Karyotypic description of the stingless bee Melipona quinquefasciata Lepeletier, 1836 (Hymenoptera, Meliponini) with emphasis on the presence of B chromosomes
Stingless bees are distributed widely in the tropics, where they are major pollinators of several plant species. In this study, the karyotype of Melipona quinquefasciata Lepeletier, 1836 was analysed, with emphasis on the presence of B chromosomes. Post-defecating larvae were analysed using Giemsa s...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2018-11-01
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| Series: | Comparative Cytogenetics |
| Online Access: | https://compcytogen.pensoft.net/articles.php?id=29165 |
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| author | Alexandra Avelar Silva Marla Piumbini Rocha Silvia das Graças Pompolo Lucio Antonio de Oliveira Campos Mara Garcia Tavares |
| author_facet | Alexandra Avelar Silva Marla Piumbini Rocha Silvia das Graças Pompolo Lucio Antonio de Oliveira Campos Mara Garcia Tavares |
| author_sort | Alexandra Avelar Silva |
| collection | DOAJ |
| description | Stingless bees are distributed widely in the tropics, where they are major pollinators of several plant species. In this study, the karyotype of Melipona quinquefasciata Lepeletier, 1836 was analysed, with emphasis on the presence of B chromosomes. Post-defecating larvae were analysed using Giemsa staining, the C-banding technique, sequential staining with fluorochromes, and FISH. The chromosome number ranged from 2n = 18 to 22 (females) and from n = 9 to 13 (males) due to the presence of 0–4 B chromosomes. This result demonstrates that M. quinquefasciata has the same chromosomal number as other Melipona Illiger, 1806 species. Considering the A complement, heterochromatin was located only in the pericentromeric region of pair 1. Staining with chromomycin A3 (CMA3) and labelling with rDNA probe, indicated that this region corresponded to the nucleolus organising region. The B chromosomes of M. quinquefasciata could be found in individuals from different localities, they were completely heterochromatic (C-banding) and uniformly stained by 4’,6-diamidino-2-phenylindole (DAPI). Variations in the number of B chromosomes were detected between cells of the same individual, between individuals of the same colony, and between colonies from different localities. |
| first_indexed | 2024-12-12T05:24:01Z |
| format | Article |
| id | doaj.art-1fba43d17208499da1d947fffdd0306a |
| institution | Directory Open Access Journal |
| issn | 1993-0771 1993-078X |
| language | English |
| last_indexed | 2024-12-12T05:24:01Z |
| publishDate | 2018-11-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Comparative Cytogenetics |
| spelling | doaj.art-1fba43d17208499da1d947fffdd0306a2022-12-22T00:36:32ZengPensoft PublishersComparative Cytogenetics1993-07711993-078X2018-11-0112447148210.3897/CompCytogen.v12i4.2916529165Karyotypic description of the stingless bee Melipona quinquefasciata Lepeletier, 1836 (Hymenoptera, Meliponini) with emphasis on the presence of B chromosomesAlexandra Avelar Silva0Marla Piumbini Rocha1Silvia das Graças Pompolo2Lucio Antonio de Oliveira Campos3Mara Garcia Tavares4Universidade Federal de ViçosaUniversidade Federal de PelotasUniversidade Federal de ViçosaUniversidade Federal de ViçosaUniversidade Federal de ViçosaStingless bees are distributed widely in the tropics, where they are major pollinators of several plant species. In this study, the karyotype of Melipona quinquefasciata Lepeletier, 1836 was analysed, with emphasis on the presence of B chromosomes. Post-defecating larvae were analysed using Giemsa staining, the C-banding technique, sequential staining with fluorochromes, and FISH. The chromosome number ranged from 2n = 18 to 22 (females) and from n = 9 to 13 (males) due to the presence of 0–4 B chromosomes. This result demonstrates that M. quinquefasciata has the same chromosomal number as other Melipona Illiger, 1806 species. Considering the A complement, heterochromatin was located only in the pericentromeric region of pair 1. Staining with chromomycin A3 (CMA3) and labelling with rDNA probe, indicated that this region corresponded to the nucleolus organising region. The B chromosomes of M. quinquefasciata could be found in individuals from different localities, they were completely heterochromatic (C-banding) and uniformly stained by 4’,6-diamidino-2-phenylindole (DAPI). Variations in the number of B chromosomes were detected between cells of the same individual, between individuals of the same colony, and between colonies from different localities.https://compcytogen.pensoft.net/articles.php?id=29165 |
| spellingShingle | Alexandra Avelar Silva Marla Piumbini Rocha Silvia das Graças Pompolo Lucio Antonio de Oliveira Campos Mara Garcia Tavares Karyotypic description of the stingless bee Melipona quinquefasciata Lepeletier, 1836 (Hymenoptera, Meliponini) with emphasis on the presence of B chromosomes Comparative Cytogenetics |
| title | Karyotypic description of the stingless bee Melipona quinquefasciata Lepeletier, 1836 (Hymenoptera, Meliponini) with emphasis on the presence of B chromosomes |
| title_full | Karyotypic description of the stingless bee Melipona quinquefasciata Lepeletier, 1836 (Hymenoptera, Meliponini) with emphasis on the presence of B chromosomes |
| title_fullStr | Karyotypic description of the stingless bee Melipona quinquefasciata Lepeletier, 1836 (Hymenoptera, Meliponini) with emphasis on the presence of B chromosomes |
| title_full_unstemmed | Karyotypic description of the stingless bee Melipona quinquefasciata Lepeletier, 1836 (Hymenoptera, Meliponini) with emphasis on the presence of B chromosomes |
| title_short | Karyotypic description of the stingless bee Melipona quinquefasciata Lepeletier, 1836 (Hymenoptera, Meliponini) with emphasis on the presence of B chromosomes |
| title_sort | karyotypic description of the stingless bee melipona quinquefasciata lepeletier 1836 hymenoptera meliponini with emphasis on the presence of b chromosomes |
| url | https://compcytogen.pensoft.net/articles.php?id=29165 |
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