Proteomic Analysis of Dendritic Filopodia-Rich Fraction Isolated by Telencephalin and Vitronectin Interaction
Dendritic filopodia are thin, long, and highly mobile protrusions functioning as spine precursors. By contrast with a wealth of knowledge on molecular profiles in spines, little is known about structural and functional proteins present in dendritic filopodia. To reveal the molecular constituents of...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-08-01
|
| Series: | Frontiers in Synaptic Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fnsyn.2018.00027/full |
| _version_ | 1819205848342200320 |
|---|---|
| author | Yutaka Furutani Yoshihiro Yoshihara Yoshihiro Yoshihara |
| author_facet | Yutaka Furutani Yoshihiro Yoshihara Yoshihiro Yoshihara |
| author_sort | Yutaka Furutani |
| collection | DOAJ |
| description | Dendritic filopodia are thin, long, and highly mobile protrusions functioning as spine precursors. By contrast with a wealth of knowledge on molecular profiles in spines, little is known about structural and functional proteins present in dendritic filopodia. To reveal the molecular constituents of dendritic filopodia, we developed a new method for biochemical preparation of proteins enriched in dendritic filopodia, by taking advantage of specific and strong binding between a dendritic filopodial membrane protein, telencephalin, and its extracellular matrix ligand, vitronectin. When vitronectin-coated magnetic microbeads were added onto cultured hippocampal neurons, phagocytic cup-like membrane protrusions were formed on dendrites through the binding to telencephalin. Magnetically purified membrane protrusion fraction was subjected to comprehensive mass spectrometric analysis and 319 proteins were identified, many of which were confirmed to be localized to dendritic filopodia. Thus, this study provides a useful resource for studying molecular mechanisms underlying dendritic development, synapse formation, and plasticity. |
| first_indexed | 2024-12-23T04:58:13Z |
| format | Article |
| id | doaj.art-1fe55584166f4e26a93a989aee72bf96 |
| institution | Directory Open Access Journal |
| issn | 1663-3563 |
| language | English |
| last_indexed | 2024-12-23T04:58:13Z |
| publishDate | 2018-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Synaptic Neuroscience |
| spelling | doaj.art-1fe55584166f4e26a93a989aee72bf962022-12-21T17:59:17ZengFrontiers Media S.A.Frontiers in Synaptic Neuroscience1663-35632018-08-011010.3389/fnsyn.2018.00027344046Proteomic Analysis of Dendritic Filopodia-Rich Fraction Isolated by Telencephalin and Vitronectin InteractionYutaka Furutani0Yoshihiro Yoshihara1Yoshihiro Yoshihara2Laboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, Saitama, JapanLaboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, Saitama, JapanLaboratory for Systems Molecular Ethology, RIKEN Center for Brain Science, Saitama, JapanDendritic filopodia are thin, long, and highly mobile protrusions functioning as spine precursors. By contrast with a wealth of knowledge on molecular profiles in spines, little is known about structural and functional proteins present in dendritic filopodia. To reveal the molecular constituents of dendritic filopodia, we developed a new method for biochemical preparation of proteins enriched in dendritic filopodia, by taking advantage of specific and strong binding between a dendritic filopodial membrane protein, telencephalin, and its extracellular matrix ligand, vitronectin. When vitronectin-coated magnetic microbeads were added onto cultured hippocampal neurons, phagocytic cup-like membrane protrusions were formed on dendrites through the binding to telencephalin. Magnetically purified membrane protrusion fraction was subjected to comprehensive mass spectrometric analysis and 319 proteins were identified, many of which were confirmed to be localized to dendritic filopodia. Thus, this study provides a useful resource for studying molecular mechanisms underlying dendritic development, synapse formation, and plasticity.https://www.frontiersin.org/article/10.3389/fnsyn.2018.00027/fulltelencephalinICAM-5vitronectinproteomicsdendritic filopodia |
| spellingShingle | Yutaka Furutani Yoshihiro Yoshihara Yoshihiro Yoshihara Proteomic Analysis of Dendritic Filopodia-Rich Fraction Isolated by Telencephalin and Vitronectin Interaction Frontiers in Synaptic Neuroscience telencephalin ICAM-5 vitronectin proteomics dendritic filopodia |
| title | Proteomic Analysis of Dendritic Filopodia-Rich Fraction Isolated by Telencephalin and Vitronectin Interaction |
| title_full | Proteomic Analysis of Dendritic Filopodia-Rich Fraction Isolated by Telencephalin and Vitronectin Interaction |
| title_fullStr | Proteomic Analysis of Dendritic Filopodia-Rich Fraction Isolated by Telencephalin and Vitronectin Interaction |
| title_full_unstemmed | Proteomic Analysis of Dendritic Filopodia-Rich Fraction Isolated by Telencephalin and Vitronectin Interaction |
| title_short | Proteomic Analysis of Dendritic Filopodia-Rich Fraction Isolated by Telencephalin and Vitronectin Interaction |
| title_sort | proteomic analysis of dendritic filopodia rich fraction isolated by telencephalin and vitronectin interaction |
| topic | telencephalin ICAM-5 vitronectin proteomics dendritic filopodia |
| url | https://www.frontiersin.org/article/10.3389/fnsyn.2018.00027/full |
| work_keys_str_mv | AT yutakafurutani proteomicanalysisofdendriticfilopodiarichfractionisolatedbytelencephalinandvitronectininteraction AT yoshihiroyoshihara proteomicanalysisofdendriticfilopodiarichfractionisolatedbytelencephalinandvitronectininteraction AT yoshihiroyoshihara proteomicanalysisofdendriticfilopodiarichfractionisolatedbytelencephalinandvitronectininteraction |