Identification of the egusi seed trait locus (eg) and its suppressor gene associated with the thin seed coat trait in watermelon
Egusi watermelon has a unique egusi seed type, which could be useful for breeding both edible seeds and edible flesh in watermelon. However, the genetic basis of the unique egusi seed type is not clear. In the present study, we first reported that at least two genes with inhibitory epistasis were re...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1018975/full |
| _version_ | 1811175322449608704 |
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| author | Na Li Dan Zhou Nannan Li Shengnan Kong Jianli Shang Wanting Zhu Jiming Wang Shuangwu Ma |
| author_facet | Na Li Dan Zhou Nannan Li Shengnan Kong Jianli Shang Wanting Zhu Jiming Wang Shuangwu Ma |
| author_sort | Na Li |
| collection | DOAJ |
| description | Egusi watermelon has a unique egusi seed type, which could be useful for breeding both edible seeds and edible flesh in watermelon. However, the genetic basis of the unique egusi seed type is not clear. In the present study, we first reported that at least two genes with inhibitory epistasis were responsible for the thin seed coat (unique egusi seed type) in watermelon. Inheritance analysis of five populations, including F2, BC, and BCF2, suggested that the thin seed coat trait was controlled by a suppressor gene together with the egusi seed locus (eg) in egusi watermelon. Based on high-throughput sequencing technology, two quantitative trait loci located on chromosome 1 and chromosome 6 were identified for the thin seed coat trait in watermelon. One of the loci, the eg locus on chromosome 6, was finely mapped to a genomic region of 15.7 kb, which contained only one candidate gene. Comparative transcriptome analysis highlighted differentially expressed genes involved in cellulose and lignin synthesis between watermelon genotypes varying in the thickness of the seed coat and provided several potential candidate genes for the thin seed coat trait. Taken together, our data suggest that at least two genes are complementarily involved in the thin seed coat trait and will be useful for cloning novel genes. The results presented here provide a new reference for uncovering egusi seed genetic mechanisms and valuable information for marker-assisted selection in seed coat breeding. |
| first_indexed | 2024-04-10T19:34:08Z |
| format | Article |
| id | doaj.art-1fd53d628a5d438eb6aca13d2f9ed10b |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T19:34:08Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1fd53d628a5d438eb6aca13d2f9ed10b2023-01-30T08:19:41ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011410.3389/fpls.2023.10189751018975Identification of the egusi seed trait locus (eg) and its suppressor gene associated with the thin seed coat trait in watermelonNa LiDan ZhouNannan LiShengnan KongJianli ShangWanting ZhuJiming WangShuangwu MaEgusi watermelon has a unique egusi seed type, which could be useful for breeding both edible seeds and edible flesh in watermelon. However, the genetic basis of the unique egusi seed type is not clear. In the present study, we first reported that at least two genes with inhibitory epistasis were responsible for the thin seed coat (unique egusi seed type) in watermelon. Inheritance analysis of five populations, including F2, BC, and BCF2, suggested that the thin seed coat trait was controlled by a suppressor gene together with the egusi seed locus (eg) in egusi watermelon. Based on high-throughput sequencing technology, two quantitative trait loci located on chromosome 1 and chromosome 6 were identified for the thin seed coat trait in watermelon. One of the loci, the eg locus on chromosome 6, was finely mapped to a genomic region of 15.7 kb, which contained only one candidate gene. Comparative transcriptome analysis highlighted differentially expressed genes involved in cellulose and lignin synthesis between watermelon genotypes varying in the thickness of the seed coat and provided several potential candidate genes for the thin seed coat trait. Taken together, our data suggest that at least two genes are complementarily involved in the thin seed coat trait and will be useful for cloning novel genes. The results presented here provide a new reference for uncovering egusi seed genetic mechanisms and valuable information for marker-assisted selection in seed coat breeding.https://www.frontiersin.org/articles/10.3389/fpls.2023.1018975/fullegusi seedsuppressor genemarker-assisted selectioncandidate geneseed coatwatermelon |
| spellingShingle | Na Li Dan Zhou Nannan Li Shengnan Kong Jianli Shang Wanting Zhu Jiming Wang Shuangwu Ma Identification of the egusi seed trait locus (eg) and its suppressor gene associated with the thin seed coat trait in watermelon Frontiers in Plant Science egusi seed suppressor gene marker-assisted selection candidate gene seed coat watermelon |
| title | Identification of the egusi seed trait locus (eg) and its suppressor gene associated with the thin seed coat trait in watermelon |
| title_full | Identification of the egusi seed trait locus (eg) and its suppressor gene associated with the thin seed coat trait in watermelon |
| title_fullStr | Identification of the egusi seed trait locus (eg) and its suppressor gene associated with the thin seed coat trait in watermelon |
| title_full_unstemmed | Identification of the egusi seed trait locus (eg) and its suppressor gene associated with the thin seed coat trait in watermelon |
| title_short | Identification of the egusi seed trait locus (eg) and its suppressor gene associated with the thin seed coat trait in watermelon |
| title_sort | identification of the egusi seed trait locus eg and its suppressor gene associated with the thin seed coat trait in watermelon |
| topic | egusi seed suppressor gene marker-assisted selection candidate gene seed coat watermelon |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1018975/full |
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