Respiratory syncytial virus genotypes NA1, ON1, and BA9 are prevalent in Thailand, 2012–2015
Respiratory syncytial virus (RSV) causes acute lower respiratory tract infection in infants and young children worldwide. To investigate the RSV burden in Thailand over four consecutive years (January 2012 to December 2015), we screened 3,306 samples obtained from children ≤5 years old with acute re...
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PeerJ Inc.
2017-10-01
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| author | Ilada Thongpan John Mauleekoonphairoj Preeyaporn Vichiwattana Sumeth Korkong Rujipat Wasitthankasem Sompong Vongpunsawad Yong Poovorawan |
| author_facet | Ilada Thongpan John Mauleekoonphairoj Preeyaporn Vichiwattana Sumeth Korkong Rujipat Wasitthankasem Sompong Vongpunsawad Yong Poovorawan |
| author_sort | Ilada Thongpan |
| collection | DOAJ |
| description | Respiratory syncytial virus (RSV) causes acute lower respiratory tract infection in infants and young children worldwide. To investigate the RSV burden in Thailand over four consecutive years (January 2012 to December 2015), we screened 3,306 samples obtained from children ≤5 years old with acute respiratory tract infection using semi-nested reverse-transcription polymerase chain reaction (RT-PCR). In all, 8.4% (277/3,306) of the specimens tested positive for RSV, most of which appeared in the rainy months of July to November. We then genotyped RSV by sequencing the G glycoprotein gene and performed phylogenetic analysis to determine the RSV antigenic subgroup. The majority (57.4%, 159/277) of the RSV belonged to subgroup A (RSV-A), of which NA1 genotype was the most common in 2012 while ON1 genotype became prevalent the following year. Among samples tested positive for RSV-B subgroup B (RSV-B) (42.6%, 118/277), most were genotype BA9 (92.6%, 87/94) with some BA10 and BA-C. Predicted amino acid sequence from the partial G region showed highly conserved N-linked glycosylation site at residue N237 among all RSV-A ON1 strains (68/68), and at residues N296 (86/87) and N310 (87/87) among RSV-B BA9 strains. Positive selection of key residues combined with notable sequence variations on the G gene contributed to the continued circulation of this rapidly evolving virus. |
| first_indexed | 2024-03-09T06:38:57Z |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:38:57Z |
| publishDate | 2017-10-01 |
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| spelling | doaj.art-1f98b3950da942cdaff66ecfb7af8c632023-12-03T10:52:59ZengPeerJ Inc.PeerJ2167-83592017-10-015e397010.7717/peerj.3970Respiratory syncytial virus genotypes NA1, ON1, and BA9 are prevalent in Thailand, 2012–2015Ilada Thongpan0John Mauleekoonphairoj1Preeyaporn Vichiwattana2Sumeth Korkong3Rujipat Wasitthankasem4Sompong Vongpunsawad5Yong Poovorawan6Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, ThailandCenter of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, ThailandCenter of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, ThailandCenter of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, ThailandCenter of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, ThailandCenter of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, ThailandCenter of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, ThailandRespiratory syncytial virus (RSV) causes acute lower respiratory tract infection in infants and young children worldwide. To investigate the RSV burden in Thailand over four consecutive years (January 2012 to December 2015), we screened 3,306 samples obtained from children ≤5 years old with acute respiratory tract infection using semi-nested reverse-transcription polymerase chain reaction (RT-PCR). In all, 8.4% (277/3,306) of the specimens tested positive for RSV, most of which appeared in the rainy months of July to November. We then genotyped RSV by sequencing the G glycoprotein gene and performed phylogenetic analysis to determine the RSV antigenic subgroup. The majority (57.4%, 159/277) of the RSV belonged to subgroup A (RSV-A), of which NA1 genotype was the most common in 2012 while ON1 genotype became prevalent the following year. Among samples tested positive for RSV-B subgroup B (RSV-B) (42.6%, 118/277), most were genotype BA9 (92.6%, 87/94) with some BA10 and BA-C. Predicted amino acid sequence from the partial G region showed highly conserved N-linked glycosylation site at residue N237 among all RSV-A ON1 strains (68/68), and at residues N296 (86/87) and N310 (87/87) among RSV-B BA9 strains. Positive selection of key residues combined with notable sequence variations on the G gene contributed to the continued circulation of this rapidly evolving virus.https://peerj.com/articles/3970.pdfPrevalenceRespiratory syncytial virusThailandG glycoprotein |
| spellingShingle | Ilada Thongpan John Mauleekoonphairoj Preeyaporn Vichiwattana Sumeth Korkong Rujipat Wasitthankasem Sompong Vongpunsawad Yong Poovorawan Respiratory syncytial virus genotypes NA1, ON1, and BA9 are prevalent in Thailand, 2012–2015 PeerJ Prevalence Respiratory syncytial virus Thailand G glycoprotein |
| title | Respiratory syncytial virus genotypes NA1, ON1, and BA9 are prevalent in Thailand, 2012–2015 |
| title_full | Respiratory syncytial virus genotypes NA1, ON1, and BA9 are prevalent in Thailand, 2012–2015 |
| title_fullStr | Respiratory syncytial virus genotypes NA1, ON1, and BA9 are prevalent in Thailand, 2012–2015 |
| title_full_unstemmed | Respiratory syncytial virus genotypes NA1, ON1, and BA9 are prevalent in Thailand, 2012–2015 |
| title_short | Respiratory syncytial virus genotypes NA1, ON1, and BA9 are prevalent in Thailand, 2012–2015 |
| title_sort | respiratory syncytial virus genotypes na1 on1 and ba9 are prevalent in thailand 2012 2015 |
| topic | Prevalence Respiratory syncytial virus Thailand G glycoprotein |
| url | https://peerj.com/articles/3970.pdf |
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