| Summary: | This study was carried out to investigate effects of copper sulphate (CuSO4) additive tosemen extenders on sperm parameters:progressive motility, viability, membrane integrityand DNA damage, after semen dilution and cryopreservation. Semen samples of 5 buffalobulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. A totalnumber of 25 samples were used in each examination. Sperm progressive motility andviability were measured at 0 (T0), 60 (T1) and 120 (T2) min after diluting semen in tris-citricacid extender containing 0 (control), 0.004, 0.008, 0.016, 0.032 and 0.064 mg L-1CuSO4. Later,semen was diluted in a tris-citric acid-egg yolk-glycerol extender containing the sameamounts ofCuSO4, cooled to 4̊C and kept refrigerated for 4 hr to equilibrate, spermprogressive motility, viability, membrane integrity and DNA damage were estimated. Then,semen was packed in 0.5 mL French straws and frozen in liquid nitrogen. Later, the frozensemen was thawed in 37 ̊C water bath for 30 sec, and the same parameters as well as totalantioxidant capacity (TAC) of the frozen-thawed semen were estimated. The results showedthat copper additive at the rate of 0.032 mg L-1gives a better protection of sperms throughthe process of dilution, equilibration and freeze-thawing than that in control and other Cuconcentrations, while 0.064 mg L-1CuSO4had deleterious effect on the sperm.
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