Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells
Invasion of periodontal pathogens into periodontal tissues is an important step that can cause tissue destruction in periodontal diseases. Porphyromonas gingivalis is a keystone pathogen and its gingipains are key virulence factors. Fusobacterium nucleatum is a bridge organism that mediates coadhesi...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20002297.2017.1320193 |
| _version_ | 1819182356904280064 |
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| author | Young-Jung Jung Hye-Kyoung Jun Bong-Kyu Choi |
| author_facet | Young-Jung Jung Hye-Kyoung Jun Bong-Kyu Choi |
| author_sort | Young-Jung Jung |
| collection | DOAJ |
| description | Invasion of periodontal pathogens into periodontal tissues is an important step that can cause tissue destruction in periodontal diseases. Porphyromonas gingivalis is a keystone pathogen and its gingipains are key virulence factors. Fusobacterium nucleatum is a bridge organism that mediates coadhesion of disease-causing late colonizers such as P. gingivalis and early colonizers during the development of dental biofilms. The aim of this study was to investigate how P. gingivalis, in particular its gingipains, influences the invasion of coinfecting F. nucleatum into gingival epithelial cells. When invasion of F. nucleatum was analyzed after 4 h of infection, invasion of F. nucleatum was suppressed in the presence of P. gingivalis compared with during monoinfection. However, coinfection with a gingipain-null mutant of P. gingivalis did not affect invasion of F. nucleatum. Inhibition of PI3K reduced invasion of F. nucleatum. P. gingivalis inactivated the PI3K/AKT pathway, which was also dependent on gingipains. Survival of intracellular F. nucleatum was promoted by P. gingivalis with Arg gingipain mutation. The results suggest that P. gingivalis, in particular its gingipains, can affect the invasion of coinfecting F. nucleatum through modulating intracellular signaling of the host cells. |
| first_indexed | 2024-12-22T22:44:50Z |
| format | Article |
| id | doaj.art-1fbf1b1fae0e4b92a1d1111c41564c69 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-22T22:44:50Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-1fbf1b1fae0e4b92a1d1111c41564c692022-12-21T18:10:06ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972017-01-019110.1080/20002297.2017.13201931320193Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cellsYoung-Jung Jung0Hye-Kyoung Jun1Bong-Kyu Choi2School of Dentistry, University of LouisvilleSchool of Dentistry, University of LouisvilleSchool of Dentistry, University of LouisvilleInvasion of periodontal pathogens into periodontal tissues is an important step that can cause tissue destruction in periodontal diseases. Porphyromonas gingivalis is a keystone pathogen and its gingipains are key virulence factors. Fusobacterium nucleatum is a bridge organism that mediates coadhesion of disease-causing late colonizers such as P. gingivalis and early colonizers during the development of dental biofilms. The aim of this study was to investigate how P. gingivalis, in particular its gingipains, influences the invasion of coinfecting F. nucleatum into gingival epithelial cells. When invasion of F. nucleatum was analyzed after 4 h of infection, invasion of F. nucleatum was suppressed in the presence of P. gingivalis compared with during monoinfection. However, coinfection with a gingipain-null mutant of P. gingivalis did not affect invasion of F. nucleatum. Inhibition of PI3K reduced invasion of F. nucleatum. P. gingivalis inactivated the PI3K/AKT pathway, which was also dependent on gingipains. Survival of intracellular F. nucleatum was promoted by P. gingivalis with Arg gingipain mutation. The results suggest that P. gingivalis, in particular its gingipains, can affect the invasion of coinfecting F. nucleatum through modulating intracellular signaling of the host cells.http://dx.doi.org/10.1080/20002297.2017.1320193Gingipainmixed infectionperiodontopathogensgingival epithelial cellsinvasionPI3K/AKT pathway |
| spellingShingle | Young-Jung Jung Hye-Kyoung Jun Bong-Kyu Choi Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells Journal of Oral Microbiology Gingipain mixed infection periodontopathogens gingival epithelial cells invasion PI3K/AKT pathway |
| title | Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells |
| title_full | Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells |
| title_fullStr | Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells |
| title_full_unstemmed | Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells |
| title_short | Porphyromonas gingivalis suppresses invasion of Fusobacterium nucleatum into gingival epithelial cells |
| title_sort | porphyromonas gingivalis suppresses invasion of fusobacterium nucleatum into gingival epithelial cells |
| topic | Gingipain mixed infection periodontopathogens gingival epithelial cells invasion PI3K/AKT pathway |
| url | http://dx.doi.org/10.1080/20002297.2017.1320193 |
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