TRIB3 Promotes the Malignant Progression of Bladder Cancer: An Integrated Analysis of Bioinformatics and in vitro Experiments
BackgroundBladder cancer is a common malignant tumor characterized by high mortality and high management costs; however, it lacks useful molecular prognostic markers. Tribbles pseudokinase 3 (TRIB3) is a pseudokinase that participates in cell tumor progression and metabolism and whose function in bl...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.649208/full |
| _version_ | 1818620586750902272 |
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| author | Jieping Yang Jiaxing Lin Jun An Yongkang Zhao Siyang Jing Meng Yu Yuyan Zhu Yang Yao |
| author_facet | Jieping Yang Jiaxing Lin Jun An Yongkang Zhao Siyang Jing Meng Yu Yuyan Zhu Yang Yao |
| author_sort | Jieping Yang |
| collection | DOAJ |
| description | BackgroundBladder cancer is a common malignant tumor characterized by high mortality and high management costs; however, it lacks useful molecular prognostic markers. Tribbles pseudokinase 3 (TRIB3) is a pseudokinase that participates in cell tumor progression and metabolism and whose function in bladder cancer is not precisely known.Main MethodsWe downloaded transcriptome data and clinical data of bladder cancer from associated databases and extracted the expression matrix of TRIB3 for multiple bioinformatics analysis. RT-PCR detected the expression of TRIB3 in bladder cancer cells. After knockdown of TRIB3 with siRNA, we investigated TRIB3 function using CCK8, Cell Cycle and Transwell assays.Key FindingsKaplan–Meier analysis of TRIB3 in the four cohorts showed that high expression of TRIB3 correlated with poor outcome. Expression of TRIB3 positively correlated with stage and grade and down-regulation of TRIB3 expression significantly inhibited proliferation, migration and cell cycle of bladder cancer cells.SignificanceTRIB3 is a potential prognostic marker and therapeutic target. It can be used to individualize the treatment of bladder cancer. |
| first_indexed | 2024-12-16T17:55:44Z |
| format | Article |
| id | doaj.art-1f9fc9b0dc894ccd962c0af49f9f60f1 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-16T17:55:44Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-1f9fc9b0dc894ccd962c0af49f9f60f12022-12-21T22:22:10ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-03-011210.3389/fgene.2021.649208649208TRIB3 Promotes the Malignant Progression of Bladder Cancer: An Integrated Analysis of Bioinformatics and in vitro ExperimentsJieping Yang0Jiaxing Lin1Jun An2Yongkang Zhao3Siyang Jing4Meng Yu5Yuyan Zhu6Yang Yao7Department of Urology, The First Hospital of China Medical University, Shenyang, ChinaDepartment of Urology, The First Hospital of China Medical University, Shenyang, ChinaDepartment of Urology, Shenzhen Second People’s Hospital, Shenzhen, ChinaJoint Laboratory of Artificial Intelligence and Precision Medicine of China Medical University and Northeastern University, Northeastern University, Shenyang, ChinaDepartment of Laboratory Animal Science, China Medical University, Shenyang, ChinaDepartment of Laboratory Animal Science, China Medical University, Shenyang, ChinaDepartment of Urology, The First Hospital of China Medical University, Shenyang, ChinaDepartment of Physiology, Shenyang Medical College, Shenyang, ChinaBackgroundBladder cancer is a common malignant tumor characterized by high mortality and high management costs; however, it lacks useful molecular prognostic markers. Tribbles pseudokinase 3 (TRIB3) is a pseudokinase that participates in cell tumor progression and metabolism and whose function in bladder cancer is not precisely known.Main MethodsWe downloaded transcriptome data and clinical data of bladder cancer from associated databases and extracted the expression matrix of TRIB3 for multiple bioinformatics analysis. RT-PCR detected the expression of TRIB3 in bladder cancer cells. After knockdown of TRIB3 with siRNA, we investigated TRIB3 function using CCK8, Cell Cycle and Transwell assays.Key FindingsKaplan–Meier analysis of TRIB3 in the four cohorts showed that high expression of TRIB3 correlated with poor outcome. Expression of TRIB3 positively correlated with stage and grade and down-regulation of TRIB3 expression significantly inhibited proliferation, migration and cell cycle of bladder cancer cells.SignificanceTRIB3 is a potential prognostic marker and therapeutic target. It can be used to individualize the treatment of bladder cancer.https://www.frontiersin.org/articles/10.3389/fgene.2021.649208/fullTRIB3bladder cancerbiomarkerproliferatemetastasis |
| spellingShingle | Jieping Yang Jiaxing Lin Jun An Yongkang Zhao Siyang Jing Meng Yu Yuyan Zhu Yang Yao TRIB3 Promotes the Malignant Progression of Bladder Cancer: An Integrated Analysis of Bioinformatics and in vitro Experiments Frontiers in Genetics TRIB3 bladder cancer biomarker proliferate metastasis |
| title | TRIB3 Promotes the Malignant Progression of Bladder Cancer: An Integrated Analysis of Bioinformatics and in vitro Experiments |
| title_full | TRIB3 Promotes the Malignant Progression of Bladder Cancer: An Integrated Analysis of Bioinformatics and in vitro Experiments |
| title_fullStr | TRIB3 Promotes the Malignant Progression of Bladder Cancer: An Integrated Analysis of Bioinformatics and in vitro Experiments |
| title_full_unstemmed | TRIB3 Promotes the Malignant Progression of Bladder Cancer: An Integrated Analysis of Bioinformatics and in vitro Experiments |
| title_short | TRIB3 Promotes the Malignant Progression of Bladder Cancer: An Integrated Analysis of Bioinformatics and in vitro Experiments |
| title_sort | trib3 promotes the malignant progression of bladder cancer an integrated analysis of bioinformatics and in vitro experiments |
| topic | TRIB3 bladder cancer biomarker proliferate metastasis |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.649208/full |
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