Novel chemical- and protein-mediated methods for glucosamine detection
We describe two novel approaches for the determination of glucosamine (GlcN). The first approach is based on the chemical derivatization of GlcN with the non-fluorophor 1,3-diphenyl-1,3-propanedione (DPPD), which results in a condensation product with interesting fluorescent properties. The obtained...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Maximum Academic Press
2022-01-01
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| Series: | Food Materials Research |
| Subjects: | |
| Online Access: | https://www.maxapress.com/article/doi/10.48130/FMR-2022-0019 |
| _version_ | 1797293213364518912 |
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| author | Linshu Chen Pedro Laborda Zhipeng Cai Andrew Kevin Hagan Aimin Lu Josef Voglmeir Li Liu |
| author_facet | Linshu Chen Pedro Laborda Zhipeng Cai Andrew Kevin Hagan Aimin Lu Josef Voglmeir Li Liu |
| author_sort | Linshu Chen |
| collection | DOAJ |
| description | We describe two novel approaches for the determination of glucosamine (GlcN). The first approach is based on the chemical derivatization of GlcN with the non-fluorophor 1,3-diphenyl-1,3-propanedione (DPPD), which results in a condensation product with interesting fluorescent properties. The obtained compound was isolated by silica-gel chromatography and its structure elucidated by NMR and mass spectrometry. The second approach is based on a previously undescribed sensitivity of the enzyme glucosamine-6-phosphate deaminase (GPDA) towards GlcN, which resulted in the precipitation of the enzyme. Using a rational enzyme engineering approach and both liquid-based and plate-based screening methods, mutational GPDA variants with significantly improved precipitation properties were identified and characterized. These novel glucosamine detection methods may be a useful addition to the repertoire of currently available glucosamine detection sensors. |
| first_indexed | 2024-03-07T20:09:38Z |
| format | Article |
| id | doaj.art-1fd11ff665944569945915694aaeb920 |
| institution | Directory Open Access Journal |
| issn | 2771-4683 |
| language | English |
| last_indexed | 2024-03-07T20:09:38Z |
| publishDate | 2022-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Food Materials Research |
| spelling | doaj.art-1fd11ff665944569945915694aaeb9202024-02-28T01:36:04ZengMaximum Academic PressFood Materials Research2771-46832022-01-01211810.48130/FMR-2022-0019FMR-2022-0019Novel chemical- and protein-mediated methods for glucosamine detectionLinshu Chen0Pedro Laborda1Zhipeng Cai2Andrew Kevin Hagan3Aimin Lu4Josef Voglmeir5Li Liu6Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, People's Republic of ChinaGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, People's Republic of ChinaGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, People's Republic of ChinaGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, People's Republic of ChinaCollege of Sciences, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, People's Republic of ChinaGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, People's Republic of ChinaGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, People's Republic of ChinaWe describe two novel approaches for the determination of glucosamine (GlcN). The first approach is based on the chemical derivatization of GlcN with the non-fluorophor 1,3-diphenyl-1,3-propanedione (DPPD), which results in a condensation product with interesting fluorescent properties. The obtained compound was isolated by silica-gel chromatography and its structure elucidated by NMR and mass spectrometry. The second approach is based on a previously undescribed sensitivity of the enzyme glucosamine-6-phosphate deaminase (GPDA) towards GlcN, which resulted in the precipitation of the enzyme. Using a rational enzyme engineering approach and both liquid-based and plate-based screening methods, mutational GPDA variants with significantly improved precipitation properties were identified and characterized. These novel glucosamine detection methods may be a useful addition to the repertoire of currently available glucosamine detection sensors.https://www.maxapress.com/article/doi/10.48130/FMR-2022-0019glucosamine detectionfluorescence labelingprotein engineeringassay developmentdirected evolution |
| spellingShingle | Linshu Chen Pedro Laborda Zhipeng Cai Andrew Kevin Hagan Aimin Lu Josef Voglmeir Li Liu Novel chemical- and protein-mediated methods for glucosamine detection Food Materials Research glucosamine detection fluorescence labeling protein engineering assay development directed evolution |
| title | Novel chemical- and protein-mediated methods for glucosamine detection |
| title_full | Novel chemical- and protein-mediated methods for glucosamine detection |
| title_fullStr | Novel chemical- and protein-mediated methods for glucosamine detection |
| title_full_unstemmed | Novel chemical- and protein-mediated methods for glucosamine detection |
| title_short | Novel chemical- and protein-mediated methods for glucosamine detection |
| title_sort | novel chemical and protein mediated methods for glucosamine detection |
| topic | glucosamine detection fluorescence labeling protein engineering assay development directed evolution |
| url | https://www.maxapress.com/article/doi/10.48130/FMR-2022-0019 |
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