A homeopathic remedy from arnica, marigold, St. John’s wort and comfrey accelerates <it>in vitro</it> wound scratch closure of NIH 3T3 fibroblasts
<p>Abstract</p> <p>Background</p> <p>Drugs of plant origin such as <it>Arnica montana</it>, <it>Calendula officinalis</it> or <it>Hypericum perforatum</it> have been frequently used to promote wound healing. While their effect on woun...
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| Format: | Article |
| Language: | English |
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BMC
2012-07-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://www.biomedcentral.com/1472-6882/12/100 |
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| author | Hostanska Katarina Rostock Matthias Melzer Joerg Baumgartner Stephan Saller Reinhard |
| author_facet | Hostanska Katarina Rostock Matthias Melzer Joerg Baumgartner Stephan Saller Reinhard |
| author_sort | Hostanska Katarina |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Drugs of plant origin such as <it>Arnica montana</it>, <it>Calendula officinalis</it> or <it>Hypericum perforatum</it> have been frequently used to promote wound healing. While their effect on wound healing using preparations at pharmacological concentrations was supported by several <it>in vitro</it> and clinical studies, investigations of herbal homeopathic remedies on wound healing process are rare. The objective of this study was to investigate the effect of a commercial low potency homeopathic remedy Similasan® Arnica plus Spray on wound closure in a controlled, blind trial <it>in vitro</it>.</p> <p>Methods</p> <p>We investigated the effect of an ethanolic preparation composed of equal parts of <it>Arnica montana</it> 4x, <it>Calendula officinalis</it> 4x, <it>Hypericum perforatum</it> 4x and <it>Symphytum officinale</it> 6x (0712–2), its succussed hydroalcoholic solvent (0712–1) and unsuccussed solvent (0712–3) on NIH 3T3 fibroblasts. Cell viability was determined by WST-1 assay, cell growth using BrdU uptake, cell migration by chemotaxis assay and wound closure by CytoSelect ™Wound Healing Assay Kit which generated a defined “wound field”. All assays were performed in three independent controlled experiments.</p> <p>Results</p> <p>None of the three substances affected cell viability and none showed a stimulating effect on cell proliferation. Preparation (0712–2) exerted a stimulating effect on fibroblast migration (31.9%) vs 14.7% with succussed solvent (0712–1) at 1:100 dilutions (p < 0.001). Unsuccussed solvent (0712–3) had no influence on cell migration (6.3%; p > 0.05). Preparation (0712–2) at a dilution of 1:100 promoted <it>in vitro</it> wound closure by 59.5% and differed significantly (p < 0.001) from succussed solvent (0712–1), which caused 22.1% wound closure.</p> <p>Conclusion</p> <p>Results of this study showed that the low potency homeopathic remedy (0712–2) exerted <it>in vitro</it> wound closure potential in NIH 3T3 fibroblasts. This effect resulted from stimulation of fibroblasts motility rather than of their mitosis.</p> |
| first_indexed | 2024-12-17T06:12:34Z |
| format | Article |
| id | doaj.art-1fa2a09c1c844b55aa68626338a69e22 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-17T06:12:34Z |
| publishDate | 2012-07-01 |
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| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-1fa2a09c1c844b55aa68626338a69e222022-12-21T22:00:35ZengBMCBMC Complementary and Alternative Medicine1472-68822012-07-0112110010.1186/1472-6882-12-100A homeopathic remedy from arnica, marigold, St. John’s wort and comfrey accelerates <it>in vitro</it> wound scratch closure of NIH 3T3 fibroblastsHostanska KatarinaRostock MatthiasMelzer JoergBaumgartner StephanSaller Reinhard<p>Abstract</p> <p>Background</p> <p>Drugs of plant origin such as <it>Arnica montana</it>, <it>Calendula officinalis</it> or <it>Hypericum perforatum</it> have been frequently used to promote wound healing. While their effect on wound healing using preparations at pharmacological concentrations was supported by several <it>in vitro</it> and clinical studies, investigations of herbal homeopathic remedies on wound healing process are rare. The objective of this study was to investigate the effect of a commercial low potency homeopathic remedy Similasan® Arnica plus Spray on wound closure in a controlled, blind trial <it>in vitro</it>.</p> <p>Methods</p> <p>We investigated the effect of an ethanolic preparation composed of equal parts of <it>Arnica montana</it> 4x, <it>Calendula officinalis</it> 4x, <it>Hypericum perforatum</it> 4x and <it>Symphytum officinale</it> 6x (0712–2), its succussed hydroalcoholic solvent (0712–1) and unsuccussed solvent (0712–3) on NIH 3T3 fibroblasts. Cell viability was determined by WST-1 assay, cell growth using BrdU uptake, cell migration by chemotaxis assay and wound closure by CytoSelect ™Wound Healing Assay Kit which generated a defined “wound field”. All assays were performed in three independent controlled experiments.</p> <p>Results</p> <p>None of the three substances affected cell viability and none showed a stimulating effect on cell proliferation. Preparation (0712–2) exerted a stimulating effect on fibroblast migration (31.9%) vs 14.7% with succussed solvent (0712–1) at 1:100 dilutions (p < 0.001). Unsuccussed solvent (0712–3) had no influence on cell migration (6.3%; p > 0.05). Preparation (0712–2) at a dilution of 1:100 promoted <it>in vitro</it> wound closure by 59.5% and differed significantly (p < 0.001) from succussed solvent (0712–1), which caused 22.1% wound closure.</p> <p>Conclusion</p> <p>Results of this study showed that the low potency homeopathic remedy (0712–2) exerted <it>in vitro</it> wound closure potential in NIH 3T3 fibroblasts. This effect resulted from stimulation of fibroblasts motility rather than of their mitosis.</p>http://www.biomedcentral.com/1472-6882/12/100Wound healing3T3 fibroblastsHomeopathic remedy<it>Arnica</it><it>Calendula</it><it>Hypericum</it><it>Symphytum</it> |
| spellingShingle | Hostanska Katarina Rostock Matthias Melzer Joerg Baumgartner Stephan Saller Reinhard A homeopathic remedy from arnica, marigold, St. John’s wort and comfrey accelerates <it>in vitro</it> wound scratch closure of NIH 3T3 fibroblasts BMC Complementary and Alternative Medicine Wound healing 3T3 fibroblasts Homeopathic remedy <it>Arnica</it> <it>Calendula</it> <it>Hypericum</it> <it>Symphytum</it> |
| title | A homeopathic remedy from arnica, marigold, St. John’s wort and comfrey accelerates <it>in vitro</it> wound scratch closure of NIH 3T3 fibroblasts |
| title_full | A homeopathic remedy from arnica, marigold, St. John’s wort and comfrey accelerates <it>in vitro</it> wound scratch closure of NIH 3T3 fibroblasts |
| title_fullStr | A homeopathic remedy from arnica, marigold, St. John’s wort and comfrey accelerates <it>in vitro</it> wound scratch closure of NIH 3T3 fibroblasts |
| title_full_unstemmed | A homeopathic remedy from arnica, marigold, St. John’s wort and comfrey accelerates <it>in vitro</it> wound scratch closure of NIH 3T3 fibroblasts |
| title_short | A homeopathic remedy from arnica, marigold, St. John’s wort and comfrey accelerates <it>in vitro</it> wound scratch closure of NIH 3T3 fibroblasts |
| title_sort | homeopathic remedy from arnica marigold st john s wort and comfrey accelerates it in vitro it wound scratch closure of nih 3t3 fibroblasts |
| topic | Wound healing 3T3 fibroblasts Homeopathic remedy <it>Arnica</it> <it>Calendula</it> <it>Hypericum</it> <it>Symphytum</it> |
| url | http://www.biomedcentral.com/1472-6882/12/100 |
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