Evolutionary history of the NAM-B1 gene in wild and domesticated tetraploid wheat
Abstract Background The NAM-B1 gene in wheat has for almost three decades been extensively studied and utilized in breeding programs because of its significant impact on grain protein and mineral content and pleiotropic effects on senescence rate and grain size. First detected in wild emmer wheat, t...
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | BMC Genetics |
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| Online Access: | http://link.springer.com/article/10.1186/s12863-017-0566-7 |
| _version_ | 1811336800521682944 |
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| author | Maria Lundström Matti W. Leino Jenny Hagenblad |
| author_facet | Maria Lundström Matti W. Leino Jenny Hagenblad |
| author_sort | Maria Lundström |
| collection | DOAJ |
| description | Abstract Background The NAM-B1 gene in wheat has for almost three decades been extensively studied and utilized in breeding programs because of its significant impact on grain protein and mineral content and pleiotropic effects on senescence rate and grain size. First detected in wild emmer wheat, the wild-type allele of the gene has been introgressed into durum and bread wheat. Later studies have, however, also found the presence of the wild-type allele in some domesticated subspecies. In this study we trace the evolutionary history of the NAM-B1 in tetraploid wheat species and evaluate it as a putative domestication gene. Results Genotyping of wild and landrace tetraploid accessions showed presence of only null alleles in durum. Domesticated emmer wheats contained both null alleles and the wild-type allele while wild emmers, with one exception, only carried the wild-type allele. One of the null alleles consists of a deletion that covers several 100 kb. The other null-allele, a one-basepair frame-shift insertion, likely arose among wild emmer. This allele was the target of a selective sweep, extending over several 100 kb. Conclusions The NAM-B1 gene fulfils some criteria for being a domestication gene by encoding a trait of domestication relevance (seed size) and is here shown to have been under positive selection. The presence of both wild-type and null alleles in domesticated emmer does, however, suggest the gene to be a diversification gene in this species. Further studies of genotype-environment interactions are needed to find out under what conditions selection on different NAM-B1 alleles have been beneficial. |
| first_indexed | 2024-04-13T17:44:51Z |
| format | Article |
| id | doaj.art-1fa266ffb0404b928c716ff30957e3bf |
| institution | Directory Open Access Journal |
| issn | 1471-2156 |
| language | English |
| last_indexed | 2024-04-13T17:44:51Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genetics |
| spelling | doaj.art-1fa266ffb0404b928c716ff30957e3bf2022-12-22T02:37:00ZengBMCBMC Genetics1471-21562017-12-0118111010.1186/s12863-017-0566-7Evolutionary history of the NAM-B1 gene in wild and domesticated tetraploid wheatMaria Lundström0Matti W. Leino1Jenny Hagenblad2Linköping University, IFM BiologyLinköping University, IFM BiologyLinköping University, IFM BiologyAbstract Background The NAM-B1 gene in wheat has for almost three decades been extensively studied and utilized in breeding programs because of its significant impact on grain protein and mineral content and pleiotropic effects on senescence rate and grain size. First detected in wild emmer wheat, the wild-type allele of the gene has been introgressed into durum and bread wheat. Later studies have, however, also found the presence of the wild-type allele in some domesticated subspecies. In this study we trace the evolutionary history of the NAM-B1 in tetraploid wheat species and evaluate it as a putative domestication gene. Results Genotyping of wild and landrace tetraploid accessions showed presence of only null alleles in durum. Domesticated emmer wheats contained both null alleles and the wild-type allele while wild emmers, with one exception, only carried the wild-type allele. One of the null alleles consists of a deletion that covers several 100 kb. The other null-allele, a one-basepair frame-shift insertion, likely arose among wild emmer. This allele was the target of a selective sweep, extending over several 100 kb. Conclusions The NAM-B1 gene fulfils some criteria for being a domestication gene by encoding a trait of domestication relevance (seed size) and is here shown to have been under positive selection. The presence of both wild-type and null alleles in domesticated emmer does, however, suggest the gene to be a diversification gene in this species. Further studies of genotype-environment interactions are needed to find out under what conditions selection on different NAM-B1 alleles have been beneficial.http://link.springer.com/article/10.1186/s12863-017-0566-7Selective sweepGrain protein content (GPC)EmmerDurumDomestication gene |
| spellingShingle | Maria Lundström Matti W. Leino Jenny Hagenblad Evolutionary history of the NAM-B1 gene in wild and domesticated tetraploid wheat BMC Genetics Selective sweep Grain protein content (GPC) Emmer Durum Domestication gene |
| title | Evolutionary history of the NAM-B1 gene in wild and domesticated tetraploid wheat |
| title_full | Evolutionary history of the NAM-B1 gene in wild and domesticated tetraploid wheat |
| title_fullStr | Evolutionary history of the NAM-B1 gene in wild and domesticated tetraploid wheat |
| title_full_unstemmed | Evolutionary history of the NAM-B1 gene in wild and domesticated tetraploid wheat |
| title_short | Evolutionary history of the NAM-B1 gene in wild and domesticated tetraploid wheat |
| title_sort | evolutionary history of the nam b1 gene in wild and domesticated tetraploid wheat |
| topic | Selective sweep Grain protein content (GPC) Emmer Durum Domestication gene |
| url | http://link.springer.com/article/10.1186/s12863-017-0566-7 |
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