Effect of Heat Stress on Egg Production, Steroid Hormone Synthesis, and Related Gene Expression in Chicken Preovulatory Follicular Granulosa Cells
This study was conducted to elucidate the molecular mechanisms underlying heat stress (HS)-induced abnormal egg-laying in laying hens. Hy-Line brown laying hens were exposed to HS at 32 °C or maintained at 22 °C (control) for 14 days. In addition, granulosa cells (GCs) from preovulatory follicles we...
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MDPI AG
2022-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/11/1467 |
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| author | Leyan Yan Mengdie Hu Lihong Gu Mingming Lei Zhe Chen Huanxi Zhu Rong Chen |
| author_facet | Leyan Yan Mengdie Hu Lihong Gu Mingming Lei Zhe Chen Huanxi Zhu Rong Chen |
| author_sort | Leyan Yan |
| collection | DOAJ |
| description | This study was conducted to elucidate the molecular mechanisms underlying heat stress (HS)-induced abnormal egg-laying in laying hens. Hy-Line brown laying hens were exposed to HS at 32 °C or maintained at 22 °C (control) for 14 days. In addition, granulosa cells (GCs) from preovulatory follicles were subjected to normal (37 °C) or high (41 °C or 43 °C) temperatures in vitro. Proliferation, apoptosis, and steroidogenesis were investigated, and the expression of estrogen and progesterone synthesis-related genes was detected. The results confirmed that laying hens reared under HS had impaired laying performance. HS inhibited proliferation, increased apoptosis, and altered the GC ultrastructure. HS also elevated progesterone secretion by increasing the expression of steroidogenic acute regulatory protein (StAR), cytochrome P450 family 11 subfamily A member 1 (CYP11A1), and 3b-hydroxysteroid dehydrogenase (3β-HSD). In addition, HS inhibited estrogen synthesis in GCs by decreasing the expression of the follicle-stimulating hormone receptor (FSHR) and cytochrome P450 family 19 subfamily A member 1 (CYP19A1). The upregulation of heat shock 70 kDa protein (HSP70) under HS was also observed. Collectively, laying hens exposed to high temperatures experienced damage to follicular GCs and steroidogenesis dysfunction, which reduced their laying performance. This study provides a molecular mechanism for the abnormal laying performance of hens subjected to HS. |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T01:32:17Z |
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| spelling | doaj.art-1fdec21999a643fab31c5e424377ce0f2023-11-23T13:39:01ZengMDPI AGAnimals2076-26152022-06-011211146710.3390/ani12111467Effect of Heat Stress on Egg Production, Steroid Hormone Synthesis, and Related Gene Expression in Chicken Preovulatory Follicular Granulosa CellsLeyan Yan0Mengdie Hu1Lihong Gu2Mingming Lei3Zhe Chen4Huanxi Zhu5Rong Chen6Key Laboratory for Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Animal Husbandry Institute, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaKey Laboratory for Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Animal Husbandry Institute, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou 571100, ChinaKey Laboratory for Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Animal Husbandry Institute, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaKey Laboratory for Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Animal Husbandry Institute, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaKey Laboratory for Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Animal Husbandry Institute, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaKey Laboratory for Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Animal Husbandry Institute, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaThis study was conducted to elucidate the molecular mechanisms underlying heat stress (HS)-induced abnormal egg-laying in laying hens. Hy-Line brown laying hens were exposed to HS at 32 °C or maintained at 22 °C (control) for 14 days. In addition, granulosa cells (GCs) from preovulatory follicles were subjected to normal (37 °C) or high (41 °C or 43 °C) temperatures in vitro. Proliferation, apoptosis, and steroidogenesis were investigated, and the expression of estrogen and progesterone synthesis-related genes was detected. The results confirmed that laying hens reared under HS had impaired laying performance. HS inhibited proliferation, increased apoptosis, and altered the GC ultrastructure. HS also elevated progesterone secretion by increasing the expression of steroidogenic acute regulatory protein (StAR), cytochrome P450 family 11 subfamily A member 1 (CYP11A1), and 3b-hydroxysteroid dehydrogenase (3β-HSD). In addition, HS inhibited estrogen synthesis in GCs by decreasing the expression of the follicle-stimulating hormone receptor (FSHR) and cytochrome P450 family 19 subfamily A member 1 (CYP19A1). The upregulation of heat shock 70 kDa protein (HSP70) under HS was also observed. Collectively, laying hens exposed to high temperatures experienced damage to follicular GCs and steroidogenesis dysfunction, which reduced their laying performance. This study provides a molecular mechanism for the abnormal laying performance of hens subjected to HS.https://www.mdpi.com/2076-2615/12/11/1467heat stressgranulosa cellsprogesteroneestradiolStARlaying hens |
| spellingShingle | Leyan Yan Mengdie Hu Lihong Gu Mingming Lei Zhe Chen Huanxi Zhu Rong Chen Effect of Heat Stress on Egg Production, Steroid Hormone Synthesis, and Related Gene Expression in Chicken Preovulatory Follicular Granulosa Cells Animals heat stress granulosa cells progesterone estradiol StAR laying hens |
| title | Effect of Heat Stress on Egg Production, Steroid Hormone Synthesis, and Related Gene Expression in Chicken Preovulatory Follicular Granulosa Cells |
| title_full | Effect of Heat Stress on Egg Production, Steroid Hormone Synthesis, and Related Gene Expression in Chicken Preovulatory Follicular Granulosa Cells |
| title_fullStr | Effect of Heat Stress on Egg Production, Steroid Hormone Synthesis, and Related Gene Expression in Chicken Preovulatory Follicular Granulosa Cells |
| title_full_unstemmed | Effect of Heat Stress on Egg Production, Steroid Hormone Synthesis, and Related Gene Expression in Chicken Preovulatory Follicular Granulosa Cells |
| title_short | Effect of Heat Stress on Egg Production, Steroid Hormone Synthesis, and Related Gene Expression in Chicken Preovulatory Follicular Granulosa Cells |
| title_sort | effect of heat stress on egg production steroid hormone synthesis and related gene expression in chicken preovulatory follicular granulosa cells |
| topic | heat stress granulosa cells progesterone estradiol StAR laying hens |
| url | https://www.mdpi.com/2076-2615/12/11/1467 |
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