Nanoparticle-Doped Hybrid Polyelectrolyte Microcapsules with Controlled Photoluminescence for Potential Bioimaging Applications
Fluorescent imaging is widely used in the diagnosis and tracking of the distribution, interaction, and transformation processes at molecular, cellular, and tissue levels. To be detectable, delivery systems should exhibit a strong and bright fluorescence. Quantum dots (QDs) are highly photostable flu...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Polymers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4360/13/23/4076 |
| _version_ | 1797507334954549248 |
|---|---|
| author | Galina Nifontova Victor Krivenkov Mariya Zvaigzne Anton Efimov Evgeny Korostylev Sergei Zarubin Alexander Karaulov Igor Nabiev Alyona Sukhanova |
| author_facet | Galina Nifontova Victor Krivenkov Mariya Zvaigzne Anton Efimov Evgeny Korostylev Sergei Zarubin Alexander Karaulov Igor Nabiev Alyona Sukhanova |
| author_sort | Galina Nifontova |
| collection | DOAJ |
| description | Fluorescent imaging is widely used in the diagnosis and tracking of the distribution, interaction, and transformation processes at molecular, cellular, and tissue levels. To be detectable, delivery systems should exhibit a strong and bright fluorescence. Quantum dots (QDs) are highly photostable fluorescent semiconductor nanocrystals with wide absorption spectra and narrow, size-tunable emission spectra, which make them suitable fluorescent nanolabels to be embedded into microparticles used as bioimaging and theranostic agents. The layer-by-layer deposition approach allows the entrapping of QDs, resulting in bright fluorescent microcapsules with tunable surface charge, size, rigidity, and functional properties. Here, we report on the engineering and validation of the structural and photoluminescent characteristics of nanoparticle-doped hybrid microcapsules assembled by the deposition of alternating oppositely charged polyelectrolytes, water-soluble PEGylated core/shell QDs with a cadmium selenide core and a zinc sulfide shell (CdSe/ZnS), and carboxylated magnetic nanoparticles (MNPs) onto calcium carbonate microtemplates. The results demonstrate the efficiency of the layer-by-layer approach to designing QD-, MNP-doped microcapsules with controlled photoluminescence properties, and pave the way for the further development of next-generation bioimaging agents based on hybrid materials for continuous fluorescence imaging. |
| first_indexed | 2024-03-10T04:47:05Z |
| format | Article |
| id | doaj.art-1fbe3f4cd40648b38b49980e8c770e88 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T04:47:05Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-1fbe3f4cd40648b38b49980e8c770e882023-11-23T02:52:31ZengMDPI AGPolymers2073-43602021-11-011323407610.3390/polym13234076Nanoparticle-Doped Hybrid Polyelectrolyte Microcapsules with Controlled Photoluminescence for Potential Bioimaging ApplicationsGalina Nifontova0Victor Krivenkov1Mariya Zvaigzne2Anton Efimov3Evgeny Korostylev4Sergei Zarubin5Alexander Karaulov6Igor Nabiev7Alyona Sukhanova8Laboratoire de Recherche en Nanosciences, LRN-EA4682, Université de Reims Champagne-Ardenne, 51100 Reims, FranceLaboratory of Nano-Bioengineering, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 115409 Moscow, RussiaLaboratory of Nano-Bioengineering, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 115409 Moscow, RussiaShumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaMoscow Institute of Physics and Technology (State University), Dolgoprudny, 141701 Moscow, RussiaMoscow Institute of Physics and Technology (State University), Dolgoprudny, 141701 Moscow, RussiaDepartment of Clinical Immunology and Allergology, Institute of Molecular Medicine, Sechenov First Moscow State Medical University (Sechenov University), 119146 Moscow, RussiaLaboratoire de Recherche en Nanosciences, LRN-EA4682, Université de Reims Champagne-Ardenne, 51100 Reims, FranceLaboratoire de Recherche en Nanosciences, LRN-EA4682, Université de Reims Champagne-Ardenne, 51100 Reims, FranceFluorescent imaging is widely used in the diagnosis and tracking of the distribution, interaction, and transformation processes at molecular, cellular, and tissue levels. To be detectable, delivery systems should exhibit a strong and bright fluorescence. Quantum dots (QDs) are highly photostable fluorescent semiconductor nanocrystals with wide absorption spectra and narrow, size-tunable emission spectra, which make them suitable fluorescent nanolabels to be embedded into microparticles used as bioimaging and theranostic agents. The layer-by-layer deposition approach allows the entrapping of QDs, resulting in bright fluorescent microcapsules with tunable surface charge, size, rigidity, and functional properties. Here, we report on the engineering and validation of the structural and photoluminescent characteristics of nanoparticle-doped hybrid microcapsules assembled by the deposition of alternating oppositely charged polyelectrolytes, water-soluble PEGylated core/shell QDs with a cadmium selenide core and a zinc sulfide shell (CdSe/ZnS), and carboxylated magnetic nanoparticles (MNPs) onto calcium carbonate microtemplates. The results demonstrate the efficiency of the layer-by-layer approach to designing QD-, MNP-doped microcapsules with controlled photoluminescence properties, and pave the way for the further development of next-generation bioimaging agents based on hybrid materials for continuous fluorescence imaging.https://www.mdpi.com/2073-4360/13/23/4076quantum dotsmagnetic nanoparticleshybrid polyelectrolyte microcapsulesnanoparticle-encodingphotobrighteningultramicrostructure |
| spellingShingle | Galina Nifontova Victor Krivenkov Mariya Zvaigzne Anton Efimov Evgeny Korostylev Sergei Zarubin Alexander Karaulov Igor Nabiev Alyona Sukhanova Nanoparticle-Doped Hybrid Polyelectrolyte Microcapsules with Controlled Photoluminescence for Potential Bioimaging Applications Polymers quantum dots magnetic nanoparticles hybrid polyelectrolyte microcapsules nanoparticle-encoding photobrightening ultramicrostructure |
| title | Nanoparticle-Doped Hybrid Polyelectrolyte Microcapsules with Controlled Photoluminescence for Potential Bioimaging Applications |
| title_full | Nanoparticle-Doped Hybrid Polyelectrolyte Microcapsules with Controlled Photoluminescence for Potential Bioimaging Applications |
| title_fullStr | Nanoparticle-Doped Hybrid Polyelectrolyte Microcapsules with Controlled Photoluminescence for Potential Bioimaging Applications |
| title_full_unstemmed | Nanoparticle-Doped Hybrid Polyelectrolyte Microcapsules with Controlled Photoluminescence for Potential Bioimaging Applications |
| title_short | Nanoparticle-Doped Hybrid Polyelectrolyte Microcapsules with Controlled Photoluminescence for Potential Bioimaging Applications |
| title_sort | nanoparticle doped hybrid polyelectrolyte microcapsules with controlled photoluminescence for potential bioimaging applications |
| topic | quantum dots magnetic nanoparticles hybrid polyelectrolyte microcapsules nanoparticle-encoding photobrightening ultramicrostructure |
| url | https://www.mdpi.com/2073-4360/13/23/4076 |
| work_keys_str_mv | AT galinanifontova nanoparticledopedhybridpolyelectrolytemicrocapsuleswithcontrolledphotoluminescenceforpotentialbioimagingapplications AT victorkrivenkov nanoparticledopedhybridpolyelectrolytemicrocapsuleswithcontrolledphotoluminescenceforpotentialbioimagingapplications AT mariyazvaigzne nanoparticledopedhybridpolyelectrolytemicrocapsuleswithcontrolledphotoluminescenceforpotentialbioimagingapplications AT antonefimov nanoparticledopedhybridpolyelectrolytemicrocapsuleswithcontrolledphotoluminescenceforpotentialbioimagingapplications AT evgenykorostylev nanoparticledopedhybridpolyelectrolytemicrocapsuleswithcontrolledphotoluminescenceforpotentialbioimagingapplications AT sergeizarubin nanoparticledopedhybridpolyelectrolytemicrocapsuleswithcontrolledphotoluminescenceforpotentialbioimagingapplications AT alexanderkaraulov nanoparticledopedhybridpolyelectrolytemicrocapsuleswithcontrolledphotoluminescenceforpotentialbioimagingapplications AT igornabiev nanoparticledopedhybridpolyelectrolytemicrocapsuleswithcontrolledphotoluminescenceforpotentialbioimagingapplications AT alyonasukhanova nanoparticledopedhybridpolyelectrolytemicrocapsuleswithcontrolledphotoluminescenceforpotentialbioimagingapplications |