Expression of the Bovine NK-Lysin Gene Family and Activity against Respiratory Pathogens.
Unlike the genomes of many mammals that have a single NK-lysin gene, the cattle genome contains a family of four genes, one of which is expressed preferentially in the lung. In this study, we compared the expression of the four bovine NK-lysin genes in healthy animals to animals challenged with path...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4943647?pdf=render |
| _version_ | 1818855003659436032 |
|---|---|
| author | Junfeng Chen Chingyuan Yang Polyana C Tizioto Huan Huang Mi O K Lee Harold R Payne Sara D Lawhon Friedhelm Schroeder Jeremy F Taylor James E Womack |
| author_facet | Junfeng Chen Chingyuan Yang Polyana C Tizioto Huan Huang Mi O K Lee Harold R Payne Sara D Lawhon Friedhelm Schroeder Jeremy F Taylor James E Womack |
| author_sort | Junfeng Chen |
| collection | DOAJ |
| description | Unlike the genomes of many mammals that have a single NK-lysin gene, the cattle genome contains a family of four genes, one of which is expressed preferentially in the lung. In this study, we compared the expression of the four bovine NK-lysin genes in healthy animals to animals challenged with pathogens known to be associated with bovine respiratory disease (BRD) using transcriptome sequencing (RNA-seq). The expression of several NK-lysins, especially NK2C, was elevated in challenged relative to control animals. The effects of synthetic peptides corresponding to functional region helices 2 and 3 of each gene product were tested on both model membranes and bio-membranes. Circular dichroism spectroscopy indicated that these peptides adopted a more helical secondary structure upon binding to an anionic model membrane and liposome leakage assays suggested that these peptides disrupt membranes. Bacterial killing assays further confirmed the antimicrobial effects of these peptides on BRD-associated bacteria, including both Pasteurella multocida and Mannhemia haemolytica and an ultrastructural examination of NK-lysin-treated P. multocida cells by transmission electron microscopy revealed the lysis of target membranes. These studies demonstrate that the expanded bovine NK-lysin gene family is potentially important in host defense against pathogens involved in bovine respiratory disease. |
| first_indexed | 2024-12-19T08:01:42Z |
| format | Article |
| id | doaj.art-1fe7e7dbfb1149a5853e22b0056c566d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T08:01:42Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1fe7e7dbfb1149a5853e22b0056c566d2022-12-21T20:29:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01117e015888210.1371/journal.pone.0158882Expression of the Bovine NK-Lysin Gene Family and Activity against Respiratory Pathogens.Junfeng ChenChingyuan YangPolyana C TiziotoHuan HuangMi O K LeeHarold R PayneSara D LawhonFriedhelm SchroederJeremy F TaylorJames E WomackUnlike the genomes of many mammals that have a single NK-lysin gene, the cattle genome contains a family of four genes, one of which is expressed preferentially in the lung. In this study, we compared the expression of the four bovine NK-lysin genes in healthy animals to animals challenged with pathogens known to be associated with bovine respiratory disease (BRD) using transcriptome sequencing (RNA-seq). The expression of several NK-lysins, especially NK2C, was elevated in challenged relative to control animals. The effects of synthetic peptides corresponding to functional region helices 2 and 3 of each gene product were tested on both model membranes and bio-membranes. Circular dichroism spectroscopy indicated that these peptides adopted a more helical secondary structure upon binding to an anionic model membrane and liposome leakage assays suggested that these peptides disrupt membranes. Bacterial killing assays further confirmed the antimicrobial effects of these peptides on BRD-associated bacteria, including both Pasteurella multocida and Mannhemia haemolytica and an ultrastructural examination of NK-lysin-treated P. multocida cells by transmission electron microscopy revealed the lysis of target membranes. These studies demonstrate that the expanded bovine NK-lysin gene family is potentially important in host defense against pathogens involved in bovine respiratory disease.http://europepmc.org/articles/PMC4943647?pdf=render |
| spellingShingle | Junfeng Chen Chingyuan Yang Polyana C Tizioto Huan Huang Mi O K Lee Harold R Payne Sara D Lawhon Friedhelm Schroeder Jeremy F Taylor James E Womack Expression of the Bovine NK-Lysin Gene Family and Activity against Respiratory Pathogens. PLoS ONE |
| title | Expression of the Bovine NK-Lysin Gene Family and Activity against Respiratory Pathogens. |
| title_full | Expression of the Bovine NK-Lysin Gene Family and Activity against Respiratory Pathogens. |
| title_fullStr | Expression of the Bovine NK-Lysin Gene Family and Activity against Respiratory Pathogens. |
| title_full_unstemmed | Expression of the Bovine NK-Lysin Gene Family and Activity against Respiratory Pathogens. |
| title_short | Expression of the Bovine NK-Lysin Gene Family and Activity against Respiratory Pathogens. |
| title_sort | expression of the bovine nk lysin gene family and activity against respiratory pathogens |
| url | http://europepmc.org/articles/PMC4943647?pdf=render |
| work_keys_str_mv | AT junfengchen expressionofthebovinenklysingenefamilyandactivityagainstrespiratorypathogens AT chingyuanyang expressionofthebovinenklysingenefamilyandactivityagainstrespiratorypathogens AT polyanactizioto expressionofthebovinenklysingenefamilyandactivityagainstrespiratorypathogens AT huanhuang expressionofthebovinenklysingenefamilyandactivityagainstrespiratorypathogens AT mioklee expressionofthebovinenklysingenefamilyandactivityagainstrespiratorypathogens AT haroldrpayne expressionofthebovinenklysingenefamilyandactivityagainstrespiratorypathogens AT saradlawhon expressionofthebovinenklysingenefamilyandactivityagainstrespiratorypathogens AT friedhelmschroeder expressionofthebovinenklysingenefamilyandactivityagainstrespiratorypathogens AT jeremyftaylor expressionofthebovinenklysingenefamilyandactivityagainstrespiratorypathogens AT jamesewomack expressionofthebovinenklysingenefamilyandactivityagainstrespiratorypathogens |