Comparative Proteomic Analysis Unveils Critical Pathways Underlying the Role of Nitrogen Fertilizer Treatment in American Elderberry
American elderberry (<i>Sambucus nigra</i> subsp. <i>canadensis</i>) is a rapidly growing specialty crop in Missouri and eastern North America. Nitrogen (N) is a major nutrient involved in plant growth and development. However, proteome changes for different genotypes of elde...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-03-01
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| Series: | Proteomes |
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| Online Access: | https://www.mdpi.com/2227-7382/7/1/10 |
| _version_ | 1811298330517438464 |
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| author | Bo Yang Andrew L. Thomas C. Michael Greenlief |
| author_facet | Bo Yang Andrew L. Thomas C. Michael Greenlief |
| author_sort | Bo Yang |
| collection | DOAJ |
| description | American elderberry (<i>Sambucus nigra</i> subsp. <i>canadensis</i>) is a rapidly growing specialty crop in Missouri and eastern North America. Nitrogen (N) is a major nutrient involved in plant growth and development. However, proteome changes for different genotypes of elder in response to varying levels of N-treatment remain undefined. To reveal plant responses to N, comparative proteomic analyses were performed to determine consistent changes in three genotypes of elderberry leaves (Adams II, Bob Gordon and Wyldewood) grown under different N-fertilizer treatments. 165 proteins separated by two dimensional gel electrophoresis showed significant differences in abundance (<i>p</i> < 0.05 and greater than 2-fold). Principal component analysis of the abundance profiles of these proteins revealed Bob Gordon as a distinct genotype. The 165 proteins were identified by mass spectrometry and showed similar functional distributions in these genotypes underlying the N-treatment. Among the proteins identified, 23 are mainly involved in photosynthesis, protein metabolism and redox homeostasis. Their abundance profiles were not altered upon exposure to N or genotype. These results provide novel insights into plant responses to fertilizer treatment at the proteome level and could lead to a better understanding of molecular mechanisms of elderberry growth. |
| first_indexed | 2024-04-13T06:18:44Z |
| format | Article |
| id | doaj.art-1f9b2ea3d8794c4098a7715f005f7310 |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-04-13T06:18:44Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-1f9b2ea3d8794c4098a7715f005f73102022-12-22T02:58:44ZengMDPI AGProteomes2227-73822019-03-01711010.3390/proteomes7010010proteomes7010010Comparative Proteomic Analysis Unveils Critical Pathways Underlying the Role of Nitrogen Fertilizer Treatment in American ElderberryBo Yang0Andrew L. Thomas1C. Michael Greenlief2Department of Chemistry, University of Missouri, Columbia, MO 65211, USADivision of Plant Sciences, Southwest Research Center, University of Missouri, Mt. Vernon, MO 65712, USADepartment of Chemistry, University of Missouri, Columbia, MO 65211, USAAmerican elderberry (<i>Sambucus nigra</i> subsp. <i>canadensis</i>) is a rapidly growing specialty crop in Missouri and eastern North America. Nitrogen (N) is a major nutrient involved in plant growth and development. However, proteome changes for different genotypes of elder in response to varying levels of N-treatment remain undefined. To reveal plant responses to N, comparative proteomic analyses were performed to determine consistent changes in three genotypes of elderberry leaves (Adams II, Bob Gordon and Wyldewood) grown under different N-fertilizer treatments. 165 proteins separated by two dimensional gel electrophoresis showed significant differences in abundance (<i>p</i> < 0.05 and greater than 2-fold). Principal component analysis of the abundance profiles of these proteins revealed Bob Gordon as a distinct genotype. The 165 proteins were identified by mass spectrometry and showed similar functional distributions in these genotypes underlying the N-treatment. Among the proteins identified, 23 are mainly involved in photosynthesis, protein metabolism and redox homeostasis. Their abundance profiles were not altered upon exposure to N or genotype. These results provide novel insights into plant responses to fertilizer treatment at the proteome level and could lead to a better understanding of molecular mechanisms of elderberry growth.https://www.mdpi.com/2227-7382/7/1/10elderberrycomparative proteomicsnitrogen response<i>Sambucus</i> |
| spellingShingle | Bo Yang Andrew L. Thomas C. Michael Greenlief Comparative Proteomic Analysis Unveils Critical Pathways Underlying the Role of Nitrogen Fertilizer Treatment in American Elderberry Proteomes elderberry comparative proteomics nitrogen response <i>Sambucus</i> |
| title | Comparative Proteomic Analysis Unveils Critical Pathways Underlying the Role of Nitrogen Fertilizer Treatment in American Elderberry |
| title_full | Comparative Proteomic Analysis Unveils Critical Pathways Underlying the Role of Nitrogen Fertilizer Treatment in American Elderberry |
| title_fullStr | Comparative Proteomic Analysis Unveils Critical Pathways Underlying the Role of Nitrogen Fertilizer Treatment in American Elderberry |
| title_full_unstemmed | Comparative Proteomic Analysis Unveils Critical Pathways Underlying the Role of Nitrogen Fertilizer Treatment in American Elderberry |
| title_short | Comparative Proteomic Analysis Unveils Critical Pathways Underlying the Role of Nitrogen Fertilizer Treatment in American Elderberry |
| title_sort | comparative proteomic analysis unveils critical pathways underlying the role of nitrogen fertilizer treatment in american elderberry |
| topic | elderberry comparative proteomics nitrogen response <i>Sambucus</i> |
| url | https://www.mdpi.com/2227-7382/7/1/10 |
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