Phenolic profile and biological activities of Aloe barbadensis (Miller) from western Algeria
Aloe vera is widely used in conventional medicine in Algeria to treat various diseases. This study aims to evaluate the chemical composition and biological activities of Aloe vera collected from western Algeria. Two extracts of ethanolic (EEA) and aqueous (AEA) were used to determine the total phen...
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| Format: | Article |
| Language: | English |
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TMKarpinski Publisher, Tomasz M. Karpiński
2022-11-01
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| Series: | European Journal of Biological Research |
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| Online Access: | http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/592 |
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| author | Firdaous Faiza Fedoul Boumediene Meddah Mohammed Larouci Aicha Tir Touil Yahya Merazi Yavuz Selim Cakmak |
| author_facet | Firdaous Faiza Fedoul Boumediene Meddah Mohammed Larouci Aicha Tir Touil Yahya Merazi Yavuz Selim Cakmak |
| author_sort | Firdaous Faiza Fedoul |
| collection | DOAJ |
| description |
Aloe vera is widely used in conventional medicine in Algeria to treat various diseases. This study aims to evaluate the chemical composition and biological activities of Aloe vera collected from western Algeria. Two extracts of ethanolic (EEA) and aqueous (AEA) were used to determine the total phenolic and flavonoid content. HPLC was applied to determine the amount of 15 compounds they contain, while the antioxidant activity was determined by the DPPH method. The antimicrobial activity experiment was conducted against five selected bacterial strains. Finally, an in vivo study on Swiss albino mice was conducted to discover the toxicity using Lorke’s method and anti-inflammatory activity using the Carrageenan method. The EEA extract shows the highest total phenol content of 37.00±0.37mg GAE/g and total flavonoid content of 9.14±0.19 mg CE/g. The AEA contains hydroxybenzoic and benzoic acid with other ingredients (0.84 and 0.82 mg/g, respectively). The EEA contains 0.93 mg/g of benzoic acid. Aloe vera has antioxidant activity with IC50 values equal to 0.821 mg/ml for EEA and 1.993 mg/ml for AEA. The AEA inhibits E. coli and S. aureus with a bacteriostatic effect; EEA is the best inhibitor of S. aureus and S. mutans with the bactericidal effect. Aloe vera is practically nontoxic (LD50 is 3800 mg/kg of the AEA and superior to 5000 mg/kg of EEA). The AEA gives the best inhibition of edema, 85.96% at (100 mg/kg). Aloe vera leaves are an important resource of polyphenols, which have interesting antioxidant power, and antimicrobial and anti-inflammatory activities.
DOI: http://dx.doi.org/10.5281/zenodo.7274997
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| first_indexed | 2024-04-13T17:06:48Z |
| format | Article |
| id | doaj.art-1fd997d0161d4d9c81983b06377fde29 |
| institution | Directory Open Access Journal |
| issn | 2449-8955 |
| language | English |
| last_indexed | 2024-04-13T17:06:48Z |
| publishDate | 2022-11-01 |
| publisher | TMKarpinski Publisher, Tomasz M. Karpiński |
| record_format | Article |
| series | European Journal of Biological Research |
| spelling | doaj.art-1fd997d0161d4d9c81983b06377fde292022-12-22T02:38:27ZengTMKarpinski Publisher, Tomasz M. KarpińskiEuropean Journal of Biological Research2449-89552022-11-01124Phenolic profile and biological activities of Aloe barbadensis (Miller) from western AlgeriaFirdaous Faiza Fedoul0Boumediene Meddah1Mohammed Larouci2Aicha Tir Touil3Yahya Merazi4Yavuz Selim Cakmak5Bioconversion, Microbiological, Engineering, and Health Safety Laboratory, Department of Biology, Faculty of Nature and Life Sciences, University of Mustapha Stambouli Mascara, 29000 Mascara, AlgeriaBioconversion, Microbiological, Engineering, and Health Safety Laboratory, Department of Biology, Faculty of Nature and Life Sciences, University of Mustapha Stambouli Mascara, 29000 Mascara, AlgeriaBioconversion, Microbiological, Engineering, and Health Safety Laboratory, Department of Biology, Faculty of Nature and Life Sciences, University of Mustapha Stambouli Mascara, 29000 Mascara, AlgeriaBioconversion, Microbiological, Engineering, and Health Safety Laboratory, Department of Biology, Faculty of Nature and Life Sciences, University of Mustapha Stambouli Mascara, 29000 Mascara, AlgeriaDepartment of Biology; Faculty of Nature and Life Sciences, University Abdelhamid Ibn Badis Mostaganem, AlgeriaDepartment of Biotechnology and Molecular Biology, Faculty of Science and Letters, Aksaray University, Aksaray, Turkey Aloe vera is widely used in conventional medicine in Algeria to treat various diseases. This study aims to evaluate the chemical composition and biological activities of Aloe vera collected from western Algeria. Two extracts of ethanolic (EEA) and aqueous (AEA) were used to determine the total phenolic and flavonoid content. HPLC was applied to determine the amount of 15 compounds they contain, while the antioxidant activity was determined by the DPPH method. The antimicrobial activity experiment was conducted against five selected bacterial strains. Finally, an in vivo study on Swiss albino mice was conducted to discover the toxicity using Lorke’s method and anti-inflammatory activity using the Carrageenan method. The EEA extract shows the highest total phenol content of 37.00±0.37mg GAE/g and total flavonoid content of 9.14±0.19 mg CE/g. The AEA contains hydroxybenzoic and benzoic acid with other ingredients (0.84 and 0.82 mg/g, respectively). The EEA contains 0.93 mg/g of benzoic acid. Aloe vera has antioxidant activity with IC50 values equal to 0.821 mg/ml for EEA and 1.993 mg/ml for AEA. The AEA inhibits E. coli and S. aureus with a bacteriostatic effect; EEA is the best inhibitor of S. aureus and S. mutans with the bactericidal effect. Aloe vera is practically nontoxic (LD50 is 3800 mg/kg of the AEA and superior to 5000 mg/kg of EEA). The AEA gives the best inhibition of edema, 85.96% at (100 mg/kg). Aloe vera leaves are an important resource of polyphenols, which have interesting antioxidant power, and antimicrobial and anti-inflammatory activities. DOI: http://dx.doi.org/10.5281/zenodo.7274997 http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/592Aloe veraAntimicrobialAntioxidantAnti-inflammatoryHPLC |
| spellingShingle | Firdaous Faiza Fedoul Boumediene Meddah Mohammed Larouci Aicha Tir Touil Yahya Merazi Yavuz Selim Cakmak Phenolic profile and biological activities of Aloe barbadensis (Miller) from western Algeria European Journal of Biological Research Aloe vera Antimicrobial Antioxidant Anti-inflammatory HPLC |
| title | Phenolic profile and biological activities of Aloe barbadensis (Miller) from western Algeria |
| title_full | Phenolic profile and biological activities of Aloe barbadensis (Miller) from western Algeria |
| title_fullStr | Phenolic profile and biological activities of Aloe barbadensis (Miller) from western Algeria |
| title_full_unstemmed | Phenolic profile and biological activities of Aloe barbadensis (Miller) from western Algeria |
| title_short | Phenolic profile and biological activities of Aloe barbadensis (Miller) from western Algeria |
| title_sort | phenolic profile and biological activities of aloe barbadensis miller from western algeria |
| topic | Aloe vera Antimicrobial Antioxidant Anti-inflammatory HPLC |
| url | http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/592 |
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