Investigating the Effect of Basic Amino Acids and Glucosamine on the Solubility of Ibuprofen and Piroxicam
Purpose: Poor aqueous solubility hampers the development of several compounds as pharmacological agents. Hence, preparing novel formulations with augmented absorption is a challenge in pharmaceutical industries. In this paper, we have examined the effect of basic amino acids including arginine (ARG)...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2023-07-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | https://apb.tbzmed.ac.ir/PDF/apb-13-532.pdf |
| _version_ | 1797673420491587584 |
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| author | Hadi Valizadeh Somayeh Mahdinloo Negin Zakeri Muhammad Sarfraz Saeed Nezafat Parvin Zakeri-Milani |
| author_facet | Hadi Valizadeh Somayeh Mahdinloo Negin Zakeri Muhammad Sarfraz Saeed Nezafat Parvin Zakeri-Milani |
| author_sort | Hadi Valizadeh |
| collection | DOAJ |
| description | Purpose: Poor aqueous solubility hampers the development of several compounds as pharmacological agents. Hence, preparing novel formulations with augmented absorption is a challenge in pharmaceutical industries. In this paper, we have examined the effect of basic amino acids including arginine (ARG), lysine (LYS), and glucosamine (GlucN) on the solubility of ibuprofen (IBU) and piroxicam (PXM) as drugs with limited solubility. We have also studied the effect of the dissolution media with the pH values 1.2 to 7.4. Methods: The saturation shake-flask method was used for solubility studies in the presence of amino acids. Briefly, buffer solutions containing different concentrations of amino acids were prepared. Then, an excess amount of each drug with these buffers was shaken to reach equilibrium. After 48 hours, the upper phase was separated, and solubility was calculated by reading their UV-Vis absorbance. Results: The results illustrated that amino acids increased solubility of both drugs with different ratios, which were pH and concentration-dependent. Solubility improved as the amount of amino acids went up, and this upward pattern was more robust with ARG than LYS. The presence of GlucN in citrate buffer significantly enhanced IBU solubility. The solubility of PXM in accompany of GlucN in water did not change significantly while in citrate buffer solubility enhanced specially at pH 6. Conclusion: Overall, GlucN in citrate buffer and ARG in phosphate buffer could be introduced as the most suitable media for IBU and PXM solubility improvement, respectively. |
| first_indexed | 2024-03-11T21:44:18Z |
| format | Article |
| id | doaj.art-1f9e1b0d578840dd9a3bbca6d12bc422 |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-03-11T21:44:18Z |
| publishDate | 2023-07-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-1f9e1b0d578840dd9a3bbca6d12bc4222023-09-26T10:43:48ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082023-07-0113353253810.34172/apb.2023.067apb-37878Investigating the Effect of Basic Amino Acids and Glucosamine on the Solubility of Ibuprofen and PiroxicamHadi Valizadeh0Somayeh Mahdinloo1Negin Zakeri2Muhammad Sarfraz3Saeed Nezafat4Parvin Zakeri-Milani5Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.College of Pharmacy, Al Ain University, Al Ain 64141, United Arab Emirates.Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.Liver and Gastrointestinal Diseases Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.Purpose: Poor aqueous solubility hampers the development of several compounds as pharmacological agents. Hence, preparing novel formulations with augmented absorption is a challenge in pharmaceutical industries. In this paper, we have examined the effect of basic amino acids including arginine (ARG), lysine (LYS), and glucosamine (GlucN) on the solubility of ibuprofen (IBU) and piroxicam (PXM) as drugs with limited solubility. We have also studied the effect of the dissolution media with the pH values 1.2 to 7.4. Methods: The saturation shake-flask method was used for solubility studies in the presence of amino acids. Briefly, buffer solutions containing different concentrations of amino acids were prepared. Then, an excess amount of each drug with these buffers was shaken to reach equilibrium. After 48 hours, the upper phase was separated, and solubility was calculated by reading their UV-Vis absorbance. Results: The results illustrated that amino acids increased solubility of both drugs with different ratios, which were pH and concentration-dependent. Solubility improved as the amount of amino acids went up, and this upward pattern was more robust with ARG than LYS. The presence of GlucN in citrate buffer significantly enhanced IBU solubility. The solubility of PXM in accompany of GlucN in water did not change significantly while in citrate buffer solubility enhanced specially at pH 6. Conclusion: Overall, GlucN in citrate buffer and ARG in phosphate buffer could be introduced as the most suitable media for IBU and PXM solubility improvement, respectively.https://apb.tbzmed.ac.ir/PDF/apb-13-532.pdfsolubilityibuprofenpiroxicamargininelysineglucosamine |
| spellingShingle | Hadi Valizadeh Somayeh Mahdinloo Negin Zakeri Muhammad Sarfraz Saeed Nezafat Parvin Zakeri-Milani Investigating the Effect of Basic Amino Acids and Glucosamine on the Solubility of Ibuprofen and Piroxicam Advanced Pharmaceutical Bulletin solubility ibuprofen piroxicam arginine lysine glucosamine |
| title | Investigating the Effect of Basic Amino Acids and Glucosamine on the Solubility of Ibuprofen and Piroxicam |
| title_full | Investigating the Effect of Basic Amino Acids and Glucosamine on the Solubility of Ibuprofen and Piroxicam |
| title_fullStr | Investigating the Effect of Basic Amino Acids and Glucosamine on the Solubility of Ibuprofen and Piroxicam |
| title_full_unstemmed | Investigating the Effect of Basic Amino Acids and Glucosamine on the Solubility of Ibuprofen and Piroxicam |
| title_short | Investigating the Effect of Basic Amino Acids and Glucosamine on the Solubility of Ibuprofen and Piroxicam |
| title_sort | investigating the effect of basic amino acids and glucosamine on the solubility of ibuprofen and piroxicam |
| topic | solubility ibuprofen piroxicam arginine lysine glucosamine |
| url | https://apb.tbzmed.ac.ir/PDF/apb-13-532.pdf |
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