Essential procedures of single-cell RNA sequencing in multiple myeloma and its translational value
Multiple myeloma (MM) is a malignant neoplasm characterized by clonal proliferation of abnormal plasma cells. In many countries, it ranks as the second most prevalent malignant neoplasm of the hematopoietic system. Although treatment methods for MM have been continuously improved and the survival of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Health
2023-10-01
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| Series: | Blood Science |
| Online Access: | http://journals.lww.com/10.1097/BS9.0000000000000172 |
| _version_ | 1797449543733739520 |
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| author | Jun Du Xiao-Ran Gu Xiao-Xiao Yu Yang-Jia Cao Jian Hou |
| author_facet | Jun Du Xiao-Ran Gu Xiao-Xiao Yu Yang-Jia Cao Jian Hou |
| author_sort | Jun Du |
| collection | DOAJ |
| description | Multiple myeloma (MM) is a malignant neoplasm characterized by clonal proliferation of abnormal plasma cells. In many countries, it ranks as the second most prevalent malignant neoplasm of the hematopoietic system. Although treatment methods for MM have been continuously improved and the survival of patients has been dramatically prolonged, MM remains an incurable disease with a high probability of recurrence. As such, there are still many challenges to be addressed. One promising approach is single-cell RNA sequencing (scRNA-seq), which can elucidate the transcriptome heterogeneity of individual cells and reveal previously unknown cell types or states in complex tissues. In this review, we outlined the experimental workflow of scRNA-seq in MM, listed some commonly used scRNA-seq platforms and analytical tools. In addition, with the advent of scRNA-seq, many studies have made new progress in the key molecular mechanisms during MM clonal evolution, cell interactions and molecular regulation in the microenvironment, and drug resistance mechanisms in target therapy. We summarized the main findings and sequencing platforms for applying scRNA-seq to MM research and proposed broad directions for targeted therapies based on these findings. |
| first_indexed | 2024-03-09T14:26:33Z |
| format | Article |
| id | doaj.art-1fe7405db3f04f6b96dd612b49048aa5 |
| institution | Directory Open Access Journal |
| issn | 2543-6368 |
| language | English |
| last_indexed | 2024-03-09T14:26:33Z |
| publishDate | 2023-10-01 |
| publisher | Wolters Kluwer Health |
| record_format | Article |
| series | Blood Science |
| spelling | doaj.art-1fe7405db3f04f6b96dd612b49048aa52023-11-28T07:05:42ZengWolters Kluwer HealthBlood Science2543-63682023-10-015422123610.1097/BS9.0000000000000172202312000-00001Essential procedures of single-cell RNA sequencing in multiple myeloma and its translational valueJun Du0Xiao-Ran Gu1Xiao-Xiao Yu2Yang-Jia Cao3Jian Hou4a Department of Hematology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, Chinab School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, Chinab School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, Chinac Department of Hematology, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shanxi 710000, Chinaa Department of Hematology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, ChinaMultiple myeloma (MM) is a malignant neoplasm characterized by clonal proliferation of abnormal plasma cells. In many countries, it ranks as the second most prevalent malignant neoplasm of the hematopoietic system. Although treatment methods for MM have been continuously improved and the survival of patients has been dramatically prolonged, MM remains an incurable disease with a high probability of recurrence. As such, there are still many challenges to be addressed. One promising approach is single-cell RNA sequencing (scRNA-seq), which can elucidate the transcriptome heterogeneity of individual cells and reveal previously unknown cell types or states in complex tissues. In this review, we outlined the experimental workflow of scRNA-seq in MM, listed some commonly used scRNA-seq platforms and analytical tools. In addition, with the advent of scRNA-seq, many studies have made new progress in the key molecular mechanisms during MM clonal evolution, cell interactions and molecular regulation in the microenvironment, and drug resistance mechanisms in target therapy. We summarized the main findings and sequencing platforms for applying scRNA-seq to MM research and proposed broad directions for targeted therapies based on these findings.http://journals.lww.com/10.1097/BS9.0000000000000172 |
| spellingShingle | Jun Du Xiao-Ran Gu Xiao-Xiao Yu Yang-Jia Cao Jian Hou Essential procedures of single-cell RNA sequencing in multiple myeloma and its translational value Blood Science |
| title | Essential procedures of single-cell RNA sequencing in multiple myeloma and its translational value |
| title_full | Essential procedures of single-cell RNA sequencing in multiple myeloma and its translational value |
| title_fullStr | Essential procedures of single-cell RNA sequencing in multiple myeloma and its translational value |
| title_full_unstemmed | Essential procedures of single-cell RNA sequencing in multiple myeloma and its translational value |
| title_short | Essential procedures of single-cell RNA sequencing in multiple myeloma and its translational value |
| title_sort | essential procedures of single cell rna sequencing in multiple myeloma and its translational value |
| url | http://journals.lww.com/10.1097/BS9.0000000000000172 |
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