Comparative Analysis of the Lignification Process of Two Bamboo Shoots Stored at Room Temperature
Two types of bamboo shoots, high bamboo (<i>Phyllostachys prominens</i>) shoots (HBSes) and moso bamboo (<i>Phyllostachys edulis</i>) shoots (MBSes), underwent a fast post-harvest lignification process under room temperature storage. To explore the mechanism of lignification...
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MDPI AG
2020-10-01
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| Serija: | Plants |
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| Online pristup: | https://www.mdpi.com/2223-7747/9/10/1399 |
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| author | Zuying Zhang Changtao Li Hui Zhang Yeqing Ying Yuanyuan Hu Lili Song |
| author_facet | Zuying Zhang Changtao Li Hui Zhang Yeqing Ying Yuanyuan Hu Lili Song |
| author_sort | Zuying Zhang |
| collection | DOAJ |
| description | Two types of bamboo shoots, high bamboo (<i>Phyllostachys prominens</i>) shoots (HBSes) and moso bamboo (<i>Phyllostachys edulis</i>) shoots (MBSes), underwent a fast post-harvest lignification process under room temperature storage. To explore the mechanism of lignification in two types of bamboo shoots after post-harvest during room temperature storage, the measurement of cell wall polymers (lignin and cellulose) and enzyme activities of phenylalanine ammonialyase (PAL) and peroxidase (POD), and relative expression of related transcription networks factors (TFs) were performed. The results suggested that the lignification process in HBSes is faster than that in MBSes because of incremental increase in lignin and cellulose contents within 6 days and the shorter shelf-life. Additionally, compared with the expression pattern of lignification-related TFs and correlation analysis of lignin and cellulose contents, <i>MYB20</i>, <i>MYB43</i>, <i>MYB85</i> could function positively in the lignification process of two types of bamboo shoots. A negative regulator, <i>KNAT7,</i> could negatively regulate the lignin biosynthesis in two types of bamboo shoots. In addition, <i>MYB63</i> could function positively in HBSes, and <i>NST1</i> could function negatively in MBSes. Notably, <i>MYB42</i> may function differently in the two types of bamboo shoots, that is, a positive regulator in HBSes, but a negative regulator in MBSes. Transcription networks provide a comprehensive analysis to explore the mechanism of lignification in two types of bamboo shoots after post-harvest during room temperature storage. These results suggest that the lignification of bamboo shoots was mainly due to the increased activity of POD, higher expression levels of <i>MYB20</i>, <i>MYB43, MYB63,</i> and <i>MYB85</i> genes, and lower expression levels of <i>KNAT7</i> and <i>NST1</i> genes, and the lignification process of HBSes and MBSes had significant differences. |
| first_indexed | 2024-03-10T15:27:17Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T15:27:17Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-1fcd95b0bd264a4ebe510e50cd16b2cc2023-11-20T17:54:14ZengMDPI AGPlants2223-77472020-10-01910139910.3390/plants9101399Comparative Analysis of the Lignification Process of Two Bamboo Shoots Stored at Room TemperatureZuying Zhang0Changtao Li1Hui Zhang2Yeqing Ying3Yuanyuan Hu4Lili Song5State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an 311300, ChinaTwo types of bamboo shoots, high bamboo (<i>Phyllostachys prominens</i>) shoots (HBSes) and moso bamboo (<i>Phyllostachys edulis</i>) shoots (MBSes), underwent a fast post-harvest lignification process under room temperature storage. To explore the mechanism of lignification in two types of bamboo shoots after post-harvest during room temperature storage, the measurement of cell wall polymers (lignin and cellulose) and enzyme activities of phenylalanine ammonialyase (PAL) and peroxidase (POD), and relative expression of related transcription networks factors (TFs) were performed. The results suggested that the lignification process in HBSes is faster than that in MBSes because of incremental increase in lignin and cellulose contents within 6 days and the shorter shelf-life. Additionally, compared with the expression pattern of lignification-related TFs and correlation analysis of lignin and cellulose contents, <i>MYB20</i>, <i>MYB43</i>, <i>MYB85</i> could function positively in the lignification process of two types of bamboo shoots. A negative regulator, <i>KNAT7,</i> could negatively regulate the lignin biosynthesis in two types of bamboo shoots. In addition, <i>MYB63</i> could function positively in HBSes, and <i>NST1</i> could function negatively in MBSes. Notably, <i>MYB42</i> may function differently in the two types of bamboo shoots, that is, a positive regulator in HBSes, but a negative regulator in MBSes. Transcription networks provide a comprehensive analysis to explore the mechanism of lignification in two types of bamboo shoots after post-harvest during room temperature storage. These results suggest that the lignification of bamboo shoots was mainly due to the increased activity of POD, higher expression levels of <i>MYB20</i>, <i>MYB43, MYB63,</i> and <i>MYB85</i> genes, and lower expression levels of <i>KNAT7</i> and <i>NST1</i> genes, and the lignification process of HBSes and MBSes had significant differences.https://www.mdpi.com/2223-7747/9/10/1399high bamboo shootsmoso bamboo shootsroom temperaturelignification processtranscription factorregulation |
| spellingShingle | Zuying Zhang Changtao Li Hui Zhang Yeqing Ying Yuanyuan Hu Lili Song Comparative Analysis of the Lignification Process of Two Bamboo Shoots Stored at Room Temperature Plants high bamboo shoots moso bamboo shoots room temperature lignification process transcription factor regulation |
| title | Comparative Analysis of the Lignification Process of Two Bamboo Shoots Stored at Room Temperature |
| title_full | Comparative Analysis of the Lignification Process of Two Bamboo Shoots Stored at Room Temperature |
| title_fullStr | Comparative Analysis of the Lignification Process of Two Bamboo Shoots Stored at Room Temperature |
| title_full_unstemmed | Comparative Analysis of the Lignification Process of Two Bamboo Shoots Stored at Room Temperature |
| title_short | Comparative Analysis of the Lignification Process of Two Bamboo Shoots Stored at Room Temperature |
| title_sort | comparative analysis of the lignification process of two bamboo shoots stored at room temperature |
| topic | high bamboo shoots moso bamboo shoots room temperature lignification process transcription factor regulation |
| url | https://www.mdpi.com/2223-7747/9/10/1399 |
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