Oral administration of Clostridium butyricum rescues streptomycin-exacerbated respiratory syncytial virus-induced lung inflammation in mice
Changes in the intestinal microbiota indirectly impact the health of mucosa distal to the intestine, particularly the respiratory tract. However, the effects of intestinal microbiota dysbiosis on the regulation of respiratory syncytial virus (RSV) infection are not clear. In this study, we examined...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-12-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21505594.2021.1962137 |
| _version_ | 1798014813339648000 |
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| author | Wenwen Zhu Jia Wang Na Zhao Rui Zheng Dalu Wang Weiwei Liu Beixing Liu |
| author_facet | Wenwen Zhu Jia Wang Na Zhao Rui Zheng Dalu Wang Weiwei Liu Beixing Liu |
| author_sort | Wenwen Zhu |
| collection | DOAJ |
| description | Changes in the intestinal microbiota indirectly impact the health of mucosa distal to the intestine, particularly the respiratory tract. However, the effects of intestinal microbiota dysbiosis on the regulation of respiratory syncytial virus (RSV) infection are not clear. In this study, we examined the effects of altering the intestinal microbiota on the pulmonary immune response against RSV infection. BALB/c mice were treated with streptomycin before infection with RSV to study the altered immune response. The ingestion of streptomycin led to a marked alteration in the intestinal microbiota with a reduced abundance of Lactobacillus and Clostridium genera, followed by greatly aggravated pulmonary inflammation in response to RSV infection. This aggravated inflammation was associated with a dysregulated immune response against RSV infection, characterized by the increased expression of IFN-γ and IL-17 and increased pulmonary M1-like macrophage polarization, and decreased expression of IL-5. Supplementation of Clostridium butyricum (CB) prevented aggravated inflammation and the dysregulated immune response characterized by greater M2 polarization of pulmonary macrophages and decreased release of IFN-γ and IL-17 as well as increased IL-5 levels. Furthermore, in vitro and in vivo experiments identified that butyrate, the main metabolite produced by CB, promoted M2 polarization of macrophages in RSV-infected mice exposed to streptomycin. Together, these results demonstrate the mechanism by which intestinal microbiota modulate the pulmonary immune response to RSV infection. |
| first_indexed | 2024-04-11T15:24:22Z |
| format | Article |
| id | doaj.art-1fa7833f776a4e039c0e3757acb4a73e |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-04-11T15:24:22Z |
| publishDate | 2021-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-1fa7833f776a4e039c0e3757acb4a73e2022-12-22T04:16:18ZengTaylor & Francis GroupVirulence2150-55942150-56082021-12-011212133214810.1080/21505594.2021.19621371962137Oral administration of Clostridium butyricum rescues streptomycin-exacerbated respiratory syncytial virus-induced lung inflammation in miceWenwen Zhu0Jia Wang1Na Zhao2Rui Zheng3Dalu Wang4Weiwei Liu5Beixing Liu6China Medical UniversityChina Medical UniversityChina Medical UniversityChina Medical UniversityChina Medical UniversityChina Medical UniversityChina Medical UniversityChanges in the intestinal microbiota indirectly impact the health of mucosa distal to the intestine, particularly the respiratory tract. However, the effects of intestinal microbiota dysbiosis on the regulation of respiratory syncytial virus (RSV) infection are not clear. In this study, we examined the effects of altering the intestinal microbiota on the pulmonary immune response against RSV infection. BALB/c mice were treated with streptomycin before infection with RSV to study the altered immune response. The ingestion of streptomycin led to a marked alteration in the intestinal microbiota with a reduced abundance of Lactobacillus and Clostridium genera, followed by greatly aggravated pulmonary inflammation in response to RSV infection. This aggravated inflammation was associated with a dysregulated immune response against RSV infection, characterized by the increased expression of IFN-γ and IL-17 and increased pulmonary M1-like macrophage polarization, and decreased expression of IL-5. Supplementation of Clostridium butyricum (CB) prevented aggravated inflammation and the dysregulated immune response characterized by greater M2 polarization of pulmonary macrophages and decreased release of IFN-γ and IL-17 as well as increased IL-5 levels. Furthermore, in vitro and in vivo experiments identified that butyrate, the main metabolite produced by CB, promoted M2 polarization of macrophages in RSV-infected mice exposed to streptomycin. Together, these results demonstrate the mechanism by which intestinal microbiota modulate the pulmonary immune response to RSV infection.http://dx.doi.org/10.1080/21505594.2021.1962137gastrointestinal microbiotarsvimmune responsemacrophagesclostridium butyricumbutyrate |
| spellingShingle | Wenwen Zhu Jia Wang Na Zhao Rui Zheng Dalu Wang Weiwei Liu Beixing Liu Oral administration of Clostridium butyricum rescues streptomycin-exacerbated respiratory syncytial virus-induced lung inflammation in mice Virulence gastrointestinal microbiota rsv immune response macrophages clostridium butyricum butyrate |
| title | Oral administration of Clostridium butyricum rescues streptomycin-exacerbated respiratory syncytial virus-induced lung inflammation in mice |
| title_full | Oral administration of Clostridium butyricum rescues streptomycin-exacerbated respiratory syncytial virus-induced lung inflammation in mice |
| title_fullStr | Oral administration of Clostridium butyricum rescues streptomycin-exacerbated respiratory syncytial virus-induced lung inflammation in mice |
| title_full_unstemmed | Oral administration of Clostridium butyricum rescues streptomycin-exacerbated respiratory syncytial virus-induced lung inflammation in mice |
| title_short | Oral administration of Clostridium butyricum rescues streptomycin-exacerbated respiratory syncytial virus-induced lung inflammation in mice |
| title_sort | oral administration of clostridium butyricum rescues streptomycin exacerbated respiratory syncytial virus induced lung inflammation in mice |
| topic | gastrointestinal microbiota rsv immune response macrophages clostridium butyricum butyrate |
| url | http://dx.doi.org/10.1080/21505594.2021.1962137 |
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