G-Quadruplex-Forming Aptamers—Characteristics, Applications, and Perspectives
G-quadruplexes constitute a unique class of nucleic acid structures formed by G-rich oligonucleotides of DNA- or RNA-type. Depending on their chemical nature, loops length, and localization in the sequence or structure molecularity, G-quadruplexes are highly polymorphic structures showing various fo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/20/3781 |
| _version_ | 1818953974640803840 |
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| author | Carolina Roxo Weronika Kotkowiak Anna Pasternak |
| author_facet | Carolina Roxo Weronika Kotkowiak Anna Pasternak |
| author_sort | Carolina Roxo |
| collection | DOAJ |
| description | G-quadruplexes constitute a unique class of nucleic acid structures formed by G-rich oligonucleotides of DNA- or RNA-type. Depending on their chemical nature, loops length, and localization in the sequence or structure molecularity, G-quadruplexes are highly polymorphic structures showing various folding topologies. They may be formed in the human genome where they are believed to play a pivotal role in the regulation of multiple biological processes such as replication, transcription, and translation. Thus, natural G-quadruplex structures became prospective targets for disease treatment. The fast development of systematic evolution of ligands by exponential enrichment (SELEX) technologies provided a number of G-rich aptamers revealing the potential of G-quadruplex structures as a promising molecular tool targeted toward various biologically important ligands. Because of their high stability, increased cellular uptake, ease of chemical modification, minor production costs, and convenient storage, G-rich aptamers became interesting therapeutic and diagnostic alternatives to antibodies. In this review, we describe the recent advances in the development of G-quadruplex based aptamers by focusing on the therapeutic and diagnostic potential of this exceptional class of nucleic acid structures. |
| first_indexed | 2024-12-20T10:14:48Z |
| format | Article |
| id | doaj.art-1fb221ffea76444ab3b492c6030e7292 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-20T10:14:48Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1fb221ffea76444ab3b492c6030e72922022-12-21T19:44:05ZengMDPI AGMolecules1420-30492019-10-012420378110.3390/molecules24203781molecules24203781G-Quadruplex-Forming Aptamers—Characteristics, Applications, and PerspectivesCarolina Roxo0Weronika Kotkowiak1Anna Pasternak2Department of Nucleic Acids Bioengineering, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, PolandDepartment of Nucleic Acids Bioengineering, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, PolandDepartment of Nucleic Acids Bioengineering, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, PolandG-quadruplexes constitute a unique class of nucleic acid structures formed by G-rich oligonucleotides of DNA- or RNA-type. Depending on their chemical nature, loops length, and localization in the sequence or structure molecularity, G-quadruplexes are highly polymorphic structures showing various folding topologies. They may be formed in the human genome where they are believed to play a pivotal role in the regulation of multiple biological processes such as replication, transcription, and translation. Thus, natural G-quadruplex structures became prospective targets for disease treatment. The fast development of systematic evolution of ligands by exponential enrichment (SELEX) technologies provided a number of G-rich aptamers revealing the potential of G-quadruplex structures as a promising molecular tool targeted toward various biologically important ligands. Because of their high stability, increased cellular uptake, ease of chemical modification, minor production costs, and convenient storage, G-rich aptamers became interesting therapeutic and diagnostic alternatives to antibodies. In this review, we describe the recent advances in the development of G-quadruplex based aptamers by focusing on the therapeutic and diagnostic potential of this exceptional class of nucleic acid structures.https://www.mdpi.com/1420-3049/24/20/3781aptamersg-quadruplexescanceranticoagulantsaptasensorsantiviral agentstherapeuticsdiagnosticsconjugates |
| spellingShingle | Carolina Roxo Weronika Kotkowiak Anna Pasternak G-Quadruplex-Forming Aptamers—Characteristics, Applications, and Perspectives Molecules aptamers g-quadruplexes cancer anticoagulants aptasensors antiviral agents therapeutics diagnostics conjugates |
| title | G-Quadruplex-Forming Aptamers—Characteristics, Applications, and Perspectives |
| title_full | G-Quadruplex-Forming Aptamers—Characteristics, Applications, and Perspectives |
| title_fullStr | G-Quadruplex-Forming Aptamers—Characteristics, Applications, and Perspectives |
| title_full_unstemmed | G-Quadruplex-Forming Aptamers—Characteristics, Applications, and Perspectives |
| title_short | G-Quadruplex-Forming Aptamers—Characteristics, Applications, and Perspectives |
| title_sort | g quadruplex forming aptamers characteristics applications and perspectives |
| topic | aptamers g-quadruplexes cancer anticoagulants aptasensors antiviral agents therapeutics diagnostics conjugates |
| url | https://www.mdpi.com/1420-3049/24/20/3781 |
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