNA molecules are more and more used to assess the composition of food products. Proteins research methods include immunological, electrophoretic and chromatographic ones. The analysis of DNA molecules is most often used to identify the species affiliati...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Russian |
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Murmansk State Technical University
2020-09-01
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| Series: | Vestnik MGTU |
| Subjects: | |
| Online Access: | http://vestnik.mstu.edu.ru/show-eng.shtml?art=2068 |
| _version_ | 1819228580286038016 |
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| author | Yurova E. A. Zhizhin N. A. Filchakova S. A. |
| author_facet | Yurova E. A. Zhizhin N. A. Filchakova S. A. |
| author_sort | Yurova E. A. |
| collection | DOAJ |
| description | Methods based on the analysis of proteins and DNA molecules are more and more used to assess the composition of food products. Proteins research methods include immunological, electrophoretic and chromatographic ones. The analysis of DNA molecules is most often used to identify the species affiliation of food components. This is due to the stability of their structure compared to proteins, as well as their presence in most biological tissues. The results of studies evaluating methodological approaches for the application of the PCR diagnostic method to identify the composition of food products and the possibility of their use for monitoring dairy products have been shown. The objects of research were samples of cow, goat, sheep milk, as well as milk samples of different animal species mixed in various ratios. DNA was extracted from milk samples according to a unified technique for the separation of DNA molecules in milk and dairy products. The work also considers the possibility of using the PCR diagnostic method to identify the raw material origin of the product. To evaluate the measurement methods, artificially created samples of raw milk were used, which were cow, goat and sheep milk, a mix of three types of milk in different ratios. As a result of the research, the main method has been chosen as the real time PCR method, which has reliability, high sensitivity, sufficient rapidity, with the possibility of using it for dairy multicomponent products with a complex structural matrix, as well as products that have undergone deep technological processing. |
| first_indexed | 2024-12-23T10:59:32Z |
| format | Article |
| id | doaj.art-1f8f06114434466595093b47218722a1 |
| institution | Directory Open Access Journal |
| issn | 1560-9278 1997-4736 |
| language | Russian |
| last_indexed | 2024-12-23T10:59:32Z |
| publishDate | 2020-09-01 |
| publisher | Murmansk State Technical University |
| record_format | Article |
| series | Vestnik MGTU |
| spelling | doaj.art-1f8f06114434466595093b47218722a12022-12-21T17:49:41ZrusMurmansk State Technical UniversityVestnik MGTU1560-92781997-47362020-09-0123321422310.21443/1560-9278-2020-23-3-214-223Molecular genetic methods of analysis to identify the species affiliation of the raw material composition in food productsYurova E. A. 0https://orcid.org/0000-0003-3369-5673Zhizhin N. A.1https://orcid.org/0000-0002-0876-8968Filchakova S. A. 2https://orcid.org/0000-0001-6563-9700All-Russian Dairy Research InstituteAll-Russian Dairy Research InstituteAll-Russian Dairy Research InstituteMethods based on the analysis of proteins and DNA molecules are more and more used to assess the composition of food products. Proteins research methods include immunological, electrophoretic and chromatographic ones. The analysis of DNA molecules is most often used to identify the species affiliation of food components. This is due to the stability of their structure compared to proteins, as well as their presence in most biological tissues. The results of studies evaluating methodological approaches for the application of the PCR diagnostic method to identify the composition of food products and the possibility of their use for monitoring dairy products have been shown. The objects of research were samples of cow, goat, sheep milk, as well as milk samples of different animal species mixed in various ratios. DNA was extracted from milk samples according to a unified technique for the separation of DNA molecules in milk and dairy products. The work also considers the possibility of using the PCR diagnostic method to identify the raw material origin of the product. To evaluate the measurement methods, artificially created samples of raw milk were used, which were cow, goat and sheep milk, a mix of three types of milk in different ratios. As a result of the research, the main method has been chosen as the real time PCR method, which has reliability, high sensitivity, sufficient rapidity, with the possibility of using it for dairy multicomponent products with a complex structural matrix, as well as products that have undergone deep technological processing.http://vestnik.mstu.edu.ru/show-eng.shtml?art=2068milkdairy productsmeat productsfish productsmolecular genetic research methodsмолокомолочная продукциямясная продукциярыбная продукциямолекулярно-генетические методы исследования |
| spellingShingle | Yurova E. A. Zhizhin N. A. Filchakova S. A. Molecular genetic methods of analysis to identify the species affiliation of the raw material composition in food products Vestnik MGTU milk dairy products meat products fish products molecular genetic research methods молоко молочная продукция мясная продукция рыбная продукция молекулярно-генетические методы исследования |
| title | Molecular genetic methods of analysis to identify the species affiliation of the raw material composition in food products |
| title_full | Molecular genetic methods of analysis to identify the species affiliation of the raw material composition in food products |
| title_fullStr | Molecular genetic methods of analysis to identify the species affiliation of the raw material composition in food products |
| title_full_unstemmed | Molecular genetic methods of analysis to identify the species affiliation of the raw material composition in food products |
| title_short | Molecular genetic methods of analysis to identify the species affiliation of the raw material composition in food products |
| title_sort | molecular genetic methods of analysis to identify the species affiliation of the raw material composition in food products |
| topic | milk dairy products meat products fish products molecular genetic research methods молоко молочная продукция мясная продукция рыбная продукция молекулярно-генетические методы исследования |
| url | http://vestnik.mstu.edu.ru/show-eng.shtml?art=2068 |
| work_keys_str_mv | AT yurovaea moleculargeneticmethodsofanalysistoidentifythespeciesaffiliationoftherawmaterialcompositioninfoodproducts AT zhizhinna moleculargeneticmethodsofanalysistoidentifythespeciesaffiliationoftherawmaterialcompositioninfoodproducts AT filchakovasa moleculargeneticmethodsofanalysistoidentifythespeciesaffiliationoftherawmaterialcompositioninfoodproducts |