A Bacteriocin with Broad Antimicrobial Activity Produced by Newly Isolated Nitrogen-Fixing Bacillus Strains
Background: Recently re-emerging infections occur due to antibiotic-resistant bacteria. The use of bacteriocins as a substitute for currently used antibiotics is promising. The isolated nitrogen-fixing Bacillus bacteria might be a potential candidate strain as an alternative source of peptide antibi...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2013-02-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/2029 |
| _version_ | 1797722319046574080 |
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| author | Saeideh Zaghian Giti Emtiazi Dariush Shokri |
| author_facet | Saeideh Zaghian Giti Emtiazi Dariush Shokri |
| author_sort | Saeideh Zaghian |
| collection | DOAJ |
| description | Background: Recently re-emerging infections occur due to antibiotic-resistant bacteria. The use of bacteriocins as a substitute for currently used antibiotics is promising. The isolated nitrogen-fixing Bacillus bacteria might be a potential candidate strain as an alternative source of peptide antibiotics in the future.
Methods: A total of 15 nitrogen-fixing Bacillus strains with antibacterial activity were isolated from soil samples. Bacteriocin-producing strains against the indicator strains were co-cultured and isolated. The antibacterial activity of bacteriocin was detected by spot-on-the lawn method with different bacteria. Effects of proteolytic enzymes, pH, and heat treatment on bacteriocin activity were also determined.
Findings: All isolated spore forming Bacillus strains had an inhibitory effect on Listeria monocytogenes and Staphylococcus aureus. Some of them had an inhibitory effect on methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE). None of them had an inhibitory effect on Gram-negative bacteria. The antimicrobial compounds produced by these novel strains were inactivated by the proteolytic enzymes which demonstrated their proteinaceous nature. These bacteriocins were also active in a wide range of pH and temperature.
Conclusion: In the present study, new bacteriocin-producing nitrogen-fixing Bacillus strains with anti-MRSA and anti-VRE activity were isolated from the environment and their biochemical properties were determined.
Keywords: Nitrogen-fixing Bacillus strains, Bacteriocin, Methicillin-resistant Staphylococcus aureus, Vancomycin-resistant Enterococcus faecalis |
| first_indexed | 2024-03-12T09:46:50Z |
| format | Article |
| id | doaj.art-1fdc3efdf3b14f42a1b53ff320a85531 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T09:46:50Z |
| publishDate | 2013-02-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-1fdc3efdf3b14f42a1b53ff320a855312023-09-02T12:50:32ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2013-02-0130218226022691236A Bacteriocin with Broad Antimicrobial Activity Produced by Newly Isolated Nitrogen-Fixing Bacillus StrainsSaeideh Zaghian0Giti Emtiazi1Dariush Shokri2Department of Biology, School of Sciences, University of Isfahan, Isfahan, IranProfessor, Department of Biology, School of Sciences, University of Isfahan, Isfahan, IranPhD Candidate, Department of Biology, School of Sciences, University of Isfahan, Isfahan, IranBackground: Recently re-emerging infections occur due to antibiotic-resistant bacteria. The use of bacteriocins as a substitute for currently used antibiotics is promising. The isolated nitrogen-fixing Bacillus bacteria might be a potential candidate strain as an alternative source of peptide antibiotics in the future. Methods: A total of 15 nitrogen-fixing Bacillus strains with antibacterial activity were isolated from soil samples. Bacteriocin-producing strains against the indicator strains were co-cultured and isolated. The antibacterial activity of bacteriocin was detected by spot-on-the lawn method with different bacteria. Effects of proteolytic enzymes, pH, and heat treatment on bacteriocin activity were also determined. Findings: All isolated spore forming Bacillus strains had an inhibitory effect on Listeria monocytogenes and Staphylococcus aureus. Some of them had an inhibitory effect on methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE). None of them had an inhibitory effect on Gram-negative bacteria. The antimicrobial compounds produced by these novel strains were inactivated by the proteolytic enzymes which demonstrated their proteinaceous nature. These bacteriocins were also active in a wide range of pH and temperature. Conclusion: In the present study, new bacteriocin-producing nitrogen-fixing Bacillus strains with anti-MRSA and anti-VRE activity were isolated from the environment and their biochemical properties were determined. Keywords: Nitrogen-fixing Bacillus strains, Bacteriocin, Methicillin-resistant Staphylococcus aureus, Vancomycin-resistant Enterococcus faecalishttp://jims.mui.ac.ir/index.php/jims/article/view/2029 |
| spellingShingle | Saeideh Zaghian Giti Emtiazi Dariush Shokri A Bacteriocin with Broad Antimicrobial Activity Produced by Newly Isolated Nitrogen-Fixing Bacillus Strains مجله دانشکده پزشکی اصفهان |
| title | A Bacteriocin with Broad Antimicrobial Activity Produced by Newly Isolated Nitrogen-Fixing Bacillus Strains |
| title_full | A Bacteriocin with Broad Antimicrobial Activity Produced by Newly Isolated Nitrogen-Fixing Bacillus Strains |
| title_fullStr | A Bacteriocin with Broad Antimicrobial Activity Produced by Newly Isolated Nitrogen-Fixing Bacillus Strains |
| title_full_unstemmed | A Bacteriocin with Broad Antimicrobial Activity Produced by Newly Isolated Nitrogen-Fixing Bacillus Strains |
| title_short | A Bacteriocin with Broad Antimicrobial Activity Produced by Newly Isolated Nitrogen-Fixing Bacillus Strains |
| title_sort | bacteriocin with broad antimicrobial activity produced by newly isolated nitrogen fixing bacillus strains |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/2029 |
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