Macrophage Phenotype in Combination with Tumor Microbiome Composition Predicts RCC Patients’ Survival: A Pilot Study
The identification of new prognostic markers of renal cell carcinoma (RCC) is an urgent problem in oncourology. To investigate the potential prognostic significance of tumor microbiome and stromal inflammatory markers, we studied a cohort of 66 patients with RCC (23 clear cell RCC, 19 papillary RCC...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/10/7/1516 |
| _version_ | 1797433806014119936 |
|---|---|
| author | Olga V. Kovaleva Polina Podlesnaya Maxim Sorokin Valeria Mochalnikova Vladimir Kataev Yuriy A. Khlopko Andrey O. Plotnikov Ivan S. Stilidi Nikolay E. Kushlinskii Alexei Gratchev |
| author_facet | Olga V. Kovaleva Polina Podlesnaya Maxim Sorokin Valeria Mochalnikova Vladimir Kataev Yuriy A. Khlopko Andrey O. Plotnikov Ivan S. Stilidi Nikolay E. Kushlinskii Alexei Gratchev |
| author_sort | Olga V. Kovaleva |
| collection | DOAJ |
| description | The identification of new prognostic markers of renal cell carcinoma (RCC) is an urgent problem in oncourology. To investigate the potential prognostic significance of tumor microbiome and stromal inflammatory markers, we studied a cohort of 66 patients with RCC (23 clear cell RCC, 19 papillary RCC and 24 chromophobe RCC). The microbiome was analyzed in tumor and normal tissue by 16S rRNA amplicon sequencing. Characterization of the tumor stroma was performed using immunohistochemistry. A significant difference in alpha diversity was demonstrated between normal kidney tissue and all types of RCC. Further, we demonstrated that the bacterial burden was higher in adjacent normal tissue than in a tumor. For the first time, we demonstrated a significant correlation between bacterial burden and the content of PU.1+ macrophages and CD66b+ neutrophils in kidney tumors. Tumors with high content of PU.1+ cells and CD66b+ cells in the stroma were characterized by a lower bacterial burden. In the tumors with high bacterial burden, the number of PU.1+ cells and CD66b+ was associated with a poor prognosis. The identified associations indicate the great prognostic potential of a combined tumor microbiome and stromal cell analysis. |
| first_indexed | 2024-03-09T10:21:58Z |
| format | Article |
| id | doaj.art-1fa75ca5b73e4892b9d04d5a82eb6172 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T10:21:58Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-1fa75ca5b73e4892b9d04d5a82eb61722023-12-01T21:55:39ZengMDPI AGBiomedicines2227-90592022-06-01107151610.3390/biomedicines10071516Macrophage Phenotype in Combination with Tumor Microbiome Composition Predicts RCC Patients’ Survival: A Pilot StudyOlga V. Kovaleva0Polina Podlesnaya1Maxim Sorokin2Valeria Mochalnikova3Vladimir Kataev4Yuriy A. Khlopko5Andrey O. Plotnikov6Ivan S. Stilidi7Nikolay E. Kushlinskii8Alexei Gratchev9N.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, RussiaN.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, RussiaN.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, RussiaN.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, RussiaInstitute for Cellular and Intracellular Symbiosis of the Ural Branch of the Russian Academy of Sciences, 460000 Orenburg, RussiaInstitute for Cellular and Intracellular Symbiosis of the Ural Branch of the Russian Academy of Sciences, 460000 Orenburg, RussiaInstitute for Cellular and Intracellular Symbiosis of the Ural Branch of the Russian Academy of Sciences, 460000 Orenburg, RussiaN.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, RussiaN.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, RussiaN.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, RussiaThe identification of new prognostic markers of renal cell carcinoma (RCC) is an urgent problem in oncourology. To investigate the potential prognostic significance of tumor microbiome and stromal inflammatory markers, we studied a cohort of 66 patients with RCC (23 clear cell RCC, 19 papillary RCC and 24 chromophobe RCC). The microbiome was analyzed in tumor and normal tissue by 16S rRNA amplicon sequencing. Characterization of the tumor stroma was performed using immunohistochemistry. A significant difference in alpha diversity was demonstrated between normal kidney tissue and all types of RCC. Further, we demonstrated that the bacterial burden was higher in adjacent normal tissue than in a tumor. For the first time, we demonstrated a significant correlation between bacterial burden and the content of PU.1+ macrophages and CD66b+ neutrophils in kidney tumors. Tumors with high content of PU.1+ cells and CD66b+ cells in the stroma were characterized by a lower bacterial burden. In the tumors with high bacterial burden, the number of PU.1+ cells and CD66b+ was associated with a poor prognosis. The identified associations indicate the great prognostic potential of a combined tumor microbiome and stromal cell analysis.https://www.mdpi.com/2227-9059/10/7/1516renal cell carcinomaprognosisPU.1microbiomemacrophagestroma |
| spellingShingle | Olga V. Kovaleva Polina Podlesnaya Maxim Sorokin Valeria Mochalnikova Vladimir Kataev Yuriy A. Khlopko Andrey O. Plotnikov Ivan S. Stilidi Nikolay E. Kushlinskii Alexei Gratchev Macrophage Phenotype in Combination with Tumor Microbiome Composition Predicts RCC Patients’ Survival: A Pilot Study Biomedicines renal cell carcinoma prognosis PU.1 microbiome macrophage stroma |
| title | Macrophage Phenotype in Combination with Tumor Microbiome Composition Predicts RCC Patients’ Survival: A Pilot Study |
| title_full | Macrophage Phenotype in Combination with Tumor Microbiome Composition Predicts RCC Patients’ Survival: A Pilot Study |
| title_fullStr | Macrophage Phenotype in Combination with Tumor Microbiome Composition Predicts RCC Patients’ Survival: A Pilot Study |
| title_full_unstemmed | Macrophage Phenotype in Combination with Tumor Microbiome Composition Predicts RCC Patients’ Survival: A Pilot Study |
| title_short | Macrophage Phenotype in Combination with Tumor Microbiome Composition Predicts RCC Patients’ Survival: A Pilot Study |
| title_sort | macrophage phenotype in combination with tumor microbiome composition predicts rcc patients survival a pilot study |
| topic | renal cell carcinoma prognosis PU.1 microbiome macrophage stroma |
| url | https://www.mdpi.com/2227-9059/10/7/1516 |
| work_keys_str_mv | AT olgavkovaleva macrophagephenotypeincombinationwithtumormicrobiomecompositionpredictsrccpatientssurvivalapilotstudy AT polinapodlesnaya macrophagephenotypeincombinationwithtumormicrobiomecompositionpredictsrccpatientssurvivalapilotstudy AT maximsorokin macrophagephenotypeincombinationwithtumormicrobiomecompositionpredictsrccpatientssurvivalapilotstudy AT valeriamochalnikova macrophagephenotypeincombinationwithtumormicrobiomecompositionpredictsrccpatientssurvivalapilotstudy AT vladimirkataev macrophagephenotypeincombinationwithtumormicrobiomecompositionpredictsrccpatientssurvivalapilotstudy AT yuriyakhlopko macrophagephenotypeincombinationwithtumormicrobiomecompositionpredictsrccpatientssurvivalapilotstudy AT andreyoplotnikov macrophagephenotypeincombinationwithtumormicrobiomecompositionpredictsrccpatientssurvivalapilotstudy AT ivansstilidi macrophagephenotypeincombinationwithtumormicrobiomecompositionpredictsrccpatientssurvivalapilotstudy AT nikolayekushlinskii macrophagephenotypeincombinationwithtumormicrobiomecompositionpredictsrccpatientssurvivalapilotstudy AT alexeigratchev macrophagephenotypeincombinationwithtumormicrobiomecompositionpredictsrccpatientssurvivalapilotstudy |