EVALUATION OF THE EFFECT OF SPUTUM STORAGE CONDITIONS ON THE VITAL PROPERTIES OF TUBERCULOUS MYCOBACTERIA
Goal of the study: to enhance efficiency of laboratory diagnostics of tuberculous infection through investigating the effect of various storage conditions of samples on the growth properties of Tuberculosis complex mycobacteria.Materials and Methods. 2058 samples of sputum collected by coughing were...
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| Format: | Article |
| Language: | Russian |
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New Terra Publishing House
2017-01-01
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| Series: | Туберкулез и болезни лёгких |
| Subjects: | |
| Online Access: | https://www.tibl-journal.com/jour/article/view/965 |
| _version_ | 1797696660560674816 |
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| author | Yu. D. Rodionova O. A. Gusyakova A. V. Lyamin E. A. Borodulina A. V. Kozlov |
| author_facet | Yu. D. Rodionova O. A. Gusyakova A. V. Lyamin E. A. Borodulina A. V. Kozlov |
| author_sort | Yu. D. Rodionova |
| collection | DOAJ |
| description | Goal of the study: to enhance efficiency of laboratory diagnostics of tuberculous infection through investigating the effect of various storage conditions of samples on the growth properties of Tuberculosis complex mycobacteria.Materials and Methods. 2058 samples of sputum collected by coughing were examined. All sputum samples were aliquoted into 5 parts stored under various conditions: in the fridge under +7°С for 2 hours; frozen for 7 days; by room indoor temperature for 48-72 hours; with the use preservative 10% solution of triple-substituted natrium phosphate for 48 hours; in the fridge under +7°С for 2 hours with consequent treatment by 1% solution of N-acetyl-L-cysteine.Results. The most optimal sputum storage conditions are freezing by -20°С, providing maximum safety of mycobacteria and minimum contamination of the samples with foreign bacteria. Sputum storage by indoor room temperature for 2-3 days reduces the number of positive results of cultures on nutritive media. Using 10% solution of triple-substituted natrium phosphate provides high positive results of bacterioscopic and cultural testing techniques. Time prolongation for biomaterials storage in the preservative solution for more than 72 hours results in the death tuberculous mycobacteria. |
| first_indexed | 2024-03-12T03:29:34Z |
| format | Article |
| id | doaj.art-1fc590c72f4a48cdabd8bfb96386cdbc |
| institution | Directory Open Access Journal |
| issn | 2075-1230 2542-1506 |
| language | Russian |
| last_indexed | 2024-03-12T03:29:34Z |
| publishDate | 2017-01-01 |
| publisher | New Terra Publishing House |
| record_format | Article |
| series | Туберкулез и болезни лёгких |
| spelling | doaj.art-1fc590c72f4a48cdabd8bfb96386cdbc2023-09-03T13:29:12ZrusNew Terra Publishing HouseТуберкулез и болезни лёгких2075-12302542-15062017-01-01951424610.21292/2075-1230-2017-95-1-42-46965EVALUATION OF THE EFFECT OF SPUTUM STORAGE CONDITIONS ON THE VITAL PROPERTIES OF TUBERCULOUS MYCOBACTERIAYu. D. Rodionova0O. A. Gusyakova1A. V. Lyamin2E. A. Borodulina3A. V. Kozlov4ГБОУ ВПО «Самарский государственный медицинский университет» МЗ РФГБОУ ВПО «Самарский государственный медицинский университет» МЗ РФГБОУ ВПО «Самарский государственный медицинский университет» МЗ РФГБОУ ВПО «Самарский государственный медицинский университет» МЗ РФГБОУ ВПО «Самарский государственный медицинский университет» МЗ РФGoal of the study: to enhance efficiency of laboratory diagnostics of tuberculous infection through investigating the effect of various storage conditions of samples on the growth properties of Tuberculosis complex mycobacteria.Materials and Methods. 2058 samples of sputum collected by coughing were examined. All sputum samples were aliquoted into 5 parts stored under various conditions: in the fridge under +7°С for 2 hours; frozen for 7 days; by room indoor temperature for 48-72 hours; with the use preservative 10% solution of triple-substituted natrium phosphate for 48 hours; in the fridge under +7°С for 2 hours with consequent treatment by 1% solution of N-acetyl-L-cysteine.Results. The most optimal sputum storage conditions are freezing by -20°С, providing maximum safety of mycobacteria and minimum contamination of the samples with foreign bacteria. Sputum storage by indoor room temperature for 2-3 days reduces the number of positive results of cultures on nutritive media. Using 10% solution of triple-substituted natrium phosphate provides high positive results of bacterioscopic and cultural testing techniques. Time prolongation for biomaterials storage in the preservative solution for more than 72 hours results in the death tuberculous mycobacteria.https://www.tibl-journal.com/jour/article/view/965условия хранения мокротыростовые свойствамикобактерии туберкулезапитательная средалабораторная диагностика |
| spellingShingle | Yu. D. Rodionova O. A. Gusyakova A. V. Lyamin E. A. Borodulina A. V. Kozlov EVALUATION OF THE EFFECT OF SPUTUM STORAGE CONDITIONS ON THE VITAL PROPERTIES OF TUBERCULOUS MYCOBACTERIA Туберкулез и болезни лёгких условия хранения мокроты ростовые свойства микобактерии туберкулеза питательная среда лабораторная диагностика |
| title | EVALUATION OF THE EFFECT OF SPUTUM STORAGE CONDITIONS ON THE VITAL PROPERTIES OF TUBERCULOUS MYCOBACTERIA |
| title_full | EVALUATION OF THE EFFECT OF SPUTUM STORAGE CONDITIONS ON THE VITAL PROPERTIES OF TUBERCULOUS MYCOBACTERIA |
| title_fullStr | EVALUATION OF THE EFFECT OF SPUTUM STORAGE CONDITIONS ON THE VITAL PROPERTIES OF TUBERCULOUS MYCOBACTERIA |
| title_full_unstemmed | EVALUATION OF THE EFFECT OF SPUTUM STORAGE CONDITIONS ON THE VITAL PROPERTIES OF TUBERCULOUS MYCOBACTERIA |
| title_short | EVALUATION OF THE EFFECT OF SPUTUM STORAGE CONDITIONS ON THE VITAL PROPERTIES OF TUBERCULOUS MYCOBACTERIA |
| title_sort | evaluation of the effect of sputum storage conditions on the vital properties of tuberculous mycobacteria |
| topic | условия хранения мокроты ростовые свойства микобактерии туберкулеза питательная среда лабораторная диагностика |
| url | https://www.tibl-journal.com/jour/article/view/965 |
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