Characterizing an engineered carotenoid-producing yeast as an anti-stress chassis for building cell factories
Abstract Background A microorganism engineered for non-native tasks may suffer stresses it never met before. Therefore, we examined whether a Kluyveromyces marxianus strain engineered with a carotenoid biosynthesis pathway can serve as an anti-stress chassis for building cell factories. Results Caro...
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| Format: | Article |
| Language: | English |
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BMC
2019-09-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12934-019-1205-y |
| _version_ | 1818994026556162048 |
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| author | Hsien-Lin Liu Jui-Jen Chang Caroline Thia Yu-Ju Lin Shou-Chen Lo Chieh-Chen Huang Wen-Hsiung Li |
| author_facet | Hsien-Lin Liu Jui-Jen Chang Caroline Thia Yu-Ju Lin Shou-Chen Lo Chieh-Chen Huang Wen-Hsiung Li |
| author_sort | Hsien-Lin Liu |
| collection | DOAJ |
| description | Abstract Background A microorganism engineered for non-native tasks may suffer stresses it never met before. Therefore, we examined whether a Kluyveromyces marxianus strain engineered with a carotenoid biosynthesis pathway can serve as an anti-stress chassis for building cell factories. Results Carotenoids, a family of antioxidants, are valuable natural products with high commercial potential. We showed that the free radical removal ability of carotenoids can confer the engineered host with a higher tolerance to ethanol, so that it can produce more bio-ethanol than the wild type. Moreover, we found that this engineered strain has improved tolerance to other toxic effects including furfurals, heavy metals such as arsenate (biomass contaminant) and isobutanol (end product). Furthermore, the enhanced ethanol tolerance of the host can be applied to bioconversion of a natural medicine that needs to use ethanol as the delivery solvent of hydrophobic precursors. The result suggested that the engineered yeast showed enhanced tolerance to ethanol-dissolved hydrophobic 10-deacetylbaccatin III, which is considered a sustainable precursor for paclitaxel (taxol) bioconversion. Conclusions The stress tolerances of the engineered yeast strain showed tolerance to several toxins, so it may serve as a chassis for cell factories to produce target products, and the co-production of carotenoids may make the biorefinary more cost-effective. |
| first_indexed | 2024-12-20T20:51:24Z |
| format | Article |
| id | doaj.art-1fc35b2099424b36a10c35d91a49fc53 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-20T20:51:24Z |
| publishDate | 2019-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-1fc35b2099424b36a10c35d91a49fc532022-12-21T19:26:54ZengBMCMicrobial Cell Factories1475-28592019-09-011811910.1186/s12934-019-1205-yCharacterizing an engineered carotenoid-producing yeast as an anti-stress chassis for building cell factoriesHsien-Lin Liu0Jui-Jen Chang1Caroline Thia2Yu-Ju Lin3Shou-Chen Lo4Chieh-Chen Huang5Wen-Hsiung Li6Ph.D. Program in Microbial Genomics, National Chung Hsing University and Academia SinicaDepartment of Medical Research, China Medical University HospitalDepartment of Life Sciences, National Chung Hsing UniversityBiodiversity Research Center, Academia SinicaDepartment of Life Sciences, National Chung Hsing UniversityPh.D. Program in Microbial Genomics, National Chung Hsing University and Academia SinicaPh.D. Program in Microbial Genomics, National Chung Hsing University and Academia SinicaAbstract Background A microorganism engineered for non-native tasks may suffer stresses it never met before. Therefore, we examined whether a Kluyveromyces marxianus strain engineered with a carotenoid biosynthesis pathway can serve as an anti-stress chassis for building cell factories. Results Carotenoids, a family of antioxidants, are valuable natural products with high commercial potential. We showed that the free radical removal ability of carotenoids can confer the engineered host with a higher tolerance to ethanol, so that it can produce more bio-ethanol than the wild type. Moreover, we found that this engineered strain has improved tolerance to other toxic effects including furfurals, heavy metals such as arsenate (biomass contaminant) and isobutanol (end product). Furthermore, the enhanced ethanol tolerance of the host can be applied to bioconversion of a natural medicine that needs to use ethanol as the delivery solvent of hydrophobic precursors. The result suggested that the engineered yeast showed enhanced tolerance to ethanol-dissolved hydrophobic 10-deacetylbaccatin III, which is considered a sustainable precursor for paclitaxel (taxol) bioconversion. Conclusions The stress tolerances of the engineered yeast strain showed tolerance to several toxins, so it may serve as a chassis for cell factories to produce target products, and the co-production of carotenoids may make the biorefinary more cost-effective.http://link.springer.com/article/10.1186/s12934-019-1205-yCarotenoidsToxinsBio-ethanolAnti-stress10-deacetylbaccatin IIIAnti-stress |
| spellingShingle | Hsien-Lin Liu Jui-Jen Chang Caroline Thia Yu-Ju Lin Shou-Chen Lo Chieh-Chen Huang Wen-Hsiung Li Characterizing an engineered carotenoid-producing yeast as an anti-stress chassis for building cell factories Microbial Cell Factories Carotenoids Toxins Bio-ethanol Anti-stress 10-deacetylbaccatin III Anti-stress |
| title | Characterizing an engineered carotenoid-producing yeast as an anti-stress chassis for building cell factories |
| title_full | Characterizing an engineered carotenoid-producing yeast as an anti-stress chassis for building cell factories |
| title_fullStr | Characterizing an engineered carotenoid-producing yeast as an anti-stress chassis for building cell factories |
| title_full_unstemmed | Characterizing an engineered carotenoid-producing yeast as an anti-stress chassis for building cell factories |
| title_short | Characterizing an engineered carotenoid-producing yeast as an anti-stress chassis for building cell factories |
| title_sort | characterizing an engineered carotenoid producing yeast as an anti stress chassis for building cell factories |
| topic | Carotenoids Toxins Bio-ethanol Anti-stress 10-deacetylbaccatin III Anti-stress |
| url | http://link.springer.com/article/10.1186/s12934-019-1205-y |
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