High Performance Liquid Chromatography-Size Exclusion Chromatography (Hplc-Sec) As an Efficient Tool for The Quantification of Polymers
Poly (lactide-co-glycolide acid) ( PLGA) is an extraordinary well-described polymer and has excellent pharmaceutical properties like high biocompatibility and good biodegradability. Hence, it is one of the most used materials for drug delivery and biomedical systems, also being present in several US...
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| Format: | Article |
| Language: | English |
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PURKH
2019-12-01
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| Series: | To Chemistry Journal |
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| Online Access: | https://purkh.com/index.php/tochem/article/view/550 |
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| author | Shripad Deepak D. Kayande Mazahar Farooqui Parag Deshmukh |
| author_facet | Shripad Deepak D. Kayande Mazahar Farooqui Parag Deshmukh |
| author_sort | Shripad |
| collection | DOAJ |
| description | Poly (lactide-co-glycolide acid) ( PLGA) is an extraordinary well-described polymer and has excellent pharmaceutical properties like high biocompatibility and good biodegradability. Hence, it is one of the most used materials for drug delivery and biomedical systems, also being present in several US Food and Drug Administration approved carrier systems and therapeutic devices. For both applications, the quantification of polymer is important. During the development of the production process, parameters like yield or loading efficacy are essential to be determined. Although PLGA is a well-defined biomaterial, it still lacks a sensitive and convenient quantification approach for PLGA-based systems. Thus, we present a new method for fast and precise quantification of PLGA by HPLC-SEC. The method includes a shorter run time of 20 minutes with a size exclusion column of 300mm x 8.0mm diameter, tetrahydrofuran as mobile phase and diluent, the detection was carried out using the refractive index detector. The developed method has a detection limit of 0.1 ppm, enabling the quantification of low amounts of PLGA. Compared to existing approaches, like gravimetric or nuclear magnetic resonance measurements, which are tedious or expensive, the developed method is fast, ideal for routine screening and it is selective since no interference. The developed method is validated in terms of selectivity, precision, linearity, accuracy and solution stability. Due to the high sensitivity and rapidity of the method, it is suitable for both, laboratory and industrial use |
| first_indexed | 2024-12-11T02:42:06Z |
| format | Article |
| id | doaj.art-1f9057406ef241bfa65f2a42bda0653a |
| institution | Directory Open Access Journal |
| issn | 2581-7507 |
| language | English |
| last_indexed | 2024-12-11T02:42:06Z |
| publishDate | 2019-12-01 |
| publisher | PURKH |
| record_format | Article |
| series | To Chemistry Journal |
| spelling | doaj.art-1f9057406ef241bfa65f2a42bda0653a2022-12-22T01:23:31ZengPURKHTo Chemistry Journal2581-75072019-12-014550High Performance Liquid Chromatography-Size Exclusion Chromatography (Hplc-Sec) As an Efficient Tool for The Quantification of PolymersShripad0Deepak D. Kayande1Mazahar Farooqui2Parag Deshmukh3S.B.E.S. College, Aurangabad, MaharashtraS.B.E.S. College of scienceMaulana Azad CollegeICICI knowledge park ShameerpetPoly (lactide-co-glycolide acid) ( PLGA) is an extraordinary well-described polymer and has excellent pharmaceutical properties like high biocompatibility and good biodegradability. Hence, it is one of the most used materials for drug delivery and biomedical systems, also being present in several US Food and Drug Administration approved carrier systems and therapeutic devices. For both applications, the quantification of polymer is important. During the development of the production process, parameters like yield or loading efficacy are essential to be determined. Although PLGA is a well-defined biomaterial, it still lacks a sensitive and convenient quantification approach for PLGA-based systems. Thus, we present a new method for fast and precise quantification of PLGA by HPLC-SEC. The method includes a shorter run time of 20 minutes with a size exclusion column of 300mm x 8.0mm diameter, tetrahydrofuran as mobile phase and diluent, the detection was carried out using the refractive index detector. The developed method has a detection limit of 0.1 ppm, enabling the quantification of low amounts of PLGA. Compared to existing approaches, like gravimetric or nuclear magnetic resonance measurements, which are tedious or expensive, the developed method is fast, ideal for routine screening and it is selective since no interference. The developed method is validated in terms of selectivity, precision, linearity, accuracy and solution stability. Due to the high sensitivity and rapidity of the method, it is suitable for both, laboratory and industrial usehttps://purkh.com/index.php/tochem/article/view/550Poly (Lactide-Co-Glycolide AcidPLGAQuantificationHPLC-SECRefractive Index |
| spellingShingle | Shripad Deepak D. Kayande Mazahar Farooqui Parag Deshmukh High Performance Liquid Chromatography-Size Exclusion Chromatography (Hplc-Sec) As an Efficient Tool for The Quantification of Polymers To Chemistry Journal Poly (Lactide-Co-Glycolide Acid PLGA Quantification HPLC-SEC Refractive Index |
| title | High Performance Liquid Chromatography-Size Exclusion Chromatography (Hplc-Sec) As an Efficient Tool for The Quantification of Polymers |
| title_full | High Performance Liquid Chromatography-Size Exclusion Chromatography (Hplc-Sec) As an Efficient Tool for The Quantification of Polymers |
| title_fullStr | High Performance Liquid Chromatography-Size Exclusion Chromatography (Hplc-Sec) As an Efficient Tool for The Quantification of Polymers |
| title_full_unstemmed | High Performance Liquid Chromatography-Size Exclusion Chromatography (Hplc-Sec) As an Efficient Tool for The Quantification of Polymers |
| title_short | High Performance Liquid Chromatography-Size Exclusion Chromatography (Hplc-Sec) As an Efficient Tool for The Quantification of Polymers |
| title_sort | high performance liquid chromatography size exclusion chromatography hplc sec as an efficient tool for the quantification of polymers |
| topic | Poly (Lactide-Co-Glycolide Acid PLGA Quantification HPLC-SEC Refractive Index |
| url | https://purkh.com/index.php/tochem/article/view/550 |
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