Exploring the Proteomic Profile of Soybean Bran: Unlocking the Potential for Improving Protein Quality and Quantity
Soybean is a rich source of vegetal protein for both animal and human consumption. Despite the high levels of protein in soybean seeds, industrial processing to obtain soybean bran significantly decreases the final protein content of the byproducts. To overcome this problem, cultivars with higher pr...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/14/2704 |
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| author | Mayla Daiane Corre Molinari Renata Fuganti-Pagliarini Yanbao Yu Lilian Hasegawa Florentino Liliane Marcia Mertz-Henning Rayane Nunes Lima Daniela Matias de Carvalho Bittencourt Marcelo Oliveira Freire Elibio Rech |
| author_facet | Mayla Daiane Corre Molinari Renata Fuganti-Pagliarini Yanbao Yu Lilian Hasegawa Florentino Liliane Marcia Mertz-Henning Rayane Nunes Lima Daniela Matias de Carvalho Bittencourt Marcelo Oliveira Freire Elibio Rech |
| author_sort | Mayla Daiane Corre Molinari |
| collection | DOAJ |
| description | Soybean is a rich source of vegetal protein for both animal and human consumption. Despite the high levels of protein in soybean seeds, industrial processing to obtain soybean bran significantly decreases the final protein content of the byproducts. To overcome this problem, cultivars with higher protein contents must be developed. However, selecting the target proteins is difficult because of the lack of information on the proteome profile of soybean bran. Therefore, this study obtained the comparative proteomic profiles of both natural coatless seeds and defatted bran from an elite tropical-soybean cultivar. Thus, their extracts were characterized using LC–MS/MS and a total of 550 proteins were identified. Among these, 526 proteins were detected in coatless seeds and 319 proteins in defatted bran. Moreover, a total of 139 proteins were identified as presenting different levels of content in coatless seeds and defatted bran. Among them, only 46 were retained after the seed processing. These proteins were clustered in several important metabolic pathways, such as amino-acid biosynthesis, sugar biosynthesis, and antioxidant activity, meaning that they could act as targets for bioactive products or genome editing to improve protein quality and quantity in soybean grains. These findings can enhance our understanding regarding protein robustness for both soybean crops and the commercial bran improvement because target proteins must remain intact after processing and must be bioactive when overexpressed. Overall, the soybean bran proteomic profile was explored for the first time, providing a valuable catalogue of target proteins that can tolerate the industrial process. |
| first_indexed | 2024-03-11T00:42:43Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T00:42:43Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-1fbeab467f734a14b9c265ef454eb8a22023-11-18T21:00:52ZengMDPI AGPlants2223-77472023-07-011214270410.3390/plants12142704Exploring the Proteomic Profile of Soybean Bran: Unlocking the Potential for Improving Protein Quality and QuantityMayla Daiane Corre Molinari0Renata Fuganti-Pagliarini1Yanbao Yu2Lilian Hasegawa Florentino3Liliane Marcia Mertz-Henning4Rayane Nunes Lima5Daniela Matias de Carvalho Bittencourt6Marcelo Oliveira Freire7Elibio Rech8Arthur Bernardes Foundation, Embrapa Soybean, Londrina 86085-981, BrazilPiccola Scientific Consulting, Saskatoon, SK S7P 0B6, CanadaJ. Craig Venter Institute, Rockville, MD 20850, USAEmbrapa Genetic Resources and Biotechnology, National Institute of Science and Technology in Synthetic Biology, Distrito Federal 70770-917, BrazilEmbrapa Soybean, Londrina 86085-981, BrazilEmbrapa Genetic Resources and Biotechnology, National Institute of Science and Technology in Synthetic Biology, Distrito Federal 70770-917, BrazilEmbrapa Genetic Resources and Biotechnology, National Institute of Science and Technology in Synthetic Biology, Distrito Federal 70770-917, BrazilJ. Craig Venter Institute, La Jolla, CA 92037, USAEmbrapa Genetic Resources and Biotechnology, National Institute of Science and Technology in Synthetic Biology, Distrito Federal 70770-917, BrazilSoybean is a rich source of vegetal protein for both animal and human consumption. Despite the high levels of protein in soybean seeds, industrial processing to obtain soybean bran significantly decreases the final protein content of the byproducts. To overcome this problem, cultivars with higher protein contents must be developed. However, selecting the target proteins is difficult because of the lack of information on the proteome profile of soybean bran. Therefore, this study obtained the comparative proteomic profiles of both natural coatless seeds and defatted bran from an elite tropical-soybean cultivar. Thus, their extracts were characterized using LC–MS/MS and a total of 550 proteins were identified. Among these, 526 proteins were detected in coatless seeds and 319 proteins in defatted bran. Moreover, a total of 139 proteins were identified as presenting different levels of content in coatless seeds and defatted bran. Among them, only 46 were retained after the seed processing. These proteins were clustered in several important metabolic pathways, such as amino-acid biosynthesis, sugar biosynthesis, and antioxidant activity, meaning that they could act as targets for bioactive products or genome editing to improve protein quality and quantity in soybean grains. These findings can enhance our understanding regarding protein robustness for both soybean crops and the commercial bran improvement because target proteins must remain intact after processing and must be bioactive when overexpressed. Overall, the soybean bran proteomic profile was explored for the first time, providing a valuable catalogue of target proteins that can tolerate the industrial process.https://www.mdpi.com/2223-7747/12/14/2704<i>Glycine max</i>BRS 537industrial processingbioactive proteinsgrain qualityLC–MS/MS |
| spellingShingle | Mayla Daiane Corre Molinari Renata Fuganti-Pagliarini Yanbao Yu Lilian Hasegawa Florentino Liliane Marcia Mertz-Henning Rayane Nunes Lima Daniela Matias de Carvalho Bittencourt Marcelo Oliveira Freire Elibio Rech Exploring the Proteomic Profile of Soybean Bran: Unlocking the Potential for Improving Protein Quality and Quantity Plants <i>Glycine max</i> BRS 537 industrial processing bioactive proteins grain quality LC–MS/MS |
| title | Exploring the Proteomic Profile of Soybean Bran: Unlocking the Potential for Improving Protein Quality and Quantity |
| title_full | Exploring the Proteomic Profile of Soybean Bran: Unlocking the Potential for Improving Protein Quality and Quantity |
| title_fullStr | Exploring the Proteomic Profile of Soybean Bran: Unlocking the Potential for Improving Protein Quality and Quantity |
| title_full_unstemmed | Exploring the Proteomic Profile of Soybean Bran: Unlocking the Potential for Improving Protein Quality and Quantity |
| title_short | Exploring the Proteomic Profile of Soybean Bran: Unlocking the Potential for Improving Protein Quality and Quantity |
| title_sort | exploring the proteomic profile of soybean bran unlocking the potential for improving protein quality and quantity |
| topic | <i>Glycine max</i> BRS 537 industrial processing bioactive proteins grain quality LC–MS/MS |
| url | https://www.mdpi.com/2223-7747/12/14/2704 |
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