Possible Role of GADD45γ Methylation in Diffuse Large B-Cell Lymphoma: Does It Affect the Progression and Tissue Involvement?
INTRODUCTION: Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin lymphoma among adults and is characterized by heterogeneous clinical, immunophenotypic, and genetic features. Different mechanisms deregulating cell cycle and apoptosis play a role in the pathogenesis of DLBCL...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2015-11-01
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| Series: | Turkish Journal of Hematology |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-70457 |
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| author | İkbal Cansu Barış Vildan Caner Nilay Şen Türk İsmail Sarı Sibel Hacıoğlu Mehmet Hilmi Doğu Ozan Çetin Emre Tepeli Özge Can Gülseren Bağcı Ali Keskin |
| author_facet | İkbal Cansu Barış Vildan Caner Nilay Şen Türk İsmail Sarı Sibel Hacıoğlu Mehmet Hilmi Doğu Ozan Çetin Emre Tepeli Özge Can Gülseren Bağcı Ali Keskin |
| author_sort | İkbal Cansu Barış |
| collection | DOAJ |
| description | INTRODUCTION: Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin lymphoma among adults and is characterized by heterogeneous clinical, immunophenotypic, and genetic features. Different mechanisms deregulating cell cycle and apoptosis play a role in the pathogenesis of DLBCL. Growth arrest DNA damage-inducible 45 (GADD45γ) is an
important gene family involved in these mechanisms. The aims of this study are to determine the frequency of GADD45γ
methylation, to evaluate the correlation between GADD45γ methylation and protein expression, and to investigate the relation between methylation status and clinicopathologic parameters in DLBCL tissues and reactive lymphoid node tissues from patients with reactive lymphoid hyperplasia. METHODS: Thirty-six tissue samples of DLBCL and 40 nonmalignant reactive lymphoid node tissues were
analyzed in this study. Methylation-sensitive high-resolution melting analysis was used for the determination of GADD45γ methylation status. The GADD45γ protein expression was determined by immunohistochemistry. RESULTS: GADD45γ methylation was frequent (50.0%) in DLBCL. It was also significantly higher in advanced-stage tumors compared with early-stage (p=0.041). In contrast, unmethylated GADD45γ was associated with nodal involvement as the primary anatomical site (p=0.040). DISCUSSION AND CONCLUSION: The results of this study show that, in contrast to solid tumors, the frequency of GADD45γ methylation is higher and this epigenetic alteration of GADD45γ may be associated with progression in DLBCL. In addition, nodal involvement is more likely to be present in patients with unmethylated GADD45γ. |
| first_indexed | 2024-04-10T13:04:34Z |
| format | Article |
| id | doaj.art-1fb16646883546aaa49d8c7ae11fb94a |
| institution | Directory Open Access Journal |
| issn | 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T13:04:34Z |
| publishDate | 2015-11-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-1fb16646883546aaa49d8c7ae11fb94a2023-02-15T16:13:00ZengGalenos Publishing HouseTurkish Journal of Hematology1308-52632015-11-0132429530310.4274/tjh.2014.0174TJH-70457Possible Role of GADD45γ Methylation in Diffuse Large B-Cell Lymphoma: Does It Affect the Progression and Tissue Involvement?İkbal Cansu Barış0Vildan Caner1Nilay Şen Türk2İsmail Sarı3Sibel Hacıoğlu4Mehmet Hilmi Doğu5Ozan Çetin6Emre Tepeli7Özge Can8Gülseren Bağcı9Ali Keskin10Pamukkale University Faculty of Medicine, Department of Medical Biology, Denizli, TurkeyPamukkale University Faculty of Medicine, Department of Medical Biology, Denizli, TurkeyPamukkale University Faculty of Medicine, Department of Medical Pathology, Denizli, TurkeyPamukkale University Faculty of Medicine, Department of Hematology, Denizli, TurkeyPamukkale University Faculty of Medicine, Department of Hematology, Denizli, TurkeyPamukkale University Faculty of Medicine, Department of Hematology, Denizli, TurkeyPamukkale University Faculty of Medicine, Department of Medical Genetics, Denizli, TurkeyPamukkale University Faculty of Medicine, Department of Medical Genetics, Denizli, TurkeyPamukkale University Faculty of Medicine, Department of Medical Biology, Denizli, TurkeyPamukkale University Faculty of Medicine, Department of Medical Biology, Denizli, TurkeyPamukkale University Faculty of Medicine, Department of Hematology, Denizli, TurkeyINTRODUCTION: Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin lymphoma among adults and is characterized by heterogeneous clinical, immunophenotypic, and genetic features. Different mechanisms deregulating cell cycle and apoptosis play a role in the pathogenesis of DLBCL. Growth arrest DNA damage-inducible 45 (GADD45γ) is an important gene family involved in these mechanisms. The aims of this study are to determine the frequency of GADD45γ methylation, to evaluate the correlation between GADD45γ methylation and protein expression, and to investigate the relation between methylation status and clinicopathologic parameters in DLBCL tissues and reactive lymphoid node tissues from patients with reactive lymphoid hyperplasia. METHODS: Thirty-six tissue samples of DLBCL and 40 nonmalignant reactive lymphoid node tissues were analyzed in this study. Methylation-sensitive high-resolution melting analysis was used for the determination of GADD45γ methylation status. The GADD45γ protein expression was determined by immunohistochemistry. RESULTS: GADD45γ methylation was frequent (50.0%) in DLBCL. It was also significantly higher in advanced-stage tumors compared with early-stage (p=0.041). In contrast, unmethylated GADD45γ was associated with nodal involvement as the primary anatomical site (p=0.040). DISCUSSION AND CONCLUSION: The results of this study show that, in contrast to solid tumors, the frequency of GADD45γ methylation is higher and this epigenetic alteration of GADD45γ may be associated with progression in DLBCL. In addition, nodal involvement is more likely to be present in patients with unmethylated GADD45γ.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-70457gadd45γdna methylationdiffuse large b-cell lymphoma |
| spellingShingle | İkbal Cansu Barış Vildan Caner Nilay Şen Türk İsmail Sarı Sibel Hacıoğlu Mehmet Hilmi Doğu Ozan Çetin Emre Tepeli Özge Can Gülseren Bağcı Ali Keskin Possible Role of GADD45γ Methylation in Diffuse Large B-Cell Lymphoma: Does It Affect the Progression and Tissue Involvement? Turkish Journal of Hematology gadd45γ dna methylation diffuse large b-cell lymphoma |
| title | Possible Role of GADD45γ Methylation in Diffuse Large B-Cell Lymphoma: Does It Affect the Progression and Tissue Involvement? |
| title_full | Possible Role of GADD45γ Methylation in Diffuse Large B-Cell Lymphoma: Does It Affect the Progression and Tissue Involvement? |
| title_fullStr | Possible Role of GADD45γ Methylation in Diffuse Large B-Cell Lymphoma: Does It Affect the Progression and Tissue Involvement? |
| title_full_unstemmed | Possible Role of GADD45γ Methylation in Diffuse Large B-Cell Lymphoma: Does It Affect the Progression and Tissue Involvement? |
| title_short | Possible Role of GADD45γ Methylation in Diffuse Large B-Cell Lymphoma: Does It Affect the Progression and Tissue Involvement? |
| title_sort | possible role of gadd45 947 methylation in diffuse large b cell lymphoma does it affect the progression and tissue involvement |
| topic | gadd45γ dna methylation diffuse large b-cell lymphoma |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-70457 |
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