κ-Carrageenan Hydrogel as a Matrix for Therapeutic Enzyme Immobilization
During the last few decades, polysaccharide hydrogels attract more and more attention as therapeutic protein delivery systems due to their biocompatibility and the simplicity of the biodegradation of natural polymers. The protein retention by and release from the polysaccharide gel network is regula...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/19/4071 |
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| author | Olga N. Makshakova Liliya R. Bogdanova Anastasiya O. Makarova Aleksandra M. Kusova Elena A. Ermakova Mariia A. Kazantseva Yuriy F. Zuev |
| author_facet | Olga N. Makshakova Liliya R. Bogdanova Anastasiya O. Makarova Aleksandra M. Kusova Elena A. Ermakova Mariia A. Kazantseva Yuriy F. Zuev |
| author_sort | Olga N. Makshakova |
| collection | DOAJ |
| description | During the last few decades, polysaccharide hydrogels attract more and more attention as therapeutic protein delivery systems due to their biocompatibility and the simplicity of the biodegradation of natural polymers. The protein retention by and release from the polysaccharide gel network is regulated by geometry and physical interactions of protein with the matrix. In the present work, we studied the molecular details of interactions between κ-carrageenan and three lipases, namely the lipases from <i>Candida rugosa</i>, <i>Mucor javanicus</i>, and <i>Rhizomucor miehei</i>—which differ in their size and net charge—upon protein immobilization in microparticles of polysaccharide gel. The kinetics of protein release revealed the different capability of κ-carrageenan to retain lipases, which are generally negatively charged; that was shown to be in line with the energy of interactions between polysaccharides and positively charged epitopes on the protein surface. These data create a platform for the novel design of nanocarriers for biomedical probes of enzymatic origin. |
| first_indexed | 2024-03-09T21:15:39Z |
| format | Article |
| id | doaj.art-1fe055ae58a64518a412b7885bf157a9 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T21:15:39Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-1fe055ae58a64518a412b7885bf157a92023-11-23T21:33:45ZengMDPI AGPolymers2073-43602022-09-011419407110.3390/polym14194071κ-Carrageenan Hydrogel as a Matrix for Therapeutic Enzyme ImmobilizationOlga N. Makshakova0Liliya R. Bogdanova1Anastasiya O. Makarova2Aleksandra M. Kusova3Elena A. Ermakova4Mariia A. Kazantseva5Yuriy F. Zuev6Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, Lobachevsky St., 2/31, 420111 Kazan, RussiaKazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, Lobachevsky St., 2/31, 420111 Kazan, RussiaKazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, Lobachevsky St., 2/31, 420111 Kazan, RussiaKazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, Lobachevsky St., 2/31, 420111 Kazan, RussiaKazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, Lobachevsky St., 2/31, 420111 Kazan, RussiaKazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, Lobachevsky St., 2/31, 420111 Kazan, RussiaKazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, Lobachevsky St., 2/31, 420111 Kazan, RussiaDuring the last few decades, polysaccharide hydrogels attract more and more attention as therapeutic protein delivery systems due to their biocompatibility and the simplicity of the biodegradation of natural polymers. The protein retention by and release from the polysaccharide gel network is regulated by geometry and physical interactions of protein with the matrix. In the present work, we studied the molecular details of interactions between κ-carrageenan and three lipases, namely the lipases from <i>Candida rugosa</i>, <i>Mucor javanicus</i>, and <i>Rhizomucor miehei</i>—which differ in their size and net charge—upon protein immobilization in microparticles of polysaccharide gel. The kinetics of protein release revealed the different capability of κ-carrageenan to retain lipases, which are generally negatively charged; that was shown to be in line with the energy of interactions between polysaccharides and positively charged epitopes on the protein surface. These data create a platform for the novel design of nanocarriers for biomedical probes of enzymatic origin.https://www.mdpi.com/2073-4360/14/19/4071polysaccharidesnanogelsenzymesimmobilizationdelivery systems |
| spellingShingle | Olga N. Makshakova Liliya R. Bogdanova Anastasiya O. Makarova Aleksandra M. Kusova Elena A. Ermakova Mariia A. Kazantseva Yuriy F. Zuev κ-Carrageenan Hydrogel as a Matrix for Therapeutic Enzyme Immobilization Polymers polysaccharides nanogels enzymes immobilization delivery systems |
| title | κ-Carrageenan Hydrogel as a Matrix for Therapeutic Enzyme Immobilization |
| title_full | κ-Carrageenan Hydrogel as a Matrix for Therapeutic Enzyme Immobilization |
| title_fullStr | κ-Carrageenan Hydrogel as a Matrix for Therapeutic Enzyme Immobilization |
| title_full_unstemmed | κ-Carrageenan Hydrogel as a Matrix for Therapeutic Enzyme Immobilization |
| title_short | κ-Carrageenan Hydrogel as a Matrix for Therapeutic Enzyme Immobilization |
| title_sort | κ carrageenan hydrogel as a matrix for therapeutic enzyme immobilization |
| topic | polysaccharides nanogels enzymes immobilization delivery systems |
| url | https://www.mdpi.com/2073-4360/14/19/4071 |
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