Identification and determination of effective and immunogenic antigens of Ascaridia galli in poultry reared in Tabriz area
In a trial, in Tabriz area, the ability of an experimental vaccine from the larval homogenate of Ascaridia galli was evaluated during the year 1384. A group of 5 white leghorn hens were immunized twice with 100 micrograms larval homogenate of Ascaridia galli diluted in 1ml PBS and emulsified in 1ml...
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| Format: | Article |
| Language: | fas |
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Islamic Azad University, Tabriz Branch
2007-05-01
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| Series: | Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī |
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| Online Access: | http://jvcp.iaut.ac.ir/article_518631_3a64b7d5e7ce08eb8fcac22966aeb03b.pdf |
| _version_ | 1819074171793047552 |
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| author | Yaghob Gharehdaghi Naser Hoghooghi Rad Parviz Shayan Ali Eslami |
| author_facet | Yaghob Gharehdaghi Naser Hoghooghi Rad Parviz Shayan Ali Eslami |
| author_sort | Yaghob Gharehdaghi |
| collection | DOAJ |
| description | In a trial, in Tabriz area, the ability of an experimental vaccine from the larval homogenate of Ascaridia galli was evaluated during the year 1384. A group of 5 white leghorn hens were immunized twice with 100 micrograms larval homogenate of Ascaridia galli diluted in 1ml PBS and emulsified in 1ml Freund’s adjuvant orally. Booster immunization was also administered orally on day 21 The other group (5 white leghorn hens) received 1 ml PBS emulsified in 1ml of the same adjuvant. On day 33 each hen was administered approximately 10,000 Ascaridia galli infective eggs orally. Blood samples were collected from the animals in a 10 day interval from the first immunization until infective challenge and weekly thereafter until the end of study. Sera were tested by ELISA and Western-blotting. Hens were necropsied 8 weeks post challenge with infective eggs for recovery of the nematodes. Vaccinated hens showed a 76 percent reduction in mean EPG, 74 percent reduction in the average number of male worms, 79 percent reduction in the average number of female worms and 77 percent reduction in the mean number of total worms. Significance in mean optical density of sera in ELISA was noticed (p |
| first_indexed | 2024-12-21T18:05:17Z |
| format | Article |
| id | doaj.art-1fd3d9606f284205a90fc6c7352d9178 |
| institution | Directory Open Access Journal |
| issn | 2322-4746 2476-6984 |
| language | fas |
| last_indexed | 2024-12-21T18:05:17Z |
| publishDate | 2007-05-01 |
| publisher | Islamic Azad University, Tabriz Branch |
| record_format | Article |
| series | Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī |
| spelling | doaj.art-1fd3d9606f284205a90fc6c7352d91782022-12-21T18:54:56ZfasIslamic Azad University, Tabriz BranchĀsīb/shināsī-i Darmāngāhī-i Dāmpizishkī2322-47462476-69842007-05-0111 (1) بهار16518631Identification and determination of effective and immunogenic antigens of Ascaridia galli in poultry reared in Tabriz areaYaghob Gharehdaghi0Naser Hoghooghi Rad1Parviz Shayan2Ali Eslami3Graduated from Veterinary Parasitology, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science and Research Campus, Tehran, Iran.Department of Parasitology, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science and Research Campus, Tehran, Iran.Department of Parasitology, Faculty of Specialized Veterinary Science, Tehran Uneversity, Tehran, IranDepartment of Parasitology, Faculty of Specialized Veterinary Science, Tehran Uneversity, Tehran, Iran.In a trial, in Tabriz area, the ability of an experimental vaccine from the larval homogenate of Ascaridia galli was evaluated during the year 1384. A group of 5 white leghorn hens were immunized twice with 100 micrograms larval homogenate of Ascaridia galli diluted in 1ml PBS and emulsified in 1ml Freund’s adjuvant orally. Booster immunization was also administered orally on day 21 The other group (5 white leghorn hens) received 1 ml PBS emulsified in 1ml of the same adjuvant. On day 33 each hen was administered approximately 10,000 Ascaridia galli infective eggs orally. Blood samples were collected from the animals in a 10 day interval from the first immunization until infective challenge and weekly thereafter until the end of study. Sera were tested by ELISA and Western-blotting. Hens were necropsied 8 weeks post challenge with infective eggs for recovery of the nematodes. Vaccinated hens showed a 76 percent reduction in mean EPG, 74 percent reduction in the average number of male worms, 79 percent reduction in the average number of female worms and 77 percent reduction in the mean number of total worms. Significance in mean optical density of sera in ELISA was noticed (phttp://jvcp.iaut.ac.ir/article_518631_3a64b7d5e7ce08eb8fcac22966aeb03b.pdfAscaridia galliimmunizationwhite leghorn hen |
| spellingShingle | Yaghob Gharehdaghi Naser Hoghooghi Rad Parviz Shayan Ali Eslami Identification and determination of effective and immunogenic antigens of Ascaridia galli in poultry reared in Tabriz area Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī Ascaridia galli immunization white leghorn hen |
| title | Identification and determination of effective and immunogenic antigens of Ascaridia galli in poultry reared in Tabriz area |
| title_full | Identification and determination of effective and immunogenic antigens of Ascaridia galli in poultry reared in Tabriz area |
| title_fullStr | Identification and determination of effective and immunogenic antigens of Ascaridia galli in poultry reared in Tabriz area |
| title_full_unstemmed | Identification and determination of effective and immunogenic antigens of Ascaridia galli in poultry reared in Tabriz area |
| title_short | Identification and determination of effective and immunogenic antigens of Ascaridia galli in poultry reared in Tabriz area |
| title_sort | identification and determination of effective and immunogenic antigens of ascaridia galli in poultry reared in tabriz area |
| topic | Ascaridia galli immunization white leghorn hen |
| url | http://jvcp.iaut.ac.ir/article_518631_3a64b7d5e7ce08eb8fcac22966aeb03b.pdf |
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