Analysis of glutathione-S-transferases from larvae of Galleria mellonella (Lepidoptera, Pyralidae) with potential alkaloid detoxification function
The greater wax moth, Galleria mellonella, is a global pest for beehives, doing damage in the larval stage. Although a significant number of studies have reported on larvae and adults, to date no effective pest control has been implemented. In this study, we tested larval resistance to alkaloids fro...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.989006/full |
| _version_ | 1798002203986755584 |
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| author | Herbert Venthur Herbert Venthur Paula Lizana Loreto Manosalva Valentina Rojas Ricardo Godoy Adonis Rocha Iván Aguilera Rubén Palma-Millanao Victor Fajardo Andrés Quiroz Andrés Quiroz Ana Mutis Ana Mutis |
| author_facet | Herbert Venthur Herbert Venthur Paula Lizana Loreto Manosalva Valentina Rojas Ricardo Godoy Adonis Rocha Iván Aguilera Rubén Palma-Millanao Victor Fajardo Andrés Quiroz Andrés Quiroz Ana Mutis Ana Mutis |
| author_sort | Herbert Venthur |
| collection | DOAJ |
| description | The greater wax moth, Galleria mellonella, is a global pest for beehives, doing damage in the larval stage. Although a significant number of studies have reported on larvae and adults, to date no effective pest control has been implemented. In this study, we tested larval resistance to alkaloids from Berberis microphylla, and the objective was to identify enzymes that participate in alkaloid detoxification through enzymatic assays, bioinformatics analysis and qRT-PCR. Findings suggest glutathione-S-transferases (GSTs), from an increased metabolic mechanism, are responsible for alkaloid detoxification rather than cytochrome P450 (CYP), carboxylesterases (CarE). A bioinformatics analysis from transcriptome data revealed 22 GSTs present in both G. mellonella larvae and adults. The qRT-PCR experiments corroborated the presence of the 22 GSTs in larvae, where GST8 and GST20 stood out with the highest expression after berberine treatment. Structural information around GST8 and GST20 suggests that GST8 could bind berberine stronger than GST20. These findings represent an important advance in the study of detoxification enzymes in G. mellonella, expanding the role of delta-class GSTs towards alkaloids. Likewise, GST inhibition by alkaloid analogs is proposed in the framework of integrated pest management strategies. |
| first_indexed | 2024-04-11T11:48:24Z |
| format | Article |
| id | doaj.art-1fcb3bd879f5452c84d48b740e95dd02 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-11T11:48:24Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-1fcb3bd879f5452c84d48b740e95dd022022-12-22T04:25:26ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-09-011310.3389/fphys.2022.989006989006Analysis of glutathione-S-transferases from larvae of Galleria mellonella (Lepidoptera, Pyralidae) with potential alkaloid detoxification functionHerbert Venthur0Herbert Venthur1Paula Lizana2Loreto Manosalva3Valentina Rojas4Ricardo Godoy5Adonis Rocha6Iván Aguilera7Rubén Palma-Millanao8Victor Fajardo9Andrés Quiroz10Andrés Quiroz11Ana Mutis12Ana Mutis13Departamento de Ciencias Químicas y Recursos Naturales, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Temuco, ChileCentro de Investigación Biotecnológica Aplicada al Medio Ambiente, CIBAMA, Universidad de La Frontera, Temuco, ChilePrograma de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, Temuco, ChileFacultad de Ciencias, Universidad de Magallanes, Punta Arenas, ChilePrograma de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, Temuco, ChilePrograma de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, Temuco, ChileCarrera de Bioquímica, Departamento de Ciencias Químicas y Recursos Naturales, Universidad de La Frontera, Temuco, ChileCarrera de Bioquímica, Departamento de Ciencias Químicas y Recursos Naturales, Universidad de La Frontera, Temuco, ChileLaboratorio de Ciencias de Insectos, Instituto de Investigaciones Agropecuarias, INIA, Vilcún, ChileFacultad de Ciencias, Universidad de Magallanes, Punta Arenas, ChileDepartamento de Ciencias Químicas y Recursos Naturales, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Temuco, ChileCentro de Investigación Biotecnológica Aplicada al Medio Ambiente, CIBAMA, Universidad de La Frontera, Temuco, ChileDepartamento de Ciencias Químicas y Recursos Naturales, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Temuco, ChileCentro de Investigación Biotecnológica Aplicada al Medio Ambiente, CIBAMA, Universidad de La Frontera, Temuco, ChileThe greater wax moth, Galleria mellonella, is a global pest for beehives, doing damage in the larval stage. Although a significant number of studies have reported on larvae and adults, to date no effective pest control has been implemented. In this study, we tested larval resistance to alkaloids from Berberis microphylla, and the objective was to identify enzymes that participate in alkaloid detoxification through enzymatic assays, bioinformatics analysis and qRT-PCR. Findings suggest glutathione-S-transferases (GSTs), from an increased metabolic mechanism, are responsible for alkaloid detoxification rather than cytochrome P450 (CYP), carboxylesterases (CarE). A bioinformatics analysis from transcriptome data revealed 22 GSTs present in both G. mellonella larvae and adults. The qRT-PCR experiments corroborated the presence of the 22 GSTs in larvae, where GST8 and GST20 stood out with the highest expression after berberine treatment. Structural information around GST8 and GST20 suggests that GST8 could bind berberine stronger than GST20. These findings represent an important advance in the study of detoxification enzymes in G. mellonella, expanding the role of delta-class GSTs towards alkaloids. Likewise, GST inhibition by alkaloid analogs is proposed in the framework of integrated pest management strategies.https://www.frontiersin.org/articles/10.3389/fphys.2022.989006/fullpest controlenzymatic activityGalleria mellonellaalkaloidsglutathione-S-transferase (GST) |
| spellingShingle | Herbert Venthur Herbert Venthur Paula Lizana Loreto Manosalva Valentina Rojas Ricardo Godoy Adonis Rocha Iván Aguilera Rubén Palma-Millanao Victor Fajardo Andrés Quiroz Andrés Quiroz Ana Mutis Ana Mutis Analysis of glutathione-S-transferases from larvae of Galleria mellonella (Lepidoptera, Pyralidae) with potential alkaloid detoxification function Frontiers in Physiology pest control enzymatic activity Galleria mellonella alkaloids glutathione-S-transferase (GST) |
| title | Analysis of glutathione-S-transferases from larvae of Galleria mellonella (Lepidoptera, Pyralidae) with potential alkaloid detoxification function |
| title_full | Analysis of glutathione-S-transferases from larvae of Galleria mellonella (Lepidoptera, Pyralidae) with potential alkaloid detoxification function |
| title_fullStr | Analysis of glutathione-S-transferases from larvae of Galleria mellonella (Lepidoptera, Pyralidae) with potential alkaloid detoxification function |
| title_full_unstemmed | Analysis of glutathione-S-transferases from larvae of Galleria mellonella (Lepidoptera, Pyralidae) with potential alkaloid detoxification function |
| title_short | Analysis of glutathione-S-transferases from larvae of Galleria mellonella (Lepidoptera, Pyralidae) with potential alkaloid detoxification function |
| title_sort | analysis of glutathione s transferases from larvae of galleria mellonella lepidoptera pyralidae with potential alkaloid detoxification function |
| topic | pest control enzymatic activity Galleria mellonella alkaloids glutathione-S-transferase (GST) |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.989006/full |
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