Comparative Analysis of Gram Stain, Culture and Bacterial Antigen Detection in Cerebrospinal Fluid Samples for Laboratory Diagnosis of Acute Bacterial Meningitis in Pediatric Population in A Tertiary Care Hospital
Acute bacterial meningitis (ABM) is a life threatening infection in children, associated with long term complications and high mortality rate.1,2 Gram staining and culture are routinely used for diagnosis of ABM. Antigen detection by latex agglutination can provide prompt results thereby facilitatin...
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| Format: | Article |
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Journal of Pure and Applied Microbiology
2023-09-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/comparative-analysis-of-gram-stain-culture-and-bacterial-antigen-detection-in-cerebrospinal-fluid-samples-for-laboratory-diagnosis-of-acute-bacterial-meningitis-in-pediatric-population-in-a-tertiary/ |
| _version_ | 1797683392941129728 |
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| author | V. Dillirani J. Jayachitra K. Chandrasekaran T. Monisha |
| author_facet | V. Dillirani J. Jayachitra K. Chandrasekaran T. Monisha |
| author_sort | V. Dillirani |
| collection | DOAJ |
| description | Acute bacterial meningitis (ABM) is a life threatening infection in children, associated with long term complications and high mortality rate.1,2 Gram staining and culture are routinely used for diagnosis of ABM. Antigen detection by latex agglutination can provide prompt results thereby facilitating early initiation of empirical antibiotic treatment. To estimate the proportion of Laboratory confirmed cases among children admitted with clinical suspicion of acute bacterial meningitis in a tertiary care hospital. To compare and analyse the diagnostic efficacy of Culture, Gram stain and antigen detection by Latex agglutination in Cerebrospinal fluid (CSF) samples for laboratory detection of Acute bacterial meningitis. CSF samples from pediatric patients with clinical suspicion of ABM were analysed by Gram stain, culture and Antigen detection by Latex agglutination method. Results were recorded and analysed. Of the 50 clinically suspected cases, 13(26%) were confirmed as Acute bacterial meningitis by laboratory investigations. Among the organisms identified, Streptococcus pneumoniae was the most common isolate in 5(38.46%) cases followed by Neisseria meningitidis, Klebsiella pneumoniae and Acinetobacter baumannii in 2(15.38%) cases each and Escherichia coli and Group B Streptococcus in 1(7.69%) case each. Among the confirmed cases, 7(53%) samples showed culture positivity while Gram stain identified 8(61.53%)cases. Latex agglutination test showed positivity in 9(69.23%) cases. In life threatening infections like acute bacterial meningitis, where early diagnosis and prompt treatment is of utmost importance, Latex agglutination test can provide results within minutes facilitating early initiation of empirical therapy, making it an effective adjunct to gram stain and culture. |
| first_indexed | 2024-03-12T00:14:42Z |
| format | Article |
| id | doaj.art-1fd02be1627f4c29b98f170ab02a0117 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-03-12T00:14:42Z |
| publishDate | 2023-09-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-1fd02be1627f4c29b98f170ab02a01172023-09-15T15:56:27ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2023-09-0117317151721https://doi.org/10.22207/JPAM.17.3.36Comparative Analysis of Gram Stain, Culture and Bacterial Antigen Detection in Cerebrospinal Fluid Samples for Laboratory Diagnosis of Acute Bacterial Meningitis in Pediatric Population in A Tertiary Care HospitalV. Dilliranihttps://orcid.org/0000-0001-5004-7606 J. Jayachitrahttps://orcid.org/0009-0005-3338-3938K. Chandrasekaranhttps://orcid.org/0009-0007-2849-6748T. MonishaAcute bacterial meningitis (ABM) is a life threatening infection in children, associated with long term complications and high mortality rate.1,2 Gram staining and culture are routinely used for diagnosis of ABM. Antigen detection by latex agglutination can provide prompt results thereby facilitating early initiation of empirical antibiotic treatment. To estimate the proportion of Laboratory confirmed cases among children admitted with clinical suspicion of acute bacterial meningitis in a tertiary care hospital. To compare and analyse the diagnostic efficacy of Culture, Gram stain and antigen detection by Latex agglutination in Cerebrospinal fluid (CSF) samples for laboratory detection of Acute bacterial meningitis. CSF samples from pediatric patients with clinical suspicion of ABM were analysed by Gram stain, culture and Antigen detection by Latex agglutination method. Results were recorded and analysed. Of the 50 clinically suspected cases, 13(26%) were confirmed as Acute bacterial meningitis by laboratory investigations. Among the organisms identified, Streptococcus pneumoniae was the most common isolate in 5(38.46%) cases followed by Neisseria meningitidis, Klebsiella pneumoniae and Acinetobacter baumannii in 2(15.38%) cases each and Escherichia coli and Group B Streptococcus in 1(7.69%) case each. Among the confirmed cases, 7(53%) samples showed culture positivity while Gram stain identified 8(61.53%)cases. Latex agglutination test showed positivity in 9(69.23%) cases. In life threatening infections like acute bacterial meningitis, where early diagnosis and prompt treatment is of utmost importance, Latex agglutination test can provide results within minutes facilitating early initiation of empirical therapy, making it an effective adjunct to gram stain and culture.https://microbiologyjournal.org/comparative-analysis-of-gram-stain-culture-and-bacterial-antigen-detection-in-cerebrospinal-fluid-samples-for-laboratory-diagnosis-of-acute-bacterial-meningitis-in-pediatric-population-in-a-tertiary/laboratory diagnosisacute bacterial meningitisgram stainculturerapid diagnosislatex agglutination method |
| spellingShingle | V. Dillirani J. Jayachitra K. Chandrasekaran T. Monisha Comparative Analysis of Gram Stain, Culture and Bacterial Antigen Detection in Cerebrospinal Fluid Samples for Laboratory Diagnosis of Acute Bacterial Meningitis in Pediatric Population in A Tertiary Care Hospital Journal of Pure and Applied Microbiology laboratory diagnosis acute bacterial meningitis gram stain culture rapid diagnosis latex agglutination method |
| title | Comparative Analysis of Gram Stain, Culture and Bacterial Antigen Detection in Cerebrospinal Fluid Samples for Laboratory Diagnosis of Acute Bacterial Meningitis in Pediatric Population in A Tertiary Care Hospital |
| title_full | Comparative Analysis of Gram Stain, Culture and Bacterial Antigen Detection in Cerebrospinal Fluid Samples for Laboratory Diagnosis of Acute Bacterial Meningitis in Pediatric Population in A Tertiary Care Hospital |
| title_fullStr | Comparative Analysis of Gram Stain, Culture and Bacterial Antigen Detection in Cerebrospinal Fluid Samples for Laboratory Diagnosis of Acute Bacterial Meningitis in Pediatric Population in A Tertiary Care Hospital |
| title_full_unstemmed | Comparative Analysis of Gram Stain, Culture and Bacterial Antigen Detection in Cerebrospinal Fluid Samples for Laboratory Diagnosis of Acute Bacterial Meningitis in Pediatric Population in A Tertiary Care Hospital |
| title_short | Comparative Analysis of Gram Stain, Culture and Bacterial Antigen Detection in Cerebrospinal Fluid Samples for Laboratory Diagnosis of Acute Bacterial Meningitis in Pediatric Population in A Tertiary Care Hospital |
| title_sort | comparative analysis of gram stain culture and bacterial antigen detection in cerebrospinal fluid samples for laboratory diagnosis of acute bacterial meningitis in pediatric population in a tertiary care hospital |
| topic | laboratory diagnosis acute bacterial meningitis gram stain culture rapid diagnosis latex agglutination method |
| url | https://microbiologyjournal.org/comparative-analysis-of-gram-stain-culture-and-bacterial-antigen-detection-in-cerebrospinal-fluid-samples-for-laboratory-diagnosis-of-acute-bacterial-meningitis-in-pediatric-population-in-a-tertiary/ |
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