Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito, <i>Aedes aegypti</i>
Although the role of exogenous small interfering RNA (siRNA) and P-element induced wimpy testis (PIWI)-interacting RNA (piRNA) pathways in mosquito antiviral immunity is increasingly better understood, there is still little knowledge regarding the role of mosquito cellular microRNA (miRNA). Identify...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/11/6/540 |
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| author | Pei-Shi Yen Chun-Hong Chen Vattipally Sreenu Alain Kohl Anna-Bella Failloux |
| author_facet | Pei-Shi Yen Chun-Hong Chen Vattipally Sreenu Alain Kohl Anna-Bella Failloux |
| author_sort | Pei-Shi Yen |
| collection | DOAJ |
| description | Although the role of exogenous small interfering RNA (siRNA) and P-element induced wimpy testis (PIWI)-interacting RNA (piRNA) pathways in mosquito antiviral immunity is increasingly better understood, there is still little knowledge regarding the role of mosquito cellular microRNA (miRNA). Identifying direct interactions between the mosquito miRNAs and the RNA genome of arboviruses and choosing the relevant miRNA candidates to explore resulting antiviral mechanisms are critical. Here, we carried out genomic analyses to identify <i>Aedes aegypti</i> miRNAs that potentially interact with various lineages and genotypes of chikungunya, dengue, and Zika viruses. By using prediction tools with distinct algorithms, several miRNA binding sites were commonly found within different genotypes/and or lineages of each arbovirus. We further analyzed those miRNAs that could target more than one arbovirus, required a low energy threshold to form miRNA-viralRNA (vRNA) complexes, and predicted potential RNA structures using RNAhybrid software. We predicted miRNA candidates that might participate in regulating arboviral replication in <i>Ae. aegypti</i>. Even without any experimental validation, which should be done as a next step, this study can shed further light on the role of miRNA in mosquito innate immunity and targets for future studies. |
| first_indexed | 2024-12-11T01:54:29Z |
| format | Article |
| id | doaj.art-1f9d04219029444986dfed063bac8f44 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-11T01:54:29Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-1f9d04219029444986dfed063bac8f442022-12-22T01:24:40ZengMDPI AGViruses1999-49152019-06-0111654010.3390/v11060540v11060540Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito, <i>Aedes aegypti</i>Pei-Shi Yen0Chun-Hong Chen1Vattipally Sreenu2Alain Kohl3Anna-Bella Failloux4Unit of Arboviruses and Insect Vectors, Department of Virology, Institut Pasteur, 75015 Paris, FranceNational Health Research Institutes, National Institute of Infectious Diseases and Vaccinology, Miaoli 35053, TaiwanMRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, Scotland, UKMRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, Scotland, UKUnit of Arboviruses and Insect Vectors, Department of Virology, Institut Pasteur, 75015 Paris, FranceAlthough the role of exogenous small interfering RNA (siRNA) and P-element induced wimpy testis (PIWI)-interacting RNA (piRNA) pathways in mosquito antiviral immunity is increasingly better understood, there is still little knowledge regarding the role of mosquito cellular microRNA (miRNA). Identifying direct interactions between the mosquito miRNAs and the RNA genome of arboviruses and choosing the relevant miRNA candidates to explore resulting antiviral mechanisms are critical. Here, we carried out genomic analyses to identify <i>Aedes aegypti</i> miRNAs that potentially interact with various lineages and genotypes of chikungunya, dengue, and Zika viruses. By using prediction tools with distinct algorithms, several miRNA binding sites were commonly found within different genotypes/and or lineages of each arbovirus. We further analyzed those miRNAs that could target more than one arbovirus, required a low energy threshold to form miRNA-viralRNA (vRNA) complexes, and predicted potential RNA structures using RNAhybrid software. We predicted miRNA candidates that might participate in regulating arboviral replication in <i>Ae. aegypti</i>. Even without any experimental validation, which should be done as a next step, this study can shed further light on the role of miRNA in mosquito innate immunity and targets for future studies.https://www.mdpi.com/1999-4915/11/6/540<i>Aedes aegypti</i>arboviruseschikungunyadengueZikamiRNA |
| spellingShingle | Pei-Shi Yen Chun-Hong Chen Vattipally Sreenu Alain Kohl Anna-Bella Failloux Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito, <i>Aedes aegypti</i> Viruses <i>Aedes aegypti</i> arboviruses chikungunya dengue Zika miRNA |
| title | Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito, <i>Aedes aegypti</i> |
| title_full | Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito, <i>Aedes aegypti</i> |
| title_fullStr | Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito, <i>Aedes aegypti</i> |
| title_full_unstemmed | Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito, <i>Aedes aegypti</i> |
| title_short | Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito, <i>Aedes aegypti</i> |
| title_sort | assessing the potential interactions between cellular mirna and arboviral genomic rna in the yellow fever mosquito i aedes aegypti i |
| topic | <i>Aedes aegypti</i> arboviruses chikungunya dengue Zika miRNA |
| url | https://www.mdpi.com/1999-4915/11/6/540 |
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