Changes in dynamics of tumor/endothelial cell adhesive interactions depending on endothelial cell growth state and elastic properties.
Cancer cell adhesion to the endothelium is a crucial process in hematogenous metastasis, but how the integrity of the endothelial barrier and endothelial cell (EC) mechanical properties influence the adhesion between metastatic cancer cells and the endothelium remain unclear. In the present study, w...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0269552 |
| _version_ | 1797788234619551744 |
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| author | Leike Xie Zhe Sun Nicola J Brown Olga V Glinskii Gerald A Meininger Vladislav V Glinsky |
| author_facet | Leike Xie Zhe Sun Nicola J Brown Olga V Glinskii Gerald A Meininger Vladislav V Glinsky |
| author_sort | Leike Xie |
| collection | DOAJ |
| description | Cancer cell adhesion to the endothelium is a crucial process in hematogenous metastasis, but how the integrity of the endothelial barrier and endothelial cell (EC) mechanical properties influence the adhesion between metastatic cancer cells and the endothelium remain unclear. In the present study, we have measured the adhesion between single cancer cells and two types of ECs at various growth states and their mechanical properties (elasticity) using atomic force microscopy single cell force spectroscopy. We demonstrated that the EC stiffness increased and adhesion with cancer cells decreased, as ECs grew from a single cell to a confluent state and developed cell-cell contacts, but this was reversed when confluent cells returned to a single state in a scratch assay. Our results suggest that the integrity of the endothelial barrier is an important factor in reducing the ability of the metastatic tumor cells to adhere to the vascular endothelium, extravasate and lodge in the vasculature of a distant organ where secondary metastatic tumors would develop. |
| first_indexed | 2024-03-13T01:32:44Z |
| format | Article |
| id | doaj.art-1fa0528f93b946c7a47c7b7969651632 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-13T01:32:44Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1fa0528f93b946c7a47c7b79696516322023-07-04T05:32:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01176e026955210.1371/journal.pone.0269552Changes in dynamics of tumor/endothelial cell adhesive interactions depending on endothelial cell growth state and elastic properties.Leike XieZhe SunNicola J BrownOlga V GlinskiiGerald A MeiningerVladislav V GlinskyCancer cell adhesion to the endothelium is a crucial process in hematogenous metastasis, but how the integrity of the endothelial barrier and endothelial cell (EC) mechanical properties influence the adhesion between metastatic cancer cells and the endothelium remain unclear. In the present study, we have measured the adhesion between single cancer cells and two types of ECs at various growth states and their mechanical properties (elasticity) using atomic force microscopy single cell force spectroscopy. We demonstrated that the EC stiffness increased and adhesion with cancer cells decreased, as ECs grew from a single cell to a confluent state and developed cell-cell contacts, but this was reversed when confluent cells returned to a single state in a scratch assay. Our results suggest that the integrity of the endothelial barrier is an important factor in reducing the ability of the metastatic tumor cells to adhere to the vascular endothelium, extravasate and lodge in the vasculature of a distant organ where secondary metastatic tumors would develop.https://doi.org/10.1371/journal.pone.0269552 |
| spellingShingle | Leike Xie Zhe Sun Nicola J Brown Olga V Glinskii Gerald A Meininger Vladislav V Glinsky Changes in dynamics of tumor/endothelial cell adhesive interactions depending on endothelial cell growth state and elastic properties. PLoS ONE |
| title | Changes in dynamics of tumor/endothelial cell adhesive interactions depending on endothelial cell growth state and elastic properties. |
| title_full | Changes in dynamics of tumor/endothelial cell adhesive interactions depending on endothelial cell growth state and elastic properties. |
| title_fullStr | Changes in dynamics of tumor/endothelial cell adhesive interactions depending on endothelial cell growth state and elastic properties. |
| title_full_unstemmed | Changes in dynamics of tumor/endothelial cell adhesive interactions depending on endothelial cell growth state and elastic properties. |
| title_short | Changes in dynamics of tumor/endothelial cell adhesive interactions depending on endothelial cell growth state and elastic properties. |
| title_sort | changes in dynamics of tumor endothelial cell adhesive interactions depending on endothelial cell growth state and elastic properties |
| url | https://doi.org/10.1371/journal.pone.0269552 |
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