Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat?
We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2019-10-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35230 |
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| author | Jeferson Fereira da Fonseca Ribrio Ivan Tavares Pereira Batista Juliane Teramachi Trevizan Joanna Maria Gonçalves Souza-Fabjan Felipe Zandonadi Brandão Luiz Sérgio Almeida Camargo |
| author_facet | Jeferson Fereira da Fonseca Ribrio Ivan Tavares Pereira Batista Juliane Teramachi Trevizan Joanna Maria Gonçalves Souza-Fabjan Felipe Zandonadi Brandão Luiz Sérgio Almeida Camargo |
| author_sort | Jeferson Fereira da Fonseca |
| collection | DOAJ |
| description | We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student’s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat’s uterus could present an alternative to nuclear maturation. |
| first_indexed | 2024-04-10T20:41:13Z |
| format | Article |
| id | doaj.art-1fde400a86a4429d9c13e4a5fd0927de |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-04-10T20:41:13Z |
| publishDate | 2019-10-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-1fde400a86a4429d9c13e4a5fd0927de2023-01-24T19:41:28ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592019-10-01406Supl310.5433/1679-0359.2019v40n6Supl3p3789Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat?Jeferson Fereira da Fonseca0Ribrio Ivan Tavares Pereira Batista1Juliane Teramachi Trevizan2Joanna Maria Gonçalves Souza-Fabjan3Felipe Zandonadi Brandão4Luiz Sérgio Almeida Camargo5Empresa Brasileira de Pesquisa AgropecuáriaUniversidade Federal FluminenseEscola de Agricultura e Ciências VeterináriasUniversidade Federal FluminenseUniversidade Federal FluminenseEmpresa Brasileira de Pesquisa AgropecuáriaWe used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student’s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat’s uterus could present an alternative to nuclear maturation.https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35230COCsBovine oocytesIn vitro maturationEx-situ maturationNonsurgical embryo transfer techniqueGoat. |
| spellingShingle | Jeferson Fereira da Fonseca Ribrio Ivan Tavares Pereira Batista Juliane Teramachi Trevizan Joanna Maria Gonçalves Souza-Fabjan Felipe Zandonadi Brandão Luiz Sérgio Almeida Camargo Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat? Semina: Ciências Agrárias COCs Bovine oocytes In vitro maturation Ex-situ maturation Nonsurgical embryo transfer technique Goat. |
| title | Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat? |
| title_full | Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat? |
| title_fullStr | Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat? |
| title_full_unstemmed | Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat? |
| title_short | Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat? |
| title_sort | goat incubator can bovine oocytes be matured in the uterine horn of a goat |
| topic | COCs Bovine oocytes In vitro maturation Ex-situ maturation Nonsurgical embryo transfer technique Goat. |
| url | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35230 |
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