The Association of MEG3 lncRNA with Nuclear Speckles in Living Cells
Nuclear speckles are nuclear bodies containing RNA-binding proteins as well as RNAs including long non-coding RNAs (lncRNAs). Maternally expressed gene 3 (MEG3) is a nuclear retained lncRNA found to associate with nuclear speckles. To understand the association dynamics of MEG3 lncRNA with nuclear s...
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MDPI AG
2022-06-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/12/1942 |
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| author | Sarah E. Hasenson Ella Alkalay Mohammad K. Atrash Alon Boocholez Julianna Gershbaum Hodaya Hochberg-Laufer Yaron Shav-Tal |
| author_facet | Sarah E. Hasenson Ella Alkalay Mohammad K. Atrash Alon Boocholez Julianna Gershbaum Hodaya Hochberg-Laufer Yaron Shav-Tal |
| author_sort | Sarah E. Hasenson |
| collection | DOAJ |
| description | Nuclear speckles are nuclear bodies containing RNA-binding proteins as well as RNAs including long non-coding RNAs (lncRNAs). Maternally expressed gene 3 (MEG3) is a nuclear retained lncRNA found to associate with nuclear speckles. To understand the association dynamics of MEG3 lncRNA with nuclear speckles in living cells, we generated a fluorescently tagged MEG3 transcript that could be detected in real time. Under regular conditions, transient association of MEG3 with nuclear speckles was observed, including a nucleoplasmic fraction. Transcription or splicing inactivation conditions, known to affect nuclear speckle structure, showed prominent and increased association of MEG3 lncRNA with the nuclear speckles, specifically forming a ring-like structure around the nuclear speckles. This contrasted with metastasis-associated lung adenocarcinoma (MALAT1) lncRNA that is normally highly associated with nuclear speckles, which was released and dispersed in the nucleoplasm. Under normal conditions, MEG3 dynamically associated with the periphery of the nuclear speckles, but under transcription or splicing inhibition, MEG3 could also enter the center of the nuclear speckle. Altogether, using live-cell imaging approaches, we find that MEG3 lncRNA is a transient resident of nuclear speckles and that its association with this nuclear body is modulated by the levels of transcription and splicing activities in the cell. |
| first_indexed | 2024-03-10T00:09:50Z |
| format | Article |
| id | doaj.art-1f9c75d9e8c049789e9af9c07a61deaf |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T00:09:50Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-1f9c75d9e8c049789e9af9c07a61deaf2023-11-23T16:01:56ZengMDPI AGCells2073-44092022-06-011112194210.3390/cells11121942The Association of MEG3 lncRNA with Nuclear Speckles in Living CellsSarah E. Hasenson0Ella Alkalay1Mohammad K. Atrash2Alon Boocholez3Julianna Gershbaum4Hodaya Hochberg-Laufer5Yaron Shav-Tal6The Mina & Everard Goodman Faculty of Life Sciences and The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, IsraelThe Mina & Everard Goodman Faculty of Life Sciences and The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, IsraelThe Mina & Everard Goodman Faculty of Life Sciences and The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, IsraelThe Mina & Everard Goodman Faculty of Life Sciences and The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, IsraelThe Mina & Everard Goodman Faculty of Life Sciences and The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, IsraelThe Mina & Everard Goodman Faculty of Life Sciences and The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, IsraelThe Mina & Everard Goodman Faculty of Life Sciences and The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, IsraelNuclear speckles are nuclear bodies containing RNA-binding proteins as well as RNAs including long non-coding RNAs (lncRNAs). Maternally expressed gene 3 (MEG3) is a nuclear retained lncRNA found to associate with nuclear speckles. To understand the association dynamics of MEG3 lncRNA with nuclear speckles in living cells, we generated a fluorescently tagged MEG3 transcript that could be detected in real time. Under regular conditions, transient association of MEG3 with nuclear speckles was observed, including a nucleoplasmic fraction. Transcription or splicing inactivation conditions, known to affect nuclear speckle structure, showed prominent and increased association of MEG3 lncRNA with the nuclear speckles, specifically forming a ring-like structure around the nuclear speckles. This contrasted with metastasis-associated lung adenocarcinoma (MALAT1) lncRNA that is normally highly associated with nuclear speckles, which was released and dispersed in the nucleoplasm. Under normal conditions, MEG3 dynamically associated with the periphery of the nuclear speckles, but under transcription or splicing inhibition, MEG3 could also enter the center of the nuclear speckle. Altogether, using live-cell imaging approaches, we find that MEG3 lncRNA is a transient resident of nuclear speckles and that its association with this nuclear body is modulated by the levels of transcription and splicing activities in the cell.https://www.mdpi.com/2073-4409/11/12/1942lncRNAMEG3nuclear bodiesnuclear specklesnucleussplicing |
| spellingShingle | Sarah E. Hasenson Ella Alkalay Mohammad K. Atrash Alon Boocholez Julianna Gershbaum Hodaya Hochberg-Laufer Yaron Shav-Tal The Association of MEG3 lncRNA with Nuclear Speckles in Living Cells Cells lncRNA MEG3 nuclear bodies nuclear speckles nucleus splicing |
| title | The Association of MEG3 lncRNA with Nuclear Speckles in Living Cells |
| title_full | The Association of MEG3 lncRNA with Nuclear Speckles in Living Cells |
| title_fullStr | The Association of MEG3 lncRNA with Nuclear Speckles in Living Cells |
| title_full_unstemmed | The Association of MEG3 lncRNA with Nuclear Speckles in Living Cells |
| title_short | The Association of MEG3 lncRNA with Nuclear Speckles in Living Cells |
| title_sort | association of meg3 lncrna with nuclear speckles in living cells |
| topic | lncRNA MEG3 nuclear bodies nuclear speckles nucleus splicing |
| url | https://www.mdpi.com/2073-4409/11/12/1942 |
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