WFDC21P promotes triple-negative breast cancer proliferation and migration through WFDC21P/miR-628/SMAD3 axis
Long non-coding RNAs (lncRNAs) modulate cell proliferation, cycle, and apoptosis. However, the role of lncRNA-WFDC21P in the tumorigenesis of triple-negative breast cancer (TNBC) remains unclear. Results of this study demonstrated that WFDC21P levels significantly increased in TNBC, which was associ...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.1032850/full |
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| author | Yu-Bo Wei Dong-Min Liang Mei-Ling Zhang You-Jie Li Hong-Fang Sun Qin Wang Yan Liang Yan Liang Yan-Mei Li Ran-Ran Wang Zhen-Lin Yang Pingyu Wang Pingyu Wang Shu-Yang Xie Shu-Yang Xie |
| author_facet | Yu-Bo Wei Dong-Min Liang Mei-Ling Zhang You-Jie Li Hong-Fang Sun Qin Wang Yan Liang Yan Liang Yan-Mei Li Ran-Ran Wang Zhen-Lin Yang Pingyu Wang Pingyu Wang Shu-Yang Xie Shu-Yang Xie |
| author_sort | Yu-Bo Wei |
| collection | DOAJ |
| description | Long non-coding RNAs (lncRNAs) modulate cell proliferation, cycle, and apoptosis. However, the role of lncRNA-WFDC21P in the tumorigenesis of triple-negative breast cancer (TNBC) remains unclear. Results of this study demonstrated that WFDC21P levels significantly increased in TNBC, which was associated with the poor survival of patients. WFDC21P overexpression significantly promoted TNBC cell proliferation and metastasis. WFDC21P interacted with miR-628-5p, which further suppressed cell proliferation and metastasis by negatively regulating Smad3-related gene expression. Recovery of miR-628-5p weakened the roles of WFDC21P in promoting the growth and metastasis of TNBC cells. Moreover,N6-methyladenosine (m6A) modification upregulated WFDC21P expression in the TNBC cells. WFDC21P and its m6A levels were increased after methyltransferase like 3 (METTL3) overexpression but reduced after METTL3 silencing. The proliferation and metastasis of TNBC cells were promoted by METTL3 overexpression but suppressed by METTL3 silencing. This study demonstrated the vital roles of WFDC21P and its m6A in regulating the proliferation and metastasis of TNBC cells via the WFDC21P/miR-628/SMAD3 axis. |
| first_indexed | 2024-04-13T22:20:48Z |
| format | Article |
| id | doaj.art-1fd33487cb7f430bbc574bddb427a59b |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-13T22:20:48Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-1fd33487cb7f430bbc574bddb427a59b2022-12-22T02:27:15ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-10-011210.3389/fonc.2022.10328501032850WFDC21P promotes triple-negative breast cancer proliferation and migration through WFDC21P/miR-628/SMAD3 axisYu-Bo Wei0Dong-Min Liang1Mei-Ling Zhang2You-Jie Li3Hong-Fang Sun4Qin Wang5Yan Liang6Yan Liang7Yan-Mei Li8Ran-Ran Wang9Zhen-Lin Yang10Pingyu Wang11Pingyu Wang12Shu-Yang Xie13Shu-Yang Xie14Key Laboratory of Tumor Molecular Biology, Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, ChinaKey Laboratory of Tumor Molecular Biology, Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, ChinaKey Laboratory of Tumor Molecular Biology, Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, ChinaKey Laboratory of Tumor Molecular Biology, Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, ChinaKey Laboratory of Tumor Molecular Biology, Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, ChinaKey Laboratory of Tumor Molecular Biology, Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, ChinaKey Laboratory of Tumor Molecular Biology, Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, ChinaDepartment of Physiology and Pathophysiology, School of Basic Medicine, Qingdao University, Qingdao, Shandong, ChinaDepartment of Immune Rheumatism, Yantaishan Hospital, Yantai, Shandong, ChinaInstitute of Rehabilitation Medicine, School of Rehabilitation Medicine, Binzhou Medical University, Yantai, Shandong, ChinaDepartment of Breast and Thyroid Surgery, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong, ChinaKey Laboratory of Tumor Molecular Biology, Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, ChinaDepartment of Epidemiology, Binzhou Medical University, Yantai, Shandong, ChinaKey Laboratory of Tumor Molecular Biology, Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, ChinaDepartment of Physiology and Pathophysiology, School of Basic Medicine, Qingdao University, Qingdao, Shandong, ChinaLong non-coding RNAs (lncRNAs) modulate cell proliferation, cycle, and apoptosis. However, the role of lncRNA-WFDC21P in the tumorigenesis of triple-negative breast cancer (TNBC) remains unclear. Results of this study demonstrated that WFDC21P levels significantly increased in TNBC, which was associated with the poor survival of patients. WFDC21P overexpression significantly promoted TNBC cell proliferation and metastasis. WFDC21P interacted with miR-628-5p, which further suppressed cell proliferation and metastasis by negatively regulating Smad3-related gene expression. Recovery of miR-628-5p weakened the roles of WFDC21P in promoting the growth and metastasis of TNBC cells. Moreover,N6-methyladenosine (m6A) modification upregulated WFDC21P expression in the TNBC cells. WFDC21P and its m6A levels were increased after methyltransferase like 3 (METTL3) overexpression but reduced after METTL3 silencing. The proliferation and metastasis of TNBC cells were promoted by METTL3 overexpression but suppressed by METTL3 silencing. This study demonstrated the vital roles of WFDC21P and its m6A in regulating the proliferation and metastasis of TNBC cells via the WFDC21P/miR-628/SMAD3 axis.https://www.frontiersin.org/articles/10.3389/fonc.2022.1032850/fullWFDC21PmicroRNAN6-methyladenosineepigeneticstriple-negative breast cancer |
| spellingShingle | Yu-Bo Wei Dong-Min Liang Mei-Ling Zhang You-Jie Li Hong-Fang Sun Qin Wang Yan Liang Yan Liang Yan-Mei Li Ran-Ran Wang Zhen-Lin Yang Pingyu Wang Pingyu Wang Shu-Yang Xie Shu-Yang Xie WFDC21P promotes triple-negative breast cancer proliferation and migration through WFDC21P/miR-628/SMAD3 axis Frontiers in Oncology WFDC21P microRNA N6-methyladenosine epigenetics triple-negative breast cancer |
| title | WFDC21P promotes triple-negative breast cancer proliferation and migration through WFDC21P/miR-628/SMAD3 axis |
| title_full | WFDC21P promotes triple-negative breast cancer proliferation and migration through WFDC21P/miR-628/SMAD3 axis |
| title_fullStr | WFDC21P promotes triple-negative breast cancer proliferation and migration through WFDC21P/miR-628/SMAD3 axis |
| title_full_unstemmed | WFDC21P promotes triple-negative breast cancer proliferation and migration through WFDC21P/miR-628/SMAD3 axis |
| title_short | WFDC21P promotes triple-negative breast cancer proliferation and migration through WFDC21P/miR-628/SMAD3 axis |
| title_sort | wfdc21p promotes triple negative breast cancer proliferation and migration through wfdc21p mir 628 smad3 axis |
| topic | WFDC21P microRNA N6-methyladenosine epigenetics triple-negative breast cancer |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.1032850/full |
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