Inhibition of Influenza Virus Infection by Lentinus edodes Mycelia Extract Through Its Direct Action and Immunopotentiating Activity
Lentinula edodes mycelia (LEM) solid culture extracts contain many bioactive compounds with diverse pharmacological activities such as antitumor, antiviral, and immunopotentiating effects. In this study, we examined the anti-influenza virus activity of LEM in vitro and in vivo. LEM directly inhibite...
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| Format: | Article |
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Frontiers Media S.A.
2018-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01164/full |
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| author | Takahiro Kuroki Sangjoon Lee Mikako Hirohama Tomohiro Taku Michiko Kumakura Takahiro Haruyama Kyosuke Nagata Atsushi Kawaguchi Atsushi Kawaguchi Atsushi Kawaguchi |
| author_facet | Takahiro Kuroki Sangjoon Lee Mikako Hirohama Tomohiro Taku Michiko Kumakura Takahiro Haruyama Kyosuke Nagata Atsushi Kawaguchi Atsushi Kawaguchi Atsushi Kawaguchi |
| author_sort | Takahiro Kuroki |
| collection | DOAJ |
| description | Lentinula edodes mycelia (LEM) solid culture extracts contain many bioactive compounds with diverse pharmacological activities such as antitumor, antiviral, and immunopotentiating effects. In this study, we examined the anti-influenza virus activity of LEM in vitro and in vivo. LEM directly inhibited influenza virus growth in vitro at early phases of infection, possibly at the entry process of viral particles to host cells. We also found that the nasal administration of LEM increased the survival rate of infected mice, and this was likely due to the direct action of LEM on the viral growth. The oral administration of LEM showed prolonged median survival time of infected mice. Histological analysis revealed that the moderate bronchiolitis was observed in infected mice by the oral administration with LEM, and the extent of alveolitis was dramatically reduced. The orally LEM-administered mice showed a rapid activation of IFN-β gene expression upon influenza virus infection. These results suggest that the immunopotentiation activity of LEM on type I IFN pathway represses the virus spread to distal alveolar regions from peribronchiolar regions which are primary infection sites in the mouse model. We propose that LEM has anti-influenza virus activities through the direct action on viral growth and stimulatory activity of innate immunity. |
| first_indexed | 2024-12-10T14:15:15Z |
| format | Article |
| id | doaj.art-1f99bd44c57043caa8dc737788e87a3c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T14:15:15Z |
| publishDate | 2018-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1f99bd44c57043caa8dc737788e87a3c2022-12-22T01:45:22ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-05-01910.3389/fmicb.2018.01164360631Inhibition of Influenza Virus Infection by Lentinus edodes Mycelia Extract Through Its Direct Action and Immunopotentiating ActivityTakahiro Kuroki0Sangjoon Lee1Mikako Hirohama2Tomohiro Taku3Michiko Kumakura4Takahiro Haruyama5Kyosuke Nagata6Atsushi Kawaguchi7Atsushi Kawaguchi8Atsushi Kawaguchi9Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, JapanPh.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Tsukuba, JapanDepartment of Infection Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, JapanGraduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, JapanGraduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, JapanDepartment of Infection Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, JapanDepartment of Infection Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, JapanGraduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, JapanPh.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Tsukuba, JapanDepartment of Infection Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, JapanLentinula edodes mycelia (LEM) solid culture extracts contain many bioactive compounds with diverse pharmacological activities such as antitumor, antiviral, and immunopotentiating effects. In this study, we examined the anti-influenza virus activity of LEM in vitro and in vivo. LEM directly inhibited influenza virus growth in vitro at early phases of infection, possibly at the entry process of viral particles to host cells. We also found that the nasal administration of LEM increased the survival rate of infected mice, and this was likely due to the direct action of LEM on the viral growth. The oral administration of LEM showed prolonged median survival time of infected mice. Histological analysis revealed that the moderate bronchiolitis was observed in infected mice by the oral administration with LEM, and the extent of alveolitis was dramatically reduced. The orally LEM-administered mice showed a rapid activation of IFN-β gene expression upon influenza virus infection. These results suggest that the immunopotentiation activity of LEM on type I IFN pathway represses the virus spread to distal alveolar regions from peribronchiolar regions which are primary infection sites in the mouse model. We propose that LEM has anti-influenza virus activities through the direct action on viral growth and stimulatory activity of innate immunity.https://www.frontiersin.org/article/10.3389/fmicb.2018.01164/fullLEMinflammationinfluenza virusinterferonpneumonia |
| spellingShingle | Takahiro Kuroki Sangjoon Lee Mikako Hirohama Tomohiro Taku Michiko Kumakura Takahiro Haruyama Kyosuke Nagata Atsushi Kawaguchi Atsushi Kawaguchi Atsushi Kawaguchi Inhibition of Influenza Virus Infection by Lentinus edodes Mycelia Extract Through Its Direct Action and Immunopotentiating Activity Frontiers in Microbiology LEM inflammation influenza virus interferon pneumonia |
| title | Inhibition of Influenza Virus Infection by Lentinus edodes Mycelia Extract Through Its Direct Action and Immunopotentiating Activity |
| title_full | Inhibition of Influenza Virus Infection by Lentinus edodes Mycelia Extract Through Its Direct Action and Immunopotentiating Activity |
| title_fullStr | Inhibition of Influenza Virus Infection by Lentinus edodes Mycelia Extract Through Its Direct Action and Immunopotentiating Activity |
| title_full_unstemmed | Inhibition of Influenza Virus Infection by Lentinus edodes Mycelia Extract Through Its Direct Action and Immunopotentiating Activity |
| title_short | Inhibition of Influenza Virus Infection by Lentinus edodes Mycelia Extract Through Its Direct Action and Immunopotentiating Activity |
| title_sort | inhibition of influenza virus infection by lentinus edodes mycelia extract through its direct action and immunopotentiating activity |
| topic | LEM inflammation influenza virus interferon pneumonia |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01164/full |
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