Associations between Maternal Diet, Body Composition and Gut Microbial Ecology in Pregnancy
Maternal body composition, gestational weight gain (GWG) and diet quality influence offspring obesity risk. While the gut microbiome is thought to play a crucial role, it is understudied in pregnancy. Using a longitudinal pregnancy cohort, maternal anthropometrics, body composition, fecal microbiome...
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MDPI AG
2021-09-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/9/3295 |
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| author | Meghan L. Ruebel Stephanie P. Gilley Clark R. Sims Ying Zhong Donald Turner Sree V. Chintapalli Brian D. Piccolo Aline Andres Kartik Shankar |
| author_facet | Meghan L. Ruebel Stephanie P. Gilley Clark R. Sims Ying Zhong Donald Turner Sree V. Chintapalli Brian D. Piccolo Aline Andres Kartik Shankar |
| author_sort | Meghan L. Ruebel |
| collection | DOAJ |
| description | Maternal body composition, gestational weight gain (GWG) and diet quality influence offspring obesity risk. While the gut microbiome is thought to play a crucial role, it is understudied in pregnancy. Using a longitudinal pregnancy cohort, maternal anthropometrics, body composition, fecal microbiome and dietary intake were assessed at 12, 24 and 36 weeks of gestation. Fecal samples (<i>n</i> = 101, 98 and 107, at each trimester, respectively) were utilized for microbiome analysis via 16S rRNA amplicon sequencing. Data analysis included alpha- and beta-diversity measures and assessment of compositional changes using <i>MaAsLin2</i>. Correlation analyses of serum metabolic and anthropometric markers were performed against bacterial abundance and predicted functional pathways. α-diversity was unaltered by pregnancy stage or maternal obesity status. Actinobacteria, <i>Lachnospiraceae</i>, <i>Akkermansia</i>, <i>Bifidobacterium</i>, <i>Streptococcus</i> and <i>Anaerotuncus</i> abundances were associated with gestation stage. Maternal obesity status was associated with increased abundance <i>of Lachnospiraceae</i>, <i>Bilophila</i>, <i>Dialister</i> and <i>Roseburia</i>. Maternal BMI, fat mass, triglyceride and insulin levels were positively associated with <i>Bilophila</i>. Correlations of bacterial abundance with diet intake showed that <i>Ruminococcus</i> and <i>Paraprevotella</i> were associated with total fat and unsaturated fatty acid intake, while <i>Collinsella</i> and <i>Anaerostipes</i> were associated with protein intake. While causal relationships remain unclear, collectively, these findings indicate pregnancy- and maternal obesity-dependent interactions between dietary factors and the maternal gut microbiome. |
| first_indexed | 2024-03-10T07:20:00Z |
| format | Article |
| id | doaj.art-1f914236d88d4920a10b8ed52c0af8ea |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T07:20:00Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-1f914236d88d4920a10b8ed52c0af8ea2023-11-22T14:40:29ZengMDPI AGNutrients2072-66432021-09-01139329510.3390/nu13093295Associations between Maternal Diet, Body Composition and Gut Microbial Ecology in PregnancyMeghan L. Ruebel0Stephanie P. Gilley1Clark R. Sims2Ying Zhong3Donald Turner4Sree V. Chintapalli5Brian D. Piccolo6Aline Andres7Kartik Shankar8Department of Pediatrics, Section of Nutrition, Anschutz Medical Campus, School of Medicine, University of Colorado, Aurora, CO 80045, USADepartment of Pediatrics, Section of Nutrition, Anschutz Medical Campus, School of Medicine, University of Colorado, Aurora, CO 80045, USAArkansas Children’s Nutrition Center, Little Rock, AR 72202, USAArkansas Children’s Nutrition Center, Little Rock, AR 72202, USAArkansas Children’s Nutrition Center, Little Rock, AR 72202, USAArkansas Children’s Nutrition Center, Little Rock, AR 72202, USAArkansas Children’s Nutrition Center, Little Rock, AR 72202, USAArkansas Children’s Nutrition Center, Little Rock, AR 72202, USADepartment of Pediatrics, Section of Nutrition, Anschutz Medical Campus, School of Medicine, University of Colorado, Aurora, CO 80045, USAMaternal body composition, gestational weight gain (GWG) and diet quality influence offspring obesity risk. While the gut microbiome is thought to play a crucial role, it is understudied in pregnancy. Using a longitudinal pregnancy cohort, maternal anthropometrics, body composition, fecal microbiome and dietary intake were assessed at 12, 24 and 36 weeks of gestation. Fecal samples (<i>n</i> = 101, 98 and 107, at each trimester, respectively) were utilized for microbiome analysis via 16S rRNA amplicon sequencing. Data analysis included alpha- and beta-diversity measures and assessment of compositional changes using <i>MaAsLin2</i>. Correlation analyses of serum metabolic and anthropometric markers were performed against bacterial abundance and predicted functional pathways. α-diversity was unaltered by pregnancy stage or maternal obesity status. Actinobacteria, <i>Lachnospiraceae</i>, <i>Akkermansia</i>, <i>Bifidobacterium</i>, <i>Streptococcus</i> and <i>Anaerotuncus</i> abundances were associated with gestation stage. Maternal obesity status was associated with increased abundance <i>of Lachnospiraceae</i>, <i>Bilophila</i>, <i>Dialister</i> and <i>Roseburia</i>. Maternal BMI, fat mass, triglyceride and insulin levels were positively associated with <i>Bilophila</i>. Correlations of bacterial abundance with diet intake showed that <i>Ruminococcus</i> and <i>Paraprevotella</i> were associated with total fat and unsaturated fatty acid intake, while <i>Collinsella</i> and <i>Anaerostipes</i> were associated with protein intake. While causal relationships remain unclear, collectively, these findings indicate pregnancy- and maternal obesity-dependent interactions between dietary factors and the maternal gut microbiome.https://www.mdpi.com/2072-6643/13/9/3295dietmicrobiomeobesitypregnancyadipositymetabolic syndrome |
| spellingShingle | Meghan L. Ruebel Stephanie P. Gilley Clark R. Sims Ying Zhong Donald Turner Sree V. Chintapalli Brian D. Piccolo Aline Andres Kartik Shankar Associations between Maternal Diet, Body Composition and Gut Microbial Ecology in Pregnancy Nutrients diet microbiome obesity pregnancy adiposity metabolic syndrome |
| title | Associations between Maternal Diet, Body Composition and Gut Microbial Ecology in Pregnancy |
| title_full | Associations between Maternal Diet, Body Composition and Gut Microbial Ecology in Pregnancy |
| title_fullStr | Associations between Maternal Diet, Body Composition and Gut Microbial Ecology in Pregnancy |
| title_full_unstemmed | Associations between Maternal Diet, Body Composition and Gut Microbial Ecology in Pregnancy |
| title_short | Associations between Maternal Diet, Body Composition and Gut Microbial Ecology in Pregnancy |
| title_sort | associations between maternal diet body composition and gut microbial ecology in pregnancy |
| topic | diet microbiome obesity pregnancy adiposity metabolic syndrome |
| url | https://www.mdpi.com/2072-6643/13/9/3295 |
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