Identification of the hub susceptibility genes and related common transcription factors in the skeletal muscle of Type 2 Diabetes Mellitus
Abstract Background Type 2 diabetes mellitus (T2DM) and its related complications contribute to the high morbidity and mortality in worldwide. Skeletal muscle insulin resistance plays a critical role in the onset of T2DM due to the decreasing in the insulin-stimulated glucose uptake. T2DM is associa...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Endocrine Disorders |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12902-022-01195-0 |
| _version_ | 1798018441610788864 |
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| author | Jianjuan Ke Xiaohua Hu Changhua Wang Yemin Zhang |
| author_facet | Jianjuan Ke Xiaohua Hu Changhua Wang Yemin Zhang |
| author_sort | Jianjuan Ke |
| collection | DOAJ |
| description | Abstract Background Type 2 diabetes mellitus (T2DM) and its related complications contribute to the high morbidity and mortality in worldwide. Skeletal muscle insulin resistance plays a critical role in the onset of T2DM due to the decreasing in the insulin-stimulated glucose uptake. T2DM is associated not only with the inherited factors but also with the noninherited factors. However, the susceptibility genes related with the two factors and the transcription factors (TF) regulating the susceptibility genes in skeletal muscle, which aggravate the development of T2DM were still ill-defined. Methods In the present study, the expression profiles by the array of GSE25462 were retrieved from the GEO database. GEO2R was performed to validate the susceptibility differentially expressed genes (SDEG) in skeletal muscle of T2DM. Gene Ontology (GO) analysis and The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were conducted via The Database for Annotation, Visualization, and Integrated Discovery (DAVID). A Protein-Protein Interaction (PPI) network was performed with the STRING. Results With the performance of GEO2R, 229 SDEGs in skeletal muscle of T2DM were identified. The biological processes (BP) of SDEGs was enriched in the cellular response to UV-B most significantly. KEGG pathway analysis revealed that the SDEGs were most significantly enriched in glycosaminoglycan degradation. 5 hub susceptibility genes (GPR84, CALCB, GCG, PTGDR, GNG8) in the skeletal muscle of T2DM were identified. Eventually, the common transcription factors regulating the hub susceptibility genes were identified by means of the online tool PROMO. Conclusions Five hub susceptibility genes (GPR84, CALCB, GCG, PTGDR, GNG8) in the skeletal muscle of T2DM and the common transcription factors were identified. The outputs would provide new clues on the novel potential targets and the therapeutic strategies for treating T2DM and its related diseases. |
| first_indexed | 2024-04-11T16:24:11Z |
| format | Article |
| id | doaj.art-1fdbbc5d33d34f7faab13bab0d7d390c |
| institution | Directory Open Access Journal |
| issn | 1472-6823 |
| language | English |
| last_indexed | 2024-04-11T16:24:11Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Endocrine Disorders |
| spelling | doaj.art-1fdbbc5d33d34f7faab13bab0d7d390c2022-12-22T04:14:14ZengBMCBMC Endocrine Disorders1472-68232022-11-0122111410.1186/s12902-022-01195-0Identification of the hub susceptibility genes and related common transcription factors in the skeletal muscle of Type 2 Diabetes MellitusJianjuan Ke0Xiaohua Hu1Changhua Wang2Yemin Zhang3Department of Anesthesiology, Zhongnan Hospital of Wuhan UniversityDepartment of Respiratory Medicine, Renmin Hospital of LichuanDepartment of Pathology & Pathophysiology, Wuhan University Taikang Medical School (School of Basic Medical Sciences)Department of Pathology & Pathophysiology, Wuhan University Taikang Medical School (School of Basic Medical Sciences)Abstract Background Type 2 diabetes mellitus (T2DM) and its related complications contribute to the high morbidity and mortality in worldwide. Skeletal muscle insulin resistance plays a critical role in the onset of T2DM due to the decreasing in the insulin-stimulated glucose uptake. T2DM is associated not only with the inherited factors but also with the noninherited factors. However, the susceptibility genes related with the two factors and the transcription factors (TF) regulating the susceptibility genes in skeletal muscle, which aggravate the development of T2DM were still ill-defined. Methods In the present study, the expression profiles by the array of GSE25462 were retrieved from the GEO database. GEO2R was performed to validate the susceptibility differentially expressed genes (SDEG) in skeletal muscle of T2DM. Gene Ontology (GO) analysis and The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were conducted via The Database for Annotation, Visualization, and Integrated Discovery (DAVID). A Protein-Protein Interaction (PPI) network was performed with the STRING. Results With the performance of GEO2R, 229 SDEGs in skeletal muscle of T2DM were identified. The biological processes (BP) of SDEGs was enriched in the cellular response to UV-B most significantly. KEGG pathway analysis revealed that the SDEGs were most significantly enriched in glycosaminoglycan degradation. 5 hub susceptibility genes (GPR84, CALCB, GCG, PTGDR, GNG8) in the skeletal muscle of T2DM were identified. Eventually, the common transcription factors regulating the hub susceptibility genes were identified by means of the online tool PROMO. Conclusions Five hub susceptibility genes (GPR84, CALCB, GCG, PTGDR, GNG8) in the skeletal muscle of T2DM and the common transcription factors were identified. The outputs would provide new clues on the novel potential targets and the therapeutic strategies for treating T2DM and its related diseases.https://doi.org/10.1186/s12902-022-01195-0T2DMSusceptibility differentially expressed genes (SDEG)Transcription factorsBioinformaticsInherited factorsNoninherited factors |
| spellingShingle | Jianjuan Ke Xiaohua Hu Changhua Wang Yemin Zhang Identification of the hub susceptibility genes and related common transcription factors in the skeletal muscle of Type 2 Diabetes Mellitus BMC Endocrine Disorders T2DM Susceptibility differentially expressed genes (SDEG) Transcription factors Bioinformatics Inherited factors Noninherited factors |
| title | Identification of the hub susceptibility genes and related common transcription factors in the skeletal muscle of Type 2 Diabetes Mellitus |
| title_full | Identification of the hub susceptibility genes and related common transcription factors in the skeletal muscle of Type 2 Diabetes Mellitus |
| title_fullStr | Identification of the hub susceptibility genes and related common transcription factors in the skeletal muscle of Type 2 Diabetes Mellitus |
| title_full_unstemmed | Identification of the hub susceptibility genes and related common transcription factors in the skeletal muscle of Type 2 Diabetes Mellitus |
| title_short | Identification of the hub susceptibility genes and related common transcription factors in the skeletal muscle of Type 2 Diabetes Mellitus |
| title_sort | identification of the hub susceptibility genes and related common transcription factors in the skeletal muscle of type 2 diabetes mellitus |
| topic | T2DM Susceptibility differentially expressed genes (SDEG) Transcription factors Bioinformatics Inherited factors Noninherited factors |
| url | https://doi.org/10.1186/s12902-022-01195-0 |
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