Molecular analyses detect natural coinfection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) in serologically negative animals
Infection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) has been confirmed in several studies by serological and molecular techniques. In order to determine the presence of persistently infected animals and circulating species and subtypes of BVDV we conducted this s...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier España
2015-06-01
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| Series: | Revista Argentina de Microbiología |
| Subjects: | |
| Online Access: | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412015000200012&lng=en&tlng=en |
| _version_ | 1819128399902277632 |
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| author | María I Craig Guido A König Daniel F Benitez María G Draghi |
| author_facet | María I Craig Guido A König Daniel F Benitez María G Draghi |
| author_sort | María I Craig |
| collection | DOAJ |
| description | Infection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) has been confirmed in several studies by serological and molecular techniques. In order to determine the presence of persistently infected animals and circulating species and subtypes of BVDV we conducted this study on a buffalo herd, whose habitat was shared with bovine cattle (Bossp.). Our serological results showed a high level of positivity for BVDV-1 and BVDV-2 within the buffalo herd. The molecular analyses of blood samples in serologically negative animals revealed the presence of viral nucleic acid, confirming the existence of persistent infection in the buffaloes. Cloning and sequencing of the 5′ UTR of some of these samples revealed the presence of naturally mix-infected buffaloes with at least two different subtypes (1a and 1b), and also with both BVDV species (BVDV-1 and BVDV-2). |
| first_indexed | 2024-12-22T08:27:13Z |
| format | Article |
| id | doaj.art-1f9a688e681c4570a78881872aecd80b |
| institution | Directory Open Access Journal |
| issn | 1851-7617 |
| language | English |
| last_indexed | 2024-12-22T08:27:13Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier España |
| record_format | Article |
| series | Revista Argentina de Microbiología |
| spelling | doaj.art-1f9a688e681c4570a78881872aecd80b2022-12-21T18:32:36ZengElsevier EspañaRevista Argentina de Microbiología1851-76172015-06-0147214815110.1016/j.ram.2015.03.001S0325-75412015000200012Molecular analyses detect natural coinfection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) in serologically negative animalsMaría I Craig0Guido A König1Daniel F Benitez2María G Draghi3Instituto Nacional de Tecnología AgropecuariaInstituto Nacional de Tecnología AgropecuariaINTAINTAInfection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) has been confirmed in several studies by serological and molecular techniques. In order to determine the presence of persistently infected animals and circulating species and subtypes of BVDV we conducted this study on a buffalo herd, whose habitat was shared with bovine cattle (Bossp.). Our serological results showed a high level of positivity for BVDV-1 and BVDV-2 within the buffalo herd. The molecular analyses of blood samples in serologically negative animals revealed the presence of viral nucleic acid, confirming the existence of persistent infection in the buffaloes. Cloning and sequencing of the 5′ UTR of some of these samples revealed the presence of naturally mix-infected buffaloes with at least two different subtypes (1a and 1b), and also with both BVDV species (BVDV-1 and BVDV-2).http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412015000200012&lng=en&tlng=enBúfaloVirus de la diarrea viral bovinaInfección persistenteCoinfección |
| spellingShingle | María I Craig Guido A König Daniel F Benitez María G Draghi Molecular analyses detect natural coinfection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) in serologically negative animals Revista Argentina de Microbiología Búfalo Virus de la diarrea viral bovina Infección persistente Coinfección |
| title | Molecular analyses detect natural coinfection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) in serologically negative animals |
| title_full | Molecular analyses detect natural coinfection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) in serologically negative animals |
| title_fullStr | Molecular analyses detect natural coinfection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) in serologically negative animals |
| title_full_unstemmed | Molecular analyses detect natural coinfection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) in serologically negative animals |
| title_short | Molecular analyses detect natural coinfection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) in serologically negative animals |
| title_sort | molecular analyses detect natural coinfection of water buffaloes bubalus bubalis with bovine viral diarrhea viruses bvdv in serologically negative animals |
| topic | Búfalo Virus de la diarrea viral bovina Infección persistente Coinfección |
| url | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412015000200012&lng=en&tlng=en |
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