Biosynthesis, Characterization, Antibacterial and Synergistic Effect of Silver Nanoparticles using Fusarium oxysporum
Biosynthesis of silver nanoparticles was achieved using cell filtrate from submerged fermentation of Fusarium oxysporum. It was found that AgNO3 reduced to Ag nanoparticles when exposed to the cell filtrate and the colour of solution was dark brown with absorbance peak at 430 nm wavelength. TEM micr...
| Príomhchruthaitheoirí: | , , , |
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| Formáid: | Alt |
| Teanga: | English |
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Journal of Pure and Applied Microbiology
2017-09-01
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| Sraith: | Journal of Pure and Applied Microbiology |
| Ábhair: | |
| Rochtain ar líne: | https://microbiologyjournal.org/biosynthesis-characterization-antibacterial-and-synergistic-effect-of-silver-nanoparticles-using-fusariumoxysporum/ |
| _version_ | 1830175040186875904 |
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| author | Ehab B. El Domany Tamer M. Essam Amr E. Ahmed A.A. Farghali |
| author_facet | Ehab B. El Domany Tamer M. Essam Amr E. Ahmed A.A. Farghali |
| author_sort | Ehab B. El Domany |
| collection | DOAJ |
| description | Biosynthesis of silver nanoparticles was achieved using cell filtrate from submerged fermentation of Fusarium oxysporum. It was found that AgNO3 reduced to Ag nanoparticles when exposed to the cell filtrate and the colour of solution was dark brown with absorbance peak at 430 nm wavelength. TEM micrograph showed spherical AgNPs with range 10-25 nm in dimension and was well dispersed. AgNPs show high stability in solution due to biological stabilizing and capping agents released from fungus, and have negative charge -25mv.Biosynthesized AgNPs have high potential antibacterial and antifungal activity, highest inhibitory zone was (27) mm against Candida albicans. The synergistic effect of AgNPs gave highest fold increase (10) against E. coli, followed by (5) fold against Staphylococcus auras using Azithromycin and levofloxacin as standard antibiotics respectively. |
| first_indexed | 2024-12-17T18:45:01Z |
| format | Article |
| id | doaj.art-1f94e863818c41a3b3c4437b6c6e3d04 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-17T18:45:01Z |
| publishDate | 2017-09-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-1f94e863818c41a3b3c4437b6c6e3d042022-12-21T21:36:48ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2017-09-011131441144610.22207/JPAM.11.3.27Biosynthesis, Characterization, Antibacterial and Synergistic Effect of Silver Nanoparticles using Fusarium oxysporumEhab B. El Domany0Tamer M. Essam1Amr E. Ahmed2A.A. Farghali3Biotechnology and life Science Department, Faculty of Postgraduate Studies for Advanced Science, Beni-Suef University, Egypt.Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Kasr Al-Aini Street, Cairo 11562, Egypt.Biotechnology and life Science Department, Faculty of Postgraduate Studies for Advanced Science, Beni-Suef University, Egypt.Material Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Science, Beni-Suef University, Egypt.Biosynthesis of silver nanoparticles was achieved using cell filtrate from submerged fermentation of Fusarium oxysporum. It was found that AgNO3 reduced to Ag nanoparticles when exposed to the cell filtrate and the colour of solution was dark brown with absorbance peak at 430 nm wavelength. TEM micrograph showed spherical AgNPs with range 10-25 nm in dimension and was well dispersed. AgNPs show high stability in solution due to biological stabilizing and capping agents released from fungus, and have negative charge -25mv.Biosynthesized AgNPs have high potential antibacterial and antifungal activity, highest inhibitory zone was (27) mm against Candida albicans. The synergistic effect of AgNPs gave highest fold increase (10) against E. coli, followed by (5) fold against Staphylococcus auras using Azithromycin and levofloxacin as standard antibiotics respectively.https://microbiologyjournal.org/biosynthesis-characterization-antibacterial-and-synergistic-effect-of-silver-nanoparticles-using-fusariumoxysporum/biosynthesissilver nanoparticlesantibacterial |
| spellingShingle | Ehab B. El Domany Tamer M. Essam Amr E. Ahmed A.A. Farghali Biosynthesis, Characterization, Antibacterial and Synergistic Effect of Silver Nanoparticles using Fusarium oxysporum Journal of Pure and Applied Microbiology biosynthesis silver nanoparticles antibacterial |
| title | Biosynthesis, Characterization, Antibacterial and Synergistic Effect of Silver Nanoparticles using Fusarium oxysporum |
| title_full | Biosynthesis, Characterization, Antibacterial and Synergistic Effect of Silver Nanoparticles using Fusarium oxysporum |
| title_fullStr | Biosynthesis, Characterization, Antibacterial and Synergistic Effect of Silver Nanoparticles using Fusarium oxysporum |
| title_full_unstemmed | Biosynthesis, Characterization, Antibacterial and Synergistic Effect of Silver Nanoparticles using Fusarium oxysporum |
| title_short | Biosynthesis, Characterization, Antibacterial and Synergistic Effect of Silver Nanoparticles using Fusarium oxysporum |
| title_sort | biosynthesis characterization antibacterial and synergistic effect of silver nanoparticles using fusarium oxysporum |
| topic | biosynthesis silver nanoparticles antibacterial |
| url | https://microbiologyjournal.org/biosynthesis-characterization-antibacterial-and-synergistic-effect-of-silver-nanoparticles-using-fusariumoxysporum/ |
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