Biofilm Formation and Antibiotic Resistance Phenotype of <i>Helicobacter pylori</i> Clinical Isolates
We evaluated biofilm formation of clinical <i>Helicobacter pylori</i> isolates from Indonesia and its relation to antibiotic resistance. We determined the minimum inhibition concentration (MIC) of amoxicillin, clarithromycin, levofloxacin, metronidazole and tetracycline by the Etest to m...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/12/8/473 |
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| author | Kartika Afrida Fauzia Muhammad Miftahussurur Ari Fahrial Syam Langgeng Agung Waskito Dalla Doohan Yudith Annisa Ayu Rezkitha Takashi Matsumoto Vo Phuoc Tuan Junko Akada Hideo Yonezawa Shigeru Kamiya Yoshio Yamaoka |
| author_facet | Kartika Afrida Fauzia Muhammad Miftahussurur Ari Fahrial Syam Langgeng Agung Waskito Dalla Doohan Yudith Annisa Ayu Rezkitha Takashi Matsumoto Vo Phuoc Tuan Junko Akada Hideo Yonezawa Shigeru Kamiya Yoshio Yamaoka |
| author_sort | Kartika Afrida Fauzia |
| collection | DOAJ |
| description | We evaluated biofilm formation of clinical <i>Helicobacter pylori</i> isolates from Indonesia and its relation to antibiotic resistance. We determined the minimum inhibition concentration (MIC) of amoxicillin, clarithromycin, levofloxacin, metronidazole and tetracycline by the Etest to measure the planktonic susceptibility of 101 <i>H. pylori</i> strains. Biofilms were quantified by the crystal violet method. The minimum biofilm eradication concentration (MBEC) was obtained by measuring the survival of bacteria in a biofilm after exposure to antibiotics. The majority of the strains formed a biofilm (93.1% (94/101)), including weak (75.5%) and strong (24.5%) biofilm-formers. Planktonic resistant and sensitive strains produced relatively equal amounts of biofilms. The resistance proportion, shown by the MBEC measurement, was higher in the strong biofilm group for all antibiotics compared to the weak biofilm group, especially for clarithromycin (<i>p =</i> 0.002). Several cases showed sensitivity by the MIC measurement, but resistance according to the MBEC measurements (amoxicillin, 47.6%; tetracycline, 57.1%; clarithromycin, 19.0%; levofloxacin, 38.1%; and metronidazole 38.1%). Thus, biofilm formation may increase the survival of <i>H. pylori</i> and its resistance to antibiotics. Biofilm-related antibiotic resistance should be evaluated with antibiotic susceptibility. |
| first_indexed | 2024-03-10T18:13:43Z |
| format | Article |
| id | doaj.art-1f93871f164142f0bdd30867664dbb63 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T18:13:43Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-1f93871f164142f0bdd30867664dbb632023-11-20T07:53:11ZengMDPI AGToxins2072-66512020-07-0112847310.3390/toxins12080473Biofilm Formation and Antibiotic Resistance Phenotype of <i>Helicobacter pylori</i> Clinical IsolatesKartika Afrida Fauzia0Muhammad Miftahussurur1Ari Fahrial Syam2Langgeng Agung Waskito3Dalla Doohan4Yudith Annisa Ayu Rezkitha5Takashi Matsumoto6Vo Phuoc Tuan7Junko Akada8Hideo Yonezawa9Shigeru Kamiya10Yoshio Yamaoka11Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu 879-5593, JapanInstitute of Tropical Disease, Universitas Airlangga, Surabaya 60115, IndonesiaDivision of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, University of Indonesia, Jakarta 10430, IndonesiaInstitute of Tropical Disease, Universitas Airlangga, Surabaya 60115, IndonesiaDepartment of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu 879-5593, JapanInstitute of Tropical Disease, Universitas Airlangga, Surabaya 60115, IndonesiaDepartment of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu 879-5593, JapanDepartment of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu 879-5593, JapanDepartment of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu 879-5593, JapanDepartment of Infectious Diseases, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, JapanDepartment of Infectious Diseases, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, JapanDepartment of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu 879-5593, JapanWe evaluated biofilm formation of clinical <i>Helicobacter pylori</i> isolates from Indonesia and its relation to antibiotic resistance. We determined the minimum inhibition concentration (MIC) of amoxicillin, clarithromycin, levofloxacin, metronidazole and tetracycline by the Etest to measure the planktonic susceptibility of 101 <i>H. pylori</i> strains. Biofilms were quantified by the crystal violet method. The minimum biofilm eradication concentration (MBEC) was obtained by measuring the survival of bacteria in a biofilm after exposure to antibiotics. The majority of the strains formed a biofilm (93.1% (94/101)), including weak (75.5%) and strong (24.5%) biofilm-formers. Planktonic resistant and sensitive strains produced relatively equal amounts of biofilms. The resistance proportion, shown by the MBEC measurement, was higher in the strong biofilm group for all antibiotics compared to the weak biofilm group, especially for clarithromycin (<i>p =</i> 0.002). Several cases showed sensitivity by the MIC measurement, but resistance according to the MBEC measurements (amoxicillin, 47.6%; tetracycline, 57.1%; clarithromycin, 19.0%; levofloxacin, 38.1%; and metronidazole 38.1%). Thus, biofilm formation may increase the survival of <i>H. pylori</i> and its resistance to antibiotics. Biofilm-related antibiotic resistance should be evaluated with antibiotic susceptibility.https://www.mdpi.com/2072-6651/12/8/473<i>Helicobacter pylori</i>antibiotic resistancebiofilm formationbiofilm-specific resistanceamoxicillinclarithromycin |
| spellingShingle | Kartika Afrida Fauzia Muhammad Miftahussurur Ari Fahrial Syam Langgeng Agung Waskito Dalla Doohan Yudith Annisa Ayu Rezkitha Takashi Matsumoto Vo Phuoc Tuan Junko Akada Hideo Yonezawa Shigeru Kamiya Yoshio Yamaoka Biofilm Formation and Antibiotic Resistance Phenotype of <i>Helicobacter pylori</i> Clinical Isolates Toxins <i>Helicobacter pylori</i> antibiotic resistance biofilm formation biofilm-specific resistance amoxicillin clarithromycin |
| title | Biofilm Formation and Antibiotic Resistance Phenotype of <i>Helicobacter pylori</i> Clinical Isolates |
| title_full | Biofilm Formation and Antibiotic Resistance Phenotype of <i>Helicobacter pylori</i> Clinical Isolates |
| title_fullStr | Biofilm Formation and Antibiotic Resistance Phenotype of <i>Helicobacter pylori</i> Clinical Isolates |
| title_full_unstemmed | Biofilm Formation and Antibiotic Resistance Phenotype of <i>Helicobacter pylori</i> Clinical Isolates |
| title_short | Biofilm Formation and Antibiotic Resistance Phenotype of <i>Helicobacter pylori</i> Clinical Isolates |
| title_sort | biofilm formation and antibiotic resistance phenotype of i helicobacter pylori i clinical isolates |
| topic | <i>Helicobacter pylori</i> antibiotic resistance biofilm formation biofilm-specific resistance amoxicillin clarithromycin |
| url | https://www.mdpi.com/2072-6651/12/8/473 |
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