L-selectin gene polymorphism and its association with clinical mastitis, somatic cell score, and milk production in Polish Holstein-Friesian cattle
Bovine mastitis is a widespread disease of the mammary gland, highly contributing to the increase in veterinary costs in dairy industry. In the present study, the genetic polymorphism within bovine L-selectin gene was analysed and its impact on clinical mastitis occurrence, somatic cell score (SCS),...
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| Format: | Article |
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Czech Academy of Agricultural Sciences
2018-07-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201807-0002_l-selectin-gene-polymorphism-and-its-association-with-clinical-mastitis-somatic-cell-score-and-milk-productio.php |
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| author | Magdalena Dusza Joanna Pokorska Joanna Makulska Dominika Kulaj Michal Cupial |
| author_facet | Magdalena Dusza Joanna Pokorska Joanna Makulska Dominika Kulaj Michal Cupial |
| author_sort | Magdalena Dusza |
| collection | DOAJ |
| description | Bovine mastitis is a widespread disease of the mammary gland, highly contributing to the increase in veterinary costs in dairy industry. In the present study, the genetic polymorphism within bovine L-selectin gene was analysed and its impact on clinical mastitis occurrence, somatic cell score (SCS), and milk production traits in Polish Holstein-Friesian cows was examined. Polymorphism within L-selectin gene, molecule responsible for neutrophil attachment to endothelium, might have a potential role in immune response to bacterial infections and udder health. Two hundred and six Polish Holstein-Friesian cows were genotyped by polymerase chain reaction-restriction fragment length polymorphism method. Two single nucleotide polymorphisms mutations within the coding sequence of L-selectin gene were identified (c.165G>A and c.567C>T). The effect of c.165G>A and c.567C>T mutations on SCS was highly significant (P = 0.0019 and P = 0.0003, respectively). Strong associations (P ≤ 0.0001) were also observed between L-selectin polymorphism and milk production traits (milk yield, milk fat percentage, and milk protein percentage). However, the polymorphism in the analysed gene had no influence on the resistance or susceptibility of cows to clinical mastitis (only the tendency toward significance, P = 0.06 for c.567C>T mutation was found). Potential exploitation of the information on the identified associations in genetic selection needs to confirm the obtained results in further investigations. |
| first_indexed | 2024-04-10T08:24:44Z |
| format | Article |
| id | doaj.art-1fc1899a20024fc58dee4c702cb4c8df |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:24:44Z |
| publishDate | 2018-07-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-1fc1899a20024fc58dee4c702cb4c8df2023-02-23T03:33:35ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092018-07-0163725626210.17221/96/2017-CJAScjs-201807-0002L-selectin gene polymorphism and its association with clinical mastitis, somatic cell score, and milk production in Polish Holstein-Friesian cattleMagdalena Dusza0Joanna Pokorska1Joanna Makulska2Dominika Kulaj3Michal Cupial4Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Krakow, Krakow, PolandDepartment of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Krakow, Krakow, PolandDepartment of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Krakow, Krakow, PolandDepartment of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Krakow, Krakow, PolandInstitute of Agricultural Engineering and Informatics, University of Agriculture in Krakow, Krakow, PolandBovine mastitis is a widespread disease of the mammary gland, highly contributing to the increase in veterinary costs in dairy industry. In the present study, the genetic polymorphism within bovine L-selectin gene was analysed and its impact on clinical mastitis occurrence, somatic cell score (SCS), and milk production traits in Polish Holstein-Friesian cows was examined. Polymorphism within L-selectin gene, molecule responsible for neutrophil attachment to endothelium, might have a potential role in immune response to bacterial infections and udder health. Two hundred and six Polish Holstein-Friesian cows were genotyped by polymerase chain reaction-restriction fragment length polymorphism method. Two single nucleotide polymorphisms mutations within the coding sequence of L-selectin gene were identified (c.165G>A and c.567C>T). The effect of c.165G>A and c.567C>T mutations on SCS was highly significant (P = 0.0019 and P = 0.0003, respectively). Strong associations (P ≤ 0.0001) were also observed between L-selectin polymorphism and milk production traits (milk yield, milk fat percentage, and milk protein percentage). However, the polymorphism in the analysed gene had no influence on the resistance or susceptibility of cows to clinical mastitis (only the tendency toward significance, P = 0.06 for c.567C>T mutation was found). Potential exploitation of the information on the identified associations in genetic selection needs to confirm the obtained results in further investigations.https://cjas.agriculturejournals.cz/artkey/cjs-201807-0002_l-selectin-gene-polymorphism-and-its-association-with-clinical-mastitis-somatic-cell-score-and-milk-productio.phpselludderinflammationmilk production traitscows |
| spellingShingle | Magdalena Dusza Joanna Pokorska Joanna Makulska Dominika Kulaj Michal Cupial L-selectin gene polymorphism and its association with clinical mastitis, somatic cell score, and milk production in Polish Holstein-Friesian cattle Czech Journal of Animal Science sell udder inflammation milk production traits cows |
| title | L-selectin gene polymorphism and its association with clinical mastitis, somatic cell score, and milk production in Polish Holstein-Friesian cattle |
| title_full | L-selectin gene polymorphism and its association with clinical mastitis, somatic cell score, and milk production in Polish Holstein-Friesian cattle |
| title_fullStr | L-selectin gene polymorphism and its association with clinical mastitis, somatic cell score, and milk production in Polish Holstein-Friesian cattle |
| title_full_unstemmed | L-selectin gene polymorphism and its association with clinical mastitis, somatic cell score, and milk production in Polish Holstein-Friesian cattle |
| title_short | L-selectin gene polymorphism and its association with clinical mastitis, somatic cell score, and milk production in Polish Holstein-Friesian cattle |
| title_sort | l selectin gene polymorphism and its association with clinical mastitis somatic cell score and milk production in polish holstein friesian cattle |
| topic | sell udder inflammation milk production traits cows |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201807-0002_l-selectin-gene-polymorphism-and-its-association-with-clinical-mastitis-somatic-cell-score-and-milk-productio.php |
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