A Study on the Skin Whitening Activity of Digesta from Edible Bird’s Nest: A Mucin Glycoprotein
Edible bird’s nest (EBN) is an unusual mucin glycoprotein. In China, it is popular among consumers due to its skin whitening activity. However, the relationship between protein, sialic acid, and the whitening activity of EBN after digestion is still unclear. In the present work, the whitening activi...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/8/1/24 |
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| author | Qunyan Fan Jianmei Lian Xuncai Liu Fengyang Zou Xin Wang Maoshen Chen |
| author_facet | Qunyan Fan Jianmei Lian Xuncai Liu Fengyang Zou Xin Wang Maoshen Chen |
| author_sort | Qunyan Fan |
| collection | DOAJ |
| description | Edible bird’s nest (EBN) is an unusual mucin glycoprotein. In China, it is popular among consumers due to its skin whitening activity. However, the relationship between protein, sialic acid, and the whitening activity of EBN after digestion is still unclear. In the present work, the whitening activity (antioxidant activity and tyrosinase inhibitory activity) of digested EBN were studied by HepG2 and B16 cell models. The dissolution rate of protein and sialic acid was 49.59% and 46.45% after the simulated digestion, respectively. The contents of free sialic acid and glycan sialic acid in EBN digesta were 17.82% and 12.24%, respectively. HepG2 cell experiment showed that the digested EBN had significant antioxidant activity, with EC<sub>50</sub> of 1.84 mg/mL, and had a protective effect on H<sub>2</sub>O<sub>2</sub>-induced oxidative damage cells. The results of H<sub>2</sub>O<sub>2</sub>-induced oxidative damage showed that the cell survival rate increased from 40% to 57.37% when the concentration of digested EBN was 1 mg/mL. The results of the B16 cell experiment showed that the digested EBN had a significant inhibitory effect on tyrosinase activity, and the EC<sub>50</sub> value of tyrosinase activity was 7.22 mg/mL. Cell experiments showed that free sialic acid had stronger antioxidant activity and tyrosinase inhibitory activity than glycan sialic acid. The contribution rate analysis showed that protein component was the main antioxidant component in digestive products, and the contribution rate was 85.87%; free sialic acid was the main component that inhibited tyrosinase activity, accounting for 63.43%. The products of the complete digestion of EBN are suitable for the development of a new generation of whitening health products. |
| first_indexed | 2024-03-10T01:26:30Z |
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| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-10T01:26:30Z |
| publishDate | 2021-12-01 |
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| record_format | Article |
| series | Gels |
| spelling | doaj.art-1faec784798e4b8aad02eded5aa779bb2023-11-23T13:50:03ZengMDPI AGGels2310-28612021-12-01812410.3390/gels8010024A Study on the Skin Whitening Activity of Digesta from Edible Bird’s Nest: A Mucin GlycoproteinQunyan Fan0Jianmei Lian1Xuncai Liu2Fengyang Zou3Xin Wang4Maoshen Chen5Xiamen Yanzhiwu Sinong Food Co., Ltd., Xiamen 360000, ChinaXiamen Yanzhiwu Sinong Food Co., Ltd., Xiamen 360000, ChinaXiamen Yanzhiwu Sinong Food Co., Ltd., Xiamen 360000, ChinaXiamen Yanzhiwu Sinong Food Co., Ltd., Xiamen 360000, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaEdible bird’s nest (EBN) is an unusual mucin glycoprotein. In China, it is popular among consumers due to its skin whitening activity. However, the relationship between protein, sialic acid, and the whitening activity of EBN after digestion is still unclear. In the present work, the whitening activity (antioxidant activity and tyrosinase inhibitory activity) of digested EBN were studied by HepG2 and B16 cell models. The dissolution rate of protein and sialic acid was 49.59% and 46.45% after the simulated digestion, respectively. The contents of free sialic acid and glycan sialic acid in EBN digesta were 17.82% and 12.24%, respectively. HepG2 cell experiment showed that the digested EBN had significant antioxidant activity, with EC<sub>50</sub> of 1.84 mg/mL, and had a protective effect on H<sub>2</sub>O<sub>2</sub>-induced oxidative damage cells. The results of H<sub>2</sub>O<sub>2</sub>-induced oxidative damage showed that the cell survival rate increased from 40% to 57.37% when the concentration of digested EBN was 1 mg/mL. The results of the B16 cell experiment showed that the digested EBN had a significant inhibitory effect on tyrosinase activity, and the EC<sub>50</sub> value of tyrosinase activity was 7.22 mg/mL. Cell experiments showed that free sialic acid had stronger antioxidant activity and tyrosinase inhibitory activity than glycan sialic acid. The contribution rate analysis showed that protein component was the main antioxidant component in digestive products, and the contribution rate was 85.87%; free sialic acid was the main component that inhibited tyrosinase activity, accounting for 63.43%. The products of the complete digestion of EBN are suitable for the development of a new generation of whitening health products.https://www.mdpi.com/2310-2861/8/1/24edible bird’s nestskin whitening activityantioxidant activitytyrosinase inhibitory activity |
| spellingShingle | Qunyan Fan Jianmei Lian Xuncai Liu Fengyang Zou Xin Wang Maoshen Chen A Study on the Skin Whitening Activity of Digesta from Edible Bird’s Nest: A Mucin Glycoprotein Gels edible bird’s nest skin whitening activity antioxidant activity tyrosinase inhibitory activity |
| title | A Study on the Skin Whitening Activity of Digesta from Edible Bird’s Nest: A Mucin Glycoprotein |
| title_full | A Study on the Skin Whitening Activity of Digesta from Edible Bird’s Nest: A Mucin Glycoprotein |
| title_fullStr | A Study on the Skin Whitening Activity of Digesta from Edible Bird’s Nest: A Mucin Glycoprotein |
| title_full_unstemmed | A Study on the Skin Whitening Activity of Digesta from Edible Bird’s Nest: A Mucin Glycoprotein |
| title_short | A Study on the Skin Whitening Activity of Digesta from Edible Bird’s Nest: A Mucin Glycoprotein |
| title_sort | study on the skin whitening activity of digesta from edible bird s nest a mucin glycoprotein |
| topic | edible bird’s nest skin whitening activity antioxidant activity tyrosinase inhibitory activity |
| url | https://www.mdpi.com/2310-2861/8/1/24 |
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