Enhancing Biodegradation of Pyridine with Trehalose Lipid in <i>Rhodococcus pyridinivorans</i> sp. Strain HR-1-Inoculated Microbial Fuel Cell
A Gram-positive exoelectrogen <i>Rhodococcus pyridinivorans</i> sp. strain HR-1 was cultivated from leachate-fed microbial fuel cell (MFC) to study the biodegradation effect of pyridine. In the comparison with mixed cultured MFC, HR-1 presented a remarkable electrical capacity with a max...
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MDPI AG
2023-01-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/2/133 |
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| author | Peng Cheng Muhammad Usman Muhammad Arslan Huanqing Sun Li Zhou Mohamed Gamal El-Din |
| author_facet | Peng Cheng Muhammad Usman Muhammad Arslan Huanqing Sun Li Zhou Mohamed Gamal El-Din |
| author_sort | Peng Cheng |
| collection | DOAJ |
| description | A Gram-positive exoelectrogen <i>Rhodococcus pyridinivorans</i> sp. strain HR-1 was cultivated from leachate-fed microbial fuel cell (MFC) to study the biodegradation effect of pyridine. In the comparison with mixed cultured MFC, HR-1 presented a remarkable electrical capacity with a maximum output of 4.33 W/m<sup>3</sup> under 30 °C in neutral anolyte with 1 g/L acetate as a substrate. Further, HR-1 demonstrated the environmental resistance as a Gram-positive strain. Microbial metabolism was evident at pH between 5–9 and temperature in the range of 20–40 °C, whereas optimal condition for pyridine degradation was observed at 30 °C. This is the first study reporting the degradation of pyridine in the bio-electrochemical system that achieved a 42% ± 5% degradation rate in a full operation cycle at 2 g/L of the concentration. Considering the nonnegligible internal resistance of HR-1-inoculated MFC, trehalose lipid was also introduced as a bio-surfactant to reduce the charge transfer obstacle between the microbes and the electrodes. The surface morphology illustrated that the strain had a plump shape with a high specific area. Accordingly, bio-surfactant addition promoted the anode biomass (1.2 ± 0.1 mg/cm<sup>2</sup> to 1.7 ± 0.2 mg/cm<sup>2</sup>) and achieved a higher degradation rate (68% ± 4%). The feasibility of electrochemical disposal on pyridine and eminent adaptability of strain sp. HR-1 as a Gram-positive exoelectrogen makes MFC a practical approach for real application. |
| first_indexed | 2024-03-11T08:50:32Z |
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| language | English |
| last_indexed | 2024-03-11T08:50:32Z |
| publishDate | 2023-01-01 |
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| series | Fermentation |
| spelling | doaj.art-1fcec399384f473eac5c953005d183ed2023-11-16T20:25:55ZengMDPI AGFermentation2311-56372023-01-019213310.3390/fermentation9020133Enhancing Biodegradation of Pyridine with Trehalose Lipid in <i>Rhodococcus pyridinivorans</i> sp. Strain HR-1-Inoculated Microbial Fuel CellPeng Cheng0Muhammad Usman1Muhammad Arslan2Huanqing Sun3Li Zhou4Mohamed Gamal El-Din5Institute of Environmental Research at Greater Bay Area, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou 510006, ChinaDepartment of Civil and Environmental Engineering, University of Alberta, Edmonton, AL T6G 1H9, CanadaDepartment of Civil and Environmental Engineering, University of Alberta, Edmonton, AL T6G 1H9, CanadaDepartment of Biology, Hengshui University, Hengshui 053000, ChinaInstitute of Environmental Research at Greater Bay Area, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou 510006, ChinaDepartment of Civil and Environmental Engineering, University of Alberta, Edmonton, AL T6G 1H9, CanadaA Gram-positive exoelectrogen <i>Rhodococcus pyridinivorans</i> sp. strain HR-1 was cultivated from leachate-fed microbial fuel cell (MFC) to study the biodegradation effect of pyridine. In the comparison with mixed cultured MFC, HR-1 presented a remarkable electrical capacity with a maximum output of 4.33 W/m<sup>3</sup> under 30 °C in neutral anolyte with 1 g/L acetate as a substrate. Further, HR-1 demonstrated the environmental resistance as a Gram-positive strain. Microbial metabolism was evident at pH between 5–9 and temperature in the range of 20–40 °C, whereas optimal condition for pyridine degradation was observed at 30 °C. This is the first study reporting the degradation of pyridine in the bio-electrochemical system that achieved a 42% ± 5% degradation rate in a full operation cycle at 2 g/L of the concentration. Considering the nonnegligible internal resistance of HR-1-inoculated MFC, trehalose lipid was also introduced as a bio-surfactant to reduce the charge transfer obstacle between the microbes and the electrodes. The surface morphology illustrated that the strain had a plump shape with a high specific area. Accordingly, bio-surfactant addition promoted the anode biomass (1.2 ± 0.1 mg/cm<sup>2</sup> to 1.7 ± 0.2 mg/cm<sup>2</sup>) and achieved a higher degradation rate (68% ± 4%). The feasibility of electrochemical disposal on pyridine and eminent adaptability of strain sp. HR-1 as a Gram-positive exoelectrogen makes MFC a practical approach for real application.https://www.mdpi.com/2311-5637/9/2/133microbial fuel cellsgram-positive exoelectrogenpyridinetrehalose lipid<i>Rhodococcus pyridinivorans</i> |
| spellingShingle | Peng Cheng Muhammad Usman Muhammad Arslan Huanqing Sun Li Zhou Mohamed Gamal El-Din Enhancing Biodegradation of Pyridine with Trehalose Lipid in <i>Rhodococcus pyridinivorans</i> sp. Strain HR-1-Inoculated Microbial Fuel Cell Fermentation microbial fuel cells gram-positive exoelectrogen pyridine trehalose lipid <i>Rhodococcus pyridinivorans</i> |
| title | Enhancing Biodegradation of Pyridine with Trehalose Lipid in <i>Rhodococcus pyridinivorans</i> sp. Strain HR-1-Inoculated Microbial Fuel Cell |
| title_full | Enhancing Biodegradation of Pyridine with Trehalose Lipid in <i>Rhodococcus pyridinivorans</i> sp. Strain HR-1-Inoculated Microbial Fuel Cell |
| title_fullStr | Enhancing Biodegradation of Pyridine with Trehalose Lipid in <i>Rhodococcus pyridinivorans</i> sp. Strain HR-1-Inoculated Microbial Fuel Cell |
| title_full_unstemmed | Enhancing Biodegradation of Pyridine with Trehalose Lipid in <i>Rhodococcus pyridinivorans</i> sp. Strain HR-1-Inoculated Microbial Fuel Cell |
| title_short | Enhancing Biodegradation of Pyridine with Trehalose Lipid in <i>Rhodococcus pyridinivorans</i> sp. Strain HR-1-Inoculated Microbial Fuel Cell |
| title_sort | enhancing biodegradation of pyridine with trehalose lipid in i rhodococcus pyridinivorans i sp strain hr 1 inoculated microbial fuel cell |
| topic | microbial fuel cells gram-positive exoelectrogen pyridine trehalose lipid <i>Rhodococcus pyridinivorans</i> |
| url | https://www.mdpi.com/2311-5637/9/2/133 |
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