Production of Antibody Raised Against Lipopolysaccharide (LPS) of Vibrio cholerae Non-O1
Background: Cholera, an infectious disease caused by Vibrio cholerae, is primarily transmitted by ingestion of contaminated food or water. In severe cases, cholera may lead to severe dehydration, metabolic acidosis, and ultimately, hypovolemic shock and death. Methods: In this study V.cholerae n...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2008-06-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/2051 |
| _version_ | 1818981079561797632 |
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| author | M Saadati A Godarzi M Akbari MB Salehi H Shirzad M Jahani AA Rahimi |
| author_facet | M Saadati A Godarzi M Akbari MB Salehi H Shirzad M Jahani AA Rahimi |
| author_sort | M Saadati |
| collection | DOAJ |
| description | Background: Cholera, an infectious disease caused by Vibrio cholerae, is primarily transmitted by ingestion of contaminated food or water. In severe cases, cholera may lead to severe dehydration, metabolic acidosis, and ultimately, hypovolemic shock and death.
Methods: In this study V.cholerae non-O1 was cultured in suitable media. LPS was extracted from the surface of bacteria by hot phenol-water method and then purified by high-speed centrifugation. For production of specific antibody against LPS, white newzeland rabbits were first immunized by whole cell bacteria and then immunized with highly purified LPS. The titre of the antiserum was determined by ELISA for each serogroup.
Results: Results presented in this study indicate that serum anti-LPS antibodies raised against purified LPS of V.cholerae non-O1 can detect V.cholerae non-O1.
Conclusion: This antibody had low cross reactivity with V.cholerae O1, serotype Inaba or Ogawa. So, this antibody can be used for for detection of V. cholerae non-O1. |
| first_indexed | 2024-12-20T17:25:37Z |
| format | Article |
| id | doaj.art-1fa076bc85d94e7db4b65f3efa6dfb69 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-20T17:25:37Z |
| publishDate | 2008-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-1fa076bc85d94e7db4b65f3efa6dfb692022-12-21T19:31:36ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932008-06-01372Production of Antibody Raised Against Lipopolysaccharide (LPS) of Vibrio cholerae Non-O1 M Saadati0 A Godarzi1 M Akbari2 MB Salehi3 H Shirzad4 M Jahani5 AA Rahimi6 Background: Cholera, an infectious disease caused by Vibrio cholerae, is primarily transmitted by ingestion of contaminated food or water. In severe cases, cholera may lead to severe dehydration, metabolic acidosis, and ultimately, hypovolemic shock and death. Methods: In this study V.cholerae non-O1 was cultured in suitable media. LPS was extracted from the surface of bacteria by hot phenol-water method and then purified by high-speed centrifugation. For production of specific antibody against LPS, white newzeland rabbits were first immunized by whole cell bacteria and then immunized with highly purified LPS. The titre of the antiserum was determined by ELISA for each serogroup. Results: Results presented in this study indicate that serum anti-LPS antibodies raised against purified LPS of V.cholerae non-O1 can detect V.cholerae non-O1. Conclusion: This antibody had low cross reactivity with V.cholerae O1, serotype Inaba or Ogawa. So, this antibody can be used for for detection of V. cholerae non-O1.https://ijph.tums.ac.ir/index.php/ijph/article/view/2051ImmunoglobulinLipopolysaccharideVibrio cholerae non-O1ELISA |
| spellingShingle | M Saadati A Godarzi M Akbari MB Salehi H Shirzad M Jahani AA Rahimi Production of Antibody Raised Against Lipopolysaccharide (LPS) of Vibrio cholerae Non-O1 Iranian Journal of Public Health Immunoglobulin Lipopolysaccharide Vibrio cholerae non-O1 ELISA |
| title | Production of Antibody Raised Against Lipopolysaccharide (LPS) of Vibrio cholerae Non-O1 |
| title_full | Production of Antibody Raised Against Lipopolysaccharide (LPS) of Vibrio cholerae Non-O1 |
| title_fullStr | Production of Antibody Raised Against Lipopolysaccharide (LPS) of Vibrio cholerae Non-O1 |
| title_full_unstemmed | Production of Antibody Raised Against Lipopolysaccharide (LPS) of Vibrio cholerae Non-O1 |
| title_short | Production of Antibody Raised Against Lipopolysaccharide (LPS) of Vibrio cholerae Non-O1 |
| title_sort | production of antibody raised against lipopolysaccharide lps of vibrio cholerae non o1 |
| topic | Immunoglobulin Lipopolysaccharide Vibrio cholerae non-O1 ELISA |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/2051 |
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