Biological activities of extracts from <it>Chenopodium ambrosioides</it> Lineu and <it>Kielmeyera neglecta</it> Saddi
<p>Abstract</p> <p>Background</p> <p><it>Chenopodium ambrosioides and Kielmeyera neglecta</it> are plants traditionally used in Brazil to treat various infectious diseases. The study of the biological activities of these plants is of great importance for the...
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| Format: | Article |
| Language: | English |
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BMC
2012-07-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Subjects: | |
| Online Access: | http://www.ann-clinmicrob.com/content/11/1/20 |
| _version_ | 1818207505427202048 |
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| author | Sousa Zulane de Oliveira Fernando da Conceição Aline Silva Luiz Alberto Rossi Maria Santos Juliana da Andrioli João |
| author_facet | Sousa Zulane de Oliveira Fernando da Conceição Aline Silva Luiz Alberto Rossi Maria Santos Juliana da Andrioli João |
| author_sort | Sousa Zulane |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Chenopodium ambrosioides and Kielmeyera neglecta</it> are plants traditionally used in Brazil to treat various infectious diseases. The study of the biological activities of these plants is of great importance for the detection of biologically active compounds.</p> <p>Methods</p> <p>Extracts from these plants were extracted with hexane (Hex), dichloromethane (DCM), ethyl acetate (EtOAc) and ethanol (EtOH) and assessed for their antimicrobial properties, bioactivity against <it>Artemia salina</it> Leach and antifungal action on the cell wall of <it>Neurospora crassa</it>.</p> <p>Results</p> <p>Extracts from <it>C. ambrosioides</it> (Hex, DCM and EtOH) and <it>K. neglecta</it> (EtOAc and EtOH) showed high bioactivity against <it>A. salina</it> (LD50 < 1000 μg/mL), which might be associated with cytotoxic activity against cancer cells. <it>C. ambrosioides</it> Hex and DCM showed specific activity against yeasts, highlighting the activity of hexanic extract against <it>Candida krusei</it> (MIC = 100 μg/mL). By comparing the inhibitory concentration of 50% growth (IC 50%) with the growth control, extracts from <it>K. neglecta</it> EtOAc and EtOH have shown activities against multidrug-resistant bacteria (<it>Enterococcus faecalis</it> ATCC 51299 and <it>Staphylococcus aureus</it> ATCC 43300), with IC 50% of 12.5 μg/mL The assay carried out on <it>N. crassa</it> allowed defining that extracts with antifungal activity do not have action through inhibition of cell wall synthesis.</p> <p>Conclusions</p> <p>Generally speaking, extracts from <it>C. ambrosioides</it> and <it>K. neglecta</it> showed biological activities that have made the search for bioactive substances in these plants more attractive, illustrating the success of their use in the Brazilian folk medicine.</p> |
| first_indexed | 2024-12-12T04:29:59Z |
| format | Article |
| id | doaj.art-1f8f9d02a6b94ae69550c29e8e0c3cd1 |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-12-12T04:29:59Z |
| publishDate | 2012-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-1f8f9d02a6b94ae69550c29e8e0c3cd12022-12-22T00:38:06ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112012-07-011112010.1186/1476-0711-11-20Biological activities of extracts from <it>Chenopodium ambrosioides</it> Lineu and <it>Kielmeyera neglecta</it> SaddiSousa Zulanede Oliveira Fernandoda Conceição AlineSilva Luiz AlbertoRossi MariaSantos Juliana daAndrioli João<p>Abstract</p> <p>Background</p> <p><it>Chenopodium ambrosioides and Kielmeyera neglecta</it> are plants traditionally used in Brazil to treat various infectious diseases. The study of the biological activities of these plants is of great importance for the detection of biologically active compounds.</p> <p>Methods</p> <p>Extracts from these plants were extracted with hexane (Hex), dichloromethane (DCM), ethyl acetate (EtOAc) and ethanol (EtOH) and assessed for their antimicrobial properties, bioactivity against <it>Artemia salina</it> Leach and antifungal action on the cell wall of <it>Neurospora crassa</it>.</p> <p>Results</p> <p>Extracts from <it>C. ambrosioides</it> (Hex, DCM and EtOH) and <it>K. neglecta</it> (EtOAc and EtOH) showed high bioactivity against <it>A. salina</it> (LD50 < 1000 μg/mL), which might be associated with cytotoxic activity against cancer cells. <it>C. ambrosioides</it> Hex and DCM showed specific activity against yeasts, highlighting the activity of hexanic extract against <it>Candida krusei</it> (MIC = 100 μg/mL). By comparing the inhibitory concentration of 50% growth (IC 50%) with the growth control, extracts from <it>K. neglecta</it> EtOAc and EtOH have shown activities against multidrug-resistant bacteria (<it>Enterococcus faecalis</it> ATCC 51299 and <it>Staphylococcus aureus</it> ATCC 43300), with IC 50% of 12.5 μg/mL The assay carried out on <it>N. crassa</it> allowed defining that extracts with antifungal activity do not have action through inhibition of cell wall synthesis.</p> <p>Conclusions</p> <p>Generally speaking, extracts from <it>C. ambrosioides</it> and <it>K. neglecta</it> showed biological activities that have made the search for bioactive substances in these plants more attractive, illustrating the success of their use in the Brazilian folk medicine.</p>http://www.ann-clinmicrob.com/content/11/1/20AntimicrobialBioactivityExtract<it>Chenopodium ambrosioides</it><it>Kielmeyera neglecta</it><it>Candida</it> |
| spellingShingle | Sousa Zulane de Oliveira Fernando da Conceição Aline Silva Luiz Alberto Rossi Maria Santos Juliana da Andrioli João Biological activities of extracts from <it>Chenopodium ambrosioides</it> Lineu and <it>Kielmeyera neglecta</it> Saddi Annals of Clinical Microbiology and Antimicrobials Antimicrobial Bioactivity Extract <it>Chenopodium ambrosioides</it> <it>Kielmeyera neglecta</it> <it>Candida</it> |
| title | Biological activities of extracts from <it>Chenopodium ambrosioides</it> Lineu and <it>Kielmeyera neglecta</it> Saddi |
| title_full | Biological activities of extracts from <it>Chenopodium ambrosioides</it> Lineu and <it>Kielmeyera neglecta</it> Saddi |
| title_fullStr | Biological activities of extracts from <it>Chenopodium ambrosioides</it> Lineu and <it>Kielmeyera neglecta</it> Saddi |
| title_full_unstemmed | Biological activities of extracts from <it>Chenopodium ambrosioides</it> Lineu and <it>Kielmeyera neglecta</it> Saddi |
| title_short | Biological activities of extracts from <it>Chenopodium ambrosioides</it> Lineu and <it>Kielmeyera neglecta</it> Saddi |
| title_sort | biological activities of extracts from it chenopodium ambrosioides it lineu and it kielmeyera neglecta it saddi |
| topic | Antimicrobial Bioactivity Extract <it>Chenopodium ambrosioides</it> <it>Kielmeyera neglecta</it> <it>Candida</it> |
| url | http://www.ann-clinmicrob.com/content/11/1/20 |
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